Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지
DOI QR코드

DOI QR Code

IN VITRO EVALUATION OF EXPERIMENTAL FLUORIDE TAPE IN INHIBITION OF ENAMEL DEMINERALIZATION

불소 테이프의 법랑질 탈회 억제 효과에 관한 실험적 평가

  • Kim, Min-Jung (Department of Pediatric Dentistry, Graduate School of Dentistry, Chosun University) ;
  • Lee, Sang-Ho (Department of Pediatric Dentistry, Graduate School of Dentistry, Chosun University) ;
  • Lee, Nan-Young (Department of Pediatric Dentistry, Graduate School of Dentistry, Chosun University) ;
  • Park, Seung-Hyo (Department of Pediatric Dentistry, Graduate School of Dentistry, Chosun University)
  • 김민정 (조선대학교 치의학전문대학원 소아치과학교실) ;
  • 이상호 (조선대학교 치의학전문대학원 소아치과학교실) ;
  • 이난영 (조선대학교 치의학전문대학원 소아치과학교실) ;
  • 박승효 (조선대학교 치의학전문대학원 소아치과학교실)
  • Received : 2012.02.06
  • Accepted : 2012.05.10
  • Published : 2012.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this in vitro study was to evaluate the effectiveness of experimental 2.26% fluoride-polyvinyl alcohol (F-PVA) tape in inhibition of enamel demineralization using enamel surface microhardness (SMH) analysis and scanning electron microscopy (SEM) examination. Enamel specimens (n=60) randomly assigned to four groups: control group, F-PVA tape group, fluoride varinish (F-varnish) group, casein phosphopeptide-amorphous calcium phosphate (CPP-ACFP) group. After topical application, pH-cycling was processed. Then, SMH was measured and the percentage loss of surface microhardness (%SML) was calculated. For the SEM examination, five sample specimens in each group were treated and the morphologic character was evaluated. After pH-cycling, the SMH values of the enamel specimens of F-PVA tape and F-varnish group were significantly higher than that of CPP-ACFP group, there was no significant difference between F-PVA tape and Fvarnish group. With SEM examination, enamel surfaces in the F-PVA tape group and F-varnish group showed numerous spherical and ovoid crystals formed on the enamel surface were also observed. The density of crystals was higher than that of both control group and CPP-ACFP group. F-PVA tape is effective in inhibition of enamel demineralization. Also, F-PVA tape's inhibition of enamel demineralization is comparable to that of F-vanish and greater than that of CPP-ACFP.

본 연구의 목적은 법랑질 표면 미세경도의 측정과 주사전자현미경 관찰을 통해 본 교실에서 개발한 2.26%의 불소를 함유한 폴리비닐알코올 테잎 (F-PVA tape)의 법랑질 탈회 억제 효과를 평가하기 위한 것이다. 60개의 법랑질 표본을 각 군당 15개 씩, 임의로 대조군, F-PVA tape 군, 불소바니쉬 군, CPP-ACFP 군으로 나누고, 각각의 재광화 제재를 도포한 후 pH-cycling 과정 거쳤다. 표본의 표면 미세경도를 측정하고 표면 미세경도 소실량을 계산하였다. 주사전자현미경 관찰을 위해, 각 군에서 5개의 표본을 추출하고 형태학적 특징을 분석하였고 다음과 같은 결론을 얻었다. 1. pH-cycling 후, F-PVA tape 군과 불소바니쉬 군의 법랑질 표면 미세경도 값은 CPP-ACFP 군과 비교 시 유의하게 높았으며, F-PVA tape 군과 불소바니쉬 군 사이에는 유의한 차이가 없었다. 2. 주사전자현미경 관찰 시, F-PVA tape 군과 불소바니쉬 군에서는 법랑질 표면에 형성된 수많은 구형 및 난원형의 결정 입자들이 관찰되었으며, 결정 입자의 밀도는 대조군 및 CPP-ACFP 군에서 보다 높았다. F-PVA tape은 법랑질의 탈회를 억제하는데 효과적이며, 그 효과는 불소바니쉬와 견줄만하고, CPP-ACFP 보다 우수하다.

Keywords

References

  1. Zhang Q, Zou J, Yang R, et al. : Remineralization effects of casein phosphopeptide-amorphous calcium phosphate cre｀me on artificial early enamel lesions of primary teeth. Int J Paediatr Dent, 21:374-381, 2011. https://doi.org/10.1111/j.1365-263X.2011.01135.x
  2. Cochrane NJ, Cai F, Huq NL, et al. : New approaches to enhanced remineralization of tooth enamel. J Dent Res, 89:1187-1197, 2010. https://doi.org/10.1177/0022034510376046
  3. Borges BC, de Souza Borges J, de Araujo LS, et al. : Update on nonsurgical, ultraconservative approaches to treat effectively non-cavitated caries lesions in permanent teeth. Eur J Dent, 5:229-236, 2011.
  4. Brunelle JA, Carlos JP : Recent trends in dental caries in U.S. children and the effect of water fluoridation. J Dent Res, 69:723-727, 1990.
  5. Lang WP, Weintraub JA, Choi C, et al. : Fissure sealant knowledge and characteristics of parents as a function of their child's sealant status. J Public Health Dent, 48:133-137, 1988. https://doi.org/10.1111/j.1752-7325.1988.tb03182.x
  6. Tinanoff N : Dental caries risk assessment and prevention. Dent Clin North Am, 39:709-719, 1995.
  7. Salar DV, Garcia-Godoy F, Flaitz CM, et al. : Potential inhibition of demineralization in vitro by fluoride-releasing sealants. J Am Dent Assoc, 138:502-506, 2007. https://doi.org/10.14219/jada.archive.2007.0203
  8. 권호근, 김백일, 이영희 등 : 불소치약 사용후 시간 변화에 따른 구강내 타액의 불소농도변화에 관한 연구. 대한구강보건학회지, 20:555-567, 1996.
  9. Kawai F, Hu X : Biochemistry of microbial polyvinyl alcohol degradation. Appl Microbiol Biotechnol, 84:227-237, 2009. https://doi.org/10.1007/s00253-009-2113-6
  10. Jiang S, Liu S, Feng W : PVA hydrogel properties for biomedical application. J Mech Behav Biomed Mater, 4:1228-1233, 2011. https://doi.org/10.1016/j.jmbbm.2011.04.005
  11. 최준호 : 폴리비닐알콜 및 셀룰로오스계 혼합 고분자를 이용한 항균 및 분해도 조절 연구. 광주, 조선대 대학원, 2, 2011.
  12. 임성옥, 이상호, 이난영, 박승효 : 불소를 함유한 PVA 고분자 접착 테잎의 불소 유리 효과. 대한소아치과학회지, 38:327-336, 2011.
  13. Lata S, Varghese NO, Varughese JM : Remineralization potential of fluoride and amorphous calcium phosphate-casein phospho peptide on enamel lesions: An in vitro comparative evaluation. J Conserv Dent, 13:42-46, 2010. https://doi.org/10.4103/0972-0707.62634
  14. Cheng L, ten Cate JM : Effect of Galla chinensis on the in vitro remineralization of advanced enamel lesions. Int J Oral Sci, 2:15-20, 2010. https://doi.org/10.4248/IJOS10019
  15. Magalhaes AC, Stancari FH, Rios D, et al. : Effect of an experimental 4% titanium tetrafluoride varnish on dental erosion by a soft drink. J Dent, 35:858-861, 2007. https://doi.org/10.1016/j.jdent.2007.08.005
  16. Murakami C, Bonecker M, Correa MS, et al. : Effect of fluoride varnish and gel on dental erosion in primary and permanent teeth. Arch Oral Biol, 54:997-1001, 2009. https://doi.org/10.1016/j.archoralbio.2009.08.003
  17. Delbem AC, Bergamaschi M, Sassaki KT, et al. : Effect of fluoridated varnish and silver diamine fluoride solution on enamel demineralization: pH-cycling study. J Appl Oral Sci, 14:88-92, 2006.
  18. Featherstone JD, ten Cate JM, Shariati M, et al. : Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res, 17:385-391, 1983. https://doi.org/10.1159/000260692
  19. Meredith N, Sherriff M, Setchell DJ, et al. : Measurement of the microhardness and Young's modulus of human enamel and dentine using an indentation technique. Arch Oral Biol, 41:539-545, 1996. https://doi.org/10.1016/0003-9969(96)00020-9
  20. ten Cate JM, Duijsters PP : Alternating demineralization and remineralization of artificial enamel lesions. Caries Res, 16:201-210, 1982. https://doi.org/10.1159/000260599
  21. Zero DT : Dentifrices, mouthwashes, and remineralization/caries arrestment strategies. BMC Oral Health, 6:S9, 2006. https://doi.org/10.1186/1472-6831-6-S1-S9
  22. Gibson G, Jurasic MM, Wehler CJ, et al. : Supplemental fluoride use for moderate and high caries risk adults: a systematic review. J Public Health Dent, 71:171-184, 2011.
  23. Quock RL, Warren-Morris DP : Fluoride varnish: the top choice for professionally applied fluoride. J Mich Dent Assoc, 93:42-48, 2011.
  24. Petersson LG, Arthursson L, Ostberg C : Cariesinhibiting effects of different modes of Duraphat varnish reapplication: a 3-year radiographic study. Caries Res, 25:70-73, 1991. https://doi.org/10.1159/000261345
  25. Reynolds EC : Casein phosphopeptide-amorphous calcium phosphate: the scientific evidence. Adv Dent Res, 21:25-29, 2009. https://doi.org/10.1177/0895937409335619
  26. 박승효, 이상호, 이난영 : 불소 테잎 적용 후 시간변화에 따른 타액 내 불소농도. 대한소아치과학회지, 36:377-386,2009.
  27. Srinivasan N, Kavitha M, Loganathan SC : Comparison of the remineralization potential of CPP-ACP and CPP-ACP with 900 ppm fluoride on eroded human enamel: An in situ study. Arch Oral Biol, 55:541-544, 2010. https://doi.org/10.1016/j.archoralbio.2010.05.002
  28. Jayarajan J, Janardhanam P, Jayakumar P : Efficacy of CPP-ACP and CPP-ACPF on enamel remineralization - an in vitro study using scanning electron microscope and DIAGNOdent. Indian J Dent Res, 22:77-82, 2011. https://doi.org/10.4103/0970-9290.80001
  29. Castillo JL, Milgrom P, Kharasch E, et al. : Evaluation of fluoride release from commercially available fluoride varnishes. J Am Dent Assoc, 132:1389-1392, 2001. https://doi.org/10.14219/jada.archive.2001.0053