Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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LP9M80-H Isolated from Liriope platyphylla Could Help Alleviate Diabetic Symptoms via the Regulation of Glucose and Lipid Concentration

OLETF 당뇨모델동물을 이용한 맥문동 추출물(LP9M80-H)의 당뇨질환에 대한 효능

  • Kim, Ji-Eun (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Hwang, In-Sik (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Goo, Jun-Seo (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Nam, So-Hee (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Choi, Sun-Il (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Hae-Ryun (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Young-Ju (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University) ;
  • Kim, Yoon-Han (Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University) ;
  • Park, Se-Jin (Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University) ;
  • Kim, Nahm-Su (Technology Cooperation Bureau, Rural Development Administration) ;
  • Choi, Young-Hwan (Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University) ;
  • Hwang, Dae-Youn (Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University)
  • 김지은 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 황인식 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 구준서 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 남소희 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 최선일 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 이혜련 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 이영주 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 김윤한 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 박세진 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 김남수 (농촌진흥청) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 황대연 (부산대학교 생명자원과학대학 바이오소재과학과)
  • Received : 2012.02.28
  • Accepted : 2012.03.27
  • Published : 2012.05.30
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Abstract

It was reported that the novel compounds (LP9M80-H) of $Liriope$ $platyphylla$ regulate glucose transporter (Glut) biosynthesis by activating the insulin-signaling pathway in the liver and brain of ICR mice. To investigate the therapeutic effects of LP9M80-H on the pathology of diabetes and obesity, alterations of key factors related to symptoms were analyzed in the Otsuka Long Evans Tokushima Fatty (OLETF) rats treated with LP9M80-H for 2 weeks. The abdominal fat masses in the LP9M80-H-treated group were lower than the vehicle-treated group, although there was no difference in body weight between the two groups. Additionally, when compared to the vehicle-treated group, LP9M80-H treatment induced a significant decrease in glucose levels and an increase in the insulin concentration in the blood of OLETF rats. A high level of insulin protein was also detected in pancreatic ${\beta}$ cells of LP9M80-H-treated OLETF rats. A significant reduction in the concentration of lipids and adiponectin was detected only in LP9M80-H-treated OLETF rats. Furthermore, the expression of insulin receptor ${\beta}$ and the insulin receptor substrate (IRS) was dramatically decreased in LP9M80-H-treated OLETF rats compared to the vehicle-treated group. Of the glucose transporters located downstream of the insulin-signaling pathway, glucose transporters (Glut) -2 and -3 were significantly decreased in LP9M80-H-treated OLETF rats, while the level of Glut-4 was maintained under all conditions. Therefore, these results suggest that LP9M80-H may contribute to relieving symptoms of diabetes and obesity through glucose homeostasis and regulation of lipid concentration.

LP9M80-H는 맥문동($Liriope$ $platyphylla$)으로부터 메탄올과 헥산을 이용하여 추출한 새로운 추출물로서 ICR 마우스에서 인슐린분비를 촉진하며, 간과 뇌 조직에서 인슐린 신호경로를 활성화시키는 것으로 알려져 있다. 본 연구에서는 LP9M80-H가 당뇨와 비만의 치료에 미치는 효과를 조사하기 위하여, OLETF 모델동물에 LP9M80-H를 2주간 투여한 후 당뇨와 비만과 관련된 주요인자의 변화를 관찰하였다. 비록 체중은 두 집단간에 차이가 없었으나 복부 지방량은 vehicle 투여군보다 LP9M80-H 투여군에서 적었다. 또한, 혈중 포도당농도는 LP9M80-H를 투여한 OLETF 랫드가 대조군에 비하여 약간 낮았으나 인슐린의 농도는 유의적으로 크게 증가하였다. 혈청 내 3가지 주요 지질의 농도는 LP9M80-H를 투여한 OLETF 랫드에서 유의적으로 감소하였고, 지방의 산화를 촉진하는 아디포넥틴의 농도도 LP9M80-H를 투여한 OLETF 랫드에서 감소하였다. 더불어, 체내에 분비된 인슐린이 표적장기에 미치는 영향을 관찰하기 위하여 간조직에서 인슐린 수용체와 인슐린 수용체기질(iRS)의 발현을 관찰하였으며, 이러한 2가지 단백질은 LP9M80-H를 투여한 OLETF 랫드에서 vehicle 투여군에 비해 유의미하게 감소하였다. 또한, 인슐린 신호 경로의 다운스트림에 위치하는 포도당 수송체 중에서 Glut-2와 Glut-3 발현은 LP9M80-H를 투여한 OLETF 랫드에서 유의미하게 감소하는 반면에, Glut-4 발현은 일정하게 유지되었다. 따라서 이러한 결과는 LP9M80-H는 포도당 항상성과 지질농도의 조절을 통하여 당뇨와 비만의 증상을 완화시키는데 기여할 것으로 사료된다.

Keywords

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