The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Chrysanthemum (Chrysanthemum morifolium) Crown Rot Caused by Fusarium solani in Korea

  • Mun, Hye-Yeon (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Jeong, Je-Yong (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kim, Chang-Jeon (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Lee, Hyang-Burm (Division of Applied Bioscience and Biotechnology, Chonnam National University)
  • Received : 2011.10.29
  • Accepted : 2011.11.27
  • Published : 2012.03.01
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Abstract

In August 2010, a severe crown rot was observed on chrysanthemum ($Chrysanthemum$ $morifolium$ Ramat., variety Sinro) in several greenhouses located at Damyang and Muan, Jeonnam province, Korea. Three isolates (EML-CHS1, -CHS2, and -CHS3) of $Fusarium$ were isolated from the affected plants and identified based on morphological characteristics and rDNA internal transcribed spacer (ITS) sequence analysis. Sequence analysis by BLAST indicated that EMLCHS1, -CHS2 and CHS3 were closest to a $Fusarium$ species, $F.$ $solani$ with > 99% sequence similarity. Pathogenicity tests were performed on chrysanthemum with spore suspensions containing $3.4{\times}10^6$ spores/ml using the dipping method. Ten days after inoculation, similar symptoms to those observed in the greenhouses were seen on the inoculated plants. The causal fungus was reisolated from the artificially inoculated basal stems, fulfilling Koch's postulates. To our knowledge, this is the first report of crown rot by $Fusarium$ $solani$ on chrysanthemum ($Chrysanthemum$ $morifolium$) in Korea.

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References

  1. Agriculture & Landscape Program. 2011. Diseases of Chrysanthemum (Chrysanthemum, Dendranthema, Leucanthemum). University of Massachusetts Amherst, Center for Agriculture, UMass Extension, USDA, USA.
  2. Booth, C. 1971. The Genus Fusarium. pp. 46-53. CMI, Kew, Surrey, UK.
  3. Collins, R. A., Ng, T. B., Fong, W. P., Wan, C. C. and Yeung, H. W. 1997. A comparison of human immunodeficiency virus type 1 inhibition by partially purified aqueous extracts of Chinese medicinal herbs. Life Sci. 60:345-351.
  4. Farr, D. F. and Rossman, A. Y. 2011. Fungal Databases, Systematic Mycology& Microbiology Laboratory, ARS, USDA, USA. (http://nt.ars-grin.gov/fungaldatabases/).
  5. Fisher, N. L. 1983. Symptom response and colonization as measures of resistance in chrysanthemum cultivars inoculated with Fusarium oxysporum f. sp. chrysanthemi. Plant Dis. 67:376-378. https://doi.org/10.1094/PD-67-376
  6. Hu, C. Q., Chen, K., Shi, Q., Kilkuskie, R. E., Cheng, Y. C. and Lee, K. H. 1994. Anti-AIDS agents, 10. Acacetin-7-O-beta-D-galactopyranoside, an anti-HIV principle from Chrysanthemum morifolium and a structure-activity correlation with some related flavonoids. J. Nat. Prod. 57:42-51. https://doi.org/10.1021/np50103a006
  7. Lee, H. B., Park, J. Y., Jung, H. S. and Summerbell, R. C. 2006. Phaeomoniella zymoides and Phaeomoniella pinifoliorum spp. nov., new acid-tolerant epiphytic fungi isolated from pine needles in Korea. Mycologia 98:598-611. https://doi.org/10.3852/mycologia.98.4.598
  8. Marongiu, B., Piras, A., Porcedda, S., Porcedda, S., Tuveri, E., Laconi, S., Deidda, D. and Maxia, A. 2009. Chemical and biological comparisons on supercritical extracts of Tanacetum cinerariifolium (Trevir) Sch. Bip. with three related species of chrysanthemums of Sardinia (Italy). Nat. Prod. Res. 23:190-199. https://doi.org/10.1080/14786410801946221
  9. Nelson, P. E., Toussoun, T. A. and Marasas, W. F. O. 1983. Fusarium species. pp. 146-150. The Pennsylvania State University Press, USA.
  10. Ren, Y.-Z., Tan, H., Li, Z.-J., Du, J. and Li, H. 2008. First report of lavender wilt caused by Fusarium solani in China. Plant Pathol. 57:377.
  11. Sassi, A. B., Harzallah-Skhiri, F., Bourgougnon, N. and Aouni, M. 2008. Antimicrobial activities of four Tunisian Chrysanthemum species. Indian J. Med. Res. 127:183-192.
  12. Strider, D. L. 1985a. Fusarium wilt of chrysanthemum: cultivar susceptibility and chemical control. Plant Dis. 69:564-568. https://doi.org/10.1094/PD-69-564
  13. Strider, D. L. 1985b. Fusarium wilt of chrysanthemum: pathogenfree rooted cuttings and susceptibility of new cultivars. Plant Dis. 69:836-838. https://doi.org/10.1094/PD-69-836
  14. Summerbell, R. C and Schroers, H. J. 2002. Analysis of phylogenetic relationship of Cylindrocarpon lichenicola and Acremonium falciforme to the Fusarium solani species complex and a review of similarities in the spectrum of opportunistic infections caused by these fungi. J. Clin. Microbiol. 40:2866-2875. https://doi.org/10.1128/JCM.40.8.2866-2875.2002
  15. Summerell, B. A., Salleh, B. and Leslie, J. F. 2003. A utilitarian approach to Fusarium identification. Plant Dis. 87:117-128. https://doi.org/10.1094/PDIS.2003.87.2.117
  16. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599. https://doi.org/10.1093/molbev/msm092
  17. The Korean Society of Plant Pathology KSPP. List of plant diseases in Korea. 5th Eds., p. 348-351.
  18. Thompson, J. D., Gigson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876

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