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Surface roughness and $Candida$ $albicans$ adhesion to flexible denture base according to various polishing methods

연마방법에 따른 탄성의치의 표면거칠기와 $Candida$ $albicans$의 부착율 변화

  • Oh, Ju-Won (Department of Prosthodontics and Institute of Oral Bio-Science, Dental School, Chonbuk National University) ;
  • Seo, Jae-Min (Department of Prosthodontics and Institute of Oral Bio-Science, Dental School, Chonbuk National University) ;
  • Ahn, Seung-Keun (Department of Prosthodontics and Institute of Oral Bio-Science, Dental School, Chonbuk National University) ;
  • Park, Ju-Mi (Department of Prosthodontics and Institute of Oral Bio-Science, Dental School, Chonbuk National University) ;
  • Kang, Cheol-Kyun (Department of Prosthodontics and Institute of Oral Bio-Science, Dental School, Chonbuk National University) ;
  • Song, Kwang-Yeob (Department of Prosthodontics and Institute of Oral Bio-Science, Dental School, Chonbuk National University)
  • 오주원 (전북대학교 치의학전문대학원 치과보철학교실 및 구강생체과학연구소) ;
  • 서재민 (전북대학교 치의학전문대학원 치과보철학교실 및 구강생체과학연구소) ;
  • 안승근 (전북대학교 치의학전문대학원 치과보철학교실 및 구강생체과학연구소) ;
  • 박주미 (전북대학교 치의학전문대학원 치과보철학교실 및 구강생체과학연구소) ;
  • 강철균 (전북대학교 치의학전문대학원 치과보철학교실 및 구강생체과학연구소) ;
  • 송광엽 (전북대학교 치의학전문대학원 치과보철학교실 및 구강생체과학연구소)
  • Received : 2012.03.14
  • Accepted : 2012.04.09
  • Published : 2012.04.30

Abstract

Purpose: The purpose of this study was to compare the effect of 3 chairside polishing methods and laboratory polishing methods on surface roughness and $C.$ $albicans$ adhesion of polyamide denture base. Materials and methods: Using contact profilometer, the surface of polyamide specimens ($25{\times}15{\times}2mm$) was studied after conventional polishing without finishing and after chiarside polishing with 2 chiarside polishing kits and chairside-pumice polishing following finishing with tungsten carbide bur. To evaluate the adhesion of $C.$ $albicans$, $C.$ $albicans$ suspension was overlayed on the test specimen. And the specimens were incubated for 2 hours. Imprint culture method was achieved and counted the colony on the agar plate. Polished polyamide were evaluated using a scanning electron microscope. The statistics were conducted using one-way ANOVA and in case of difference, Scheffe test and Tamhane's T2 test were used. Results: Surface roughness (Ra) of surfaces polished with 2 chairside polishing kits had higher than conventional polishing and pumice polishing. The highest roughness value was $0.32{\pm}0.10{\mu}m$, and the lowest was $0.02{\pm}0.00{\mu}m$. The adhesion of $C.$ $albicans$ on the specimens polished with chairside polishing group and pumice polishing group were increased than conventional polishing group ($P$<.01). Conclusion: Conventional laboratory polishing was found to produce the smoothest surface and the lowest adhesion of $C.$ $albicans$. Two groups polished with Chairside polishing kits were similar with respect to surface roughness. Surface of the specimen polished with pumice is significantly smoother than 2 chairside polishing groups, but the result of $C.$ $albicans$ adhesion is that group polished with pumice was similar with 2 chairside polishing groups ($P$>.01).

연구 목적: 본 연구는 탄성의치 제작 시 주로 사용되는 polyamide를 수종의 연마방법을 사용하여 처리 후 $Candida$ $albicans$의 부착정도와 표면거칠기를 비교하고자 하였다. 연구 재료 및 방법: $25{\times}15{\times}2mm$ 크기의 polyamide 시편을 4군으로 나누어 연마재를 사용하여 기공용 lathe 연마하는 방법(기공실내 연마방법)과 각기 다른 2종의 실리콘 포인트로 진료실내 연마하는 방법, 그리고 실리콘포인트 연마 후 pumice로 연마하는 방법으로 처리하였다. $C.$ $albicans$의 부착성을 평가하기 위해 $5{\times}10^6CFU/ml$$C.$ $albicans$ 현탁액에 시편을 2시간 동안 침적하였고 5회 수세처리 후 한천배지에서 배양하였다. 그리고 주사전자 현미경(JSM-5900, JEOL LTd., Tokyo, Japan) 촬영을 시행하였다. Profilometer (Surf-pak; Kawasaki, Japan)를 이용하여 표면거칠기를 측정하였고 통계처리를 위하여 SPSS 18.0 프로그램을 사용하였다. 일원변량분석으로 비교 분석하였고 사후 검증은 $C.$ $albicans$의 부착성 검증을 위해 Scheffe test를 시행하였으며 표면조도검증을 위해 Tamhane's T2 test를 시행하였다(${\alpha}$=.01). 결과: 최대 거칠기 값을 보인 군은 2단계의 연마용 버를 사용한 것으로 $0.32{\mu}m{\pm}0.10$ 값을 나타냈으며, 가장 낮은 거칠기 값을 보인 것은 tungsten carbide를 사용하지 않고 기공용 lathe로만 연마를 한 군으로 $0.02{\mu}m{\pm}0.00$의 거칠기 값을 나타냈다. $C.$ $albicans$ 부착 실험에서는 기공용 lathe만을 이용한 연마방법이 가장 적은 부착수를 보였으며 다른 세 군과 유의한 차이가 발견되었다($P$<.01). 결론: 표면거칠기 및 미생물 부착능 실험 결과 기공실 연마만을 시행한 경우 유의하게 낮은 거칠기 값과 부착율을 보였다. Pumice로 추가 연마한 군은 진료실연마를 시행한 군에 비해 낮은 거칠기 값을 보였으나 $C.$ $albicans$ 부착에 있어서는 유의한 차이를 보이지 않았다($P$>.01).

Keywords

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