Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Repeated-Dose Oral Toxicity Study of Lactobacillus Plantarum AF1 Isolated from Kimchi in Rats

김치에서 분리한 Lactobacillus plantarum AF1의 흰쥐에 대한 반복투여독성

  • Received : 2012.03.02
  • Accepted : 2012.03.30
  • Published : 2012.05.31
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Abstract

This study was performed to investigate the 4-week repeated-dose toxicity of $Lactobacillus$ $plantarum$ AF1 ($Lb.$ $plantarum$ AF1), a lactic acid bacteria isolated from kimchi, in male and female rats. Sprague-Dawley male and female rats were divided into four groups, with 10 animals in each group. The test article was administered once daily by gavage to rats at dosage levels of 0, 0.5, 1.0, and 2.0 g/kg/day for 4-weeks. There were no test articlerelated deaths or abnormal clinical signs in either gender of rat during the observation period. Furthermore, no differences were found between the control and treatment groups in terms of body weight changes, food intake, and water consumptions. Hematological parameters, serum biochemical analysis, and any other findings also showed no significant or dose-dependent alterations. There were no alterations in organ weights upon administration of $Lb.$ $plantarum$ AF1. These results suggest that there were no adverse effects of oral application of $Lb.$ $plantarum$ AF1 up to a dosage level of 2.0 g/kg in both male and female rats.

본 연구는 항진균 및 항세균 활성이 있는 김치 유래 유산균인 $Lb.$ $plantarum$ AF1을 가지고 천연항균제로서 사용 적합성 및 안정성을 평가하고자 $Lb.$ $plantarum$ AF1을 SD 계통의 흰쥐에게 4주간 반복 경구투여를 통하여 장기투여에 의한 안전성을 확인하고자 식품의약품안전청의 의약품 등의 독성시험기준에 따라 실시하였다. SD 계열의 암수 흰쥐에 시험물질을 0, 500, 1,000 및 2,000 mg/kg/day의 용량으로 4주간 반복 경구투여한 후 사망률, 일반증상, 체중변화, 사료 섭취량, 수분섭취량, 혈액학적 및 혈액생화학적 변화, 부검 소견 및 장기무게의 변화를 관찰하였다. 모든 시험군에서 시험기간 시험물질로 인한 일반증상 및 사망동뮬이 관찰되지 않았다. 전 시험기간 동안 $Lb.$ $plantarum$ AF1의 4주간의 경구투여 결과 체중이 지속적으로 증가되었지만 대조군과 유의성 있는 변화가 관찰되지 않았다. 또한 혈액학적, 혈액생화학적 검사, 장기의 육안적 관찰, 장기 중량변화 및 조직 병리학적 소견에서도 모든 시험물질 투여군이 대조군과 유의성이 있는 차이를 보이지 않았으며, 모두 정상 범위를 벗어나지 않아 시험물질에 기인하는 이상 소견을 발견할 수 없었다. 이상의 결과로 보아 4주 반복투여 독성시험 결과 항진균 및 항세균 활성을 지닌 유산균인 $Lb.$ $plantarum$ AF1은 저독성 물질로 판정되었으며, 안전성 확인을 통하여 천연항균제로의 개발이 가능할 것으로 사료된다.

Keywords

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