The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Distribution and Antifungal Activity of Endophytic Fungi in Different Growth Stages of Chili Pepper (Capsicum annuum L.) in Korea

  • Paul, Narayan Chandra (Department of Agricultural Biology, Chungnam National University) ;
  • Deng, Jian Xin (Department of Agricultural Biology, Chungnam National University) ;
  • Sang, Hyun-Kyu (Department of Agricultural Biology, Chungnam National University) ;
  • Choi, Young-Phil (Department of Agricultural Biology, Chungnam National University) ;
  • Yu, Seung-Hun (Department of Agricultural Biology, Chungnam National University)
  • 투고 : 2011.07.05
  • 심사 : 2011.12.12
  • 발행 : 2012.03.01
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초록

This study investigated the distribution of endophytic fungi obtained from the surface sterilized tissues of leaves, stems and roots of chili pepper ($Capsicum$ $annuum$ L.) plants in seedling, flowering and fruiting stages in Korea and their antifungal activity. A total of 481 isolates were recovered and were identified using molecular techniques. Based on rDNA ITS gene sequence and phylogenetic analysis, 21 fungal genera were characterized, belonging to 16 Ascomycota and 5 Basidiomycota. $Penicillium$ in seedling stage, $Fusarium$ in flowering stage, $Colletotrichum$ followed by $Fusarium$, $Alternaria$ and $Xylaria$ in fruiting stage was predominant and $Alternaria$, $Cladosporium$ and $Fusarium$ were common in all growth stages. Among 481 endophytes 90 phenotypes were evaluated for the antimicrobial activity against three major pathogens ($Phytophthora$ $capsici$, $Colletotrichum$ $acutatum$ and $Fusarium$ $oxysporum$) of chili pepper. Among them 16 isolates inhibited the growth of at least one test microorganisms. Three strains showed a broad spectrum antifungal activity and displayed strong inhibition against chili pepper pathogenic fungi.

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참고문헌

  1. Bacon, C. W. 1990. Isolation, culture and maintenance of endophytic fungi of grasses In: Isolation of biotechnological organisms from nature, ed. by D. P. Labeda, McGraw-Hill, New York.
  2. Baltruschat, H., Fodor, J., Harrach, B. D., Niemczyk, E., Barna, B., Gullner, G., Janeczko, A., Kogel, K. H., Schafer, P., Schwarczinger, I., Zuccaro, A. and Skoczowski, A. 2008. Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants. New Phytol. 180:500-510.
  3. Benhamou, N., Garand, C. and Goulet, A. 2002. Ability of nonpathogenic Fusarium oxysporum strain Fo47 to induce resistance against Pythium ultimum infection in cucumber. Appl. Environ. Microbiol. 68:4045-4060.
  4. Benerjee, D. 2011. Endophytic fungal diversity in tropical and subtropical plants. Res. J. Microbiol. 6:54-62. https://doi.org/10.3923/jm.2011.54.62
  5. Bussaban, B., Lumyong, S., McKenzie, E. H. C. and Hyde, K. D. 2001. Endophytic fungi from Ammonium siamense. Can. J. Microbiol. 47:943-948. https://doi.org/10.1139/w01-098
  6. Camargo, J. A. 1992. Can dominance influence stability in competitive interactions?. Oikos. 64:605-609. https://doi.org/10.2307/3545183
  7. Carroll, G. C. 1988. Fungal endophytes in stems and leaves: from latent pathogens to mutualistic symbiont. Ecology 69:2-9. https://doi.org/10.2307/1943154
  8. Chen, X. M., Dong, H. L., Hu, K. X., Sun, Z. R., Chen, J. and Guo, S. X. 2010a. Diversity and antimicrobial and plant-growth-promoting activities of endophytic fungi in Dendrobium loddigesii Rolfe. J. Plant Growth Regul. 29:328-337. https://doi.org/10.1007/s00344-010-9139-y
  9. Chen, J., Hu, K. X. and Hou, X. O. 2011a. Endophytic fungi assemblages from 10 Dendrobium medicinal plants (Orchidaceae). World J. Microbiol. Biotechnol. 27:1009-1016. https://doi.org/10.1007/s11274-010-0544-y
  10. Chen, X. Y., Qi, Y. D., Wei, J. H., Zhang, Z., Wang, D. L., Feng, J. D. and Gan, B. C. 2011b. Molecular identification of endophytic fungi from medicinal plant Huperizia serrata based on rDNA ITS analysis. World J. Microbiol. Biotechnol. 27:495-503. https://doi.org/10.1007/s11274-010-0480-x
  11. Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph.D. thesis, University of Newcastle, New castle Upon Tyne, UK.
  12. Clay, K. 1989. Clavicipitaceous endophytes of grasses: their potential as biocontrol agents. Mycol. Res. 92:1-12. https://doi.org/10.1016/S0953-7562(89)80088-7
  13. Dorworth, C. E. and Callan, B. E. 1996. Manipulation of endophytic fungi to promote their utility as vegetation biocontrol agents. In: Endophytic fungi in grasses and woody plants, ed. by S. C. Reddin and L. M. Carris. APS Press, St. Paul. USA.
  14. Felsenstein, J. 1985. Confidence limits of phylogenies: An approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.2307/2408678
  15. Fisher, P. J. and Petrini, O. 1987. Location of fungal endophytes in tissues of Suaeda fruiticosa: apreliminary study. Trans. Br. Mycol. Soc. 89:246-249. https://doi.org/10.1016/S0007-1536(87)80161-4
  16. Fisher, P. J., Andoson, A. E. and Petrini, O. 1986. Fungal endophytes in Ulex europaeus and Ulex gallii. Trans. Br. Mycol. Soc. 86:153-156. https://doi.org/10.1016/S0007-1536(86)80128-0
  17. Fisher, P. J., Petrini, O. and Lappin, S. H. M. 1992. The distribution of some fungal and bacterial endophytes in maize (Zea mays L.). New Phytol. 122:299-305. https://doi.org/10.1111/j.1469-8137.1992.tb04234.x
  18. Flor, N. R., Roberto, A. S., Zolia, N. G. and Luis, B. F. 2010. Diversity of endophytic fungi of Taxus globosa (Mexican yew). Fungal Div. 47:65-74.
  19. Huang, W. Y., Cai, Y. Z., Surveswaran, S., Hyde, K. D., Corke, H. and Sun, M. 2009. Molecular phylogenetic identification of endophytic fungi isolated from three Artemisia species. Fungal Div. 36:69-88.
  20. Huang, W. Y., Cai, Y. Z., Surveswaran, S., Hyde, K. D., Corke, H. and Sun, M. 2008. Biodiversity of endophytic fungi associated with 29traditional Chinese medicinal plants. Fungal Div. 33:61-75.
  21. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16:111-120. https://doi.org/10.1007/BF01731581
  22. Larran, S., Monaco, C. and Alippi, H. 2001. Endophytic fungi in leaves of Lycopersicon esculentum Mill. World J. Microbiol. Biotechnol. 17:181-184. https://doi.org/10.1023/A:1016670000288
  23. Larran, S., Perello, A., Simon, M. R. and Moreno, V. 2002. Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves. World J. Microbiol. Biotechnol. 18:683-686. https://doi.org/10.1023/A:1016857917950
  24. Lv, Y. L., Zhang, F. S., Chen, J., Cui, J. L., Xing, Y. M., Li, X. D. and Guo, S. X. 2010. Diversity and antifungal activity of endophytic fungi associated with the alpine plant Saussurea involucrata. Biol. Pharm. Bull. 33:1300-1306. https://doi.org/10.1248/bpb.33.1300
  25. Margherita, D., Salvatore, F. and Matteo, C. 2008. Endophytic fungi occurring in fennel, lettuce, chicory and celery-commercial crops in Southern Italy. Mycol. Res. 112:100-107. https://doi.org/10.1016/j.mycres.2007.11.007
  26. Murray, F. R., Latch, G. C. M. and Scott, D. B. 1992. Surrogate transformation of perennial rye grass, Lolium perenne, using genetically modified Acremonium endophyte. Mol. Genet. 233:1-9. https://doi.org/10.1007/BF00587554
  27. Narisawa, K., Kawamata, H., Currah, R. S. and Hashiba, T. 2002. Supperession of Verticillium wilt in eggplant by some fungal root endophytes. Eur. J. Plant Pathol. 108:103-109. https://doi.org/10.1023/A:1015080311041
  28. Park, J. H., Park, J. H., Choi, G. J., Lee, S. W., Jang, K. S., Choi, Y. H., Cho, K. Y. and Kim, J. C. 2003. Screening for antifungal endophytic fungi against six plant pathogenic fungi. Mycobiology 31:179-182. https://doi.org/10.4489/MYCO.2003.31.3.179
  29. Park, M. S., Seo, G. S., Bae, K. S. and Yu, S. H. 2005. Characterization of Trichoderma spp. Associated with green mold oyster mushroom by PCR-RFLP and sequence analysis of ITS regions of rDNA. Plant Pathol. J. 21:229-236. https://doi.org/10.5423/PPJ.2005.21.3.229
  30. Paul, N. C., Kim, W. K., Woo, S. K., Park, M. S. and Yu, S. H. 2007. Fungal endophytes in roots of Aralia species and their antifungal activity. Plant Pathol. J. 23:287-294. https://doi.org/10.5423/PPJ.2007.23.4.287
  31. Paul, N. C. 2007. Diversity of endophytic fungi of medicinal plants in Korea and their antifungal and plant growth promoting activity. Masters thesis, Chungnam National University, Daejeon, Republic of Korea.
  32. Perry, L., Dickau, R., Zarrillo, S., Holst, I., Pearsal, D. M., Piperno, D. R., Berman, M. J., Cooke, R. G., Rademaker, K., Ranere, A. J., Raymond, J. S., Sandweiss, D. H., Scaramelli, F., Tarble, K. and Zeidler, J. A. 2007. Starch fossils and the domestication and dispersal of chili peppers (Capsicum spp. L.) in the Americas. Science (Reports) 315:786-788.
  33. Petrini, O. and Carroll, G. C. 1981. Endophytic fungi in foliage on some Cupressaceae in Oregon. Can. J. Bot. 59:629-636. https://doi.org/10.1139/b81-089
  34. Rozin, P. and Schiller, D. 1980. The nature and acquisition of a preference for chili pepper by humans. Motiv. Emotion 4:77-101. https://doi.org/10.1007/BF00995932
  35. Rosa, L. H., Vieira, M. L. A., Santiago, I. F. and Rosa, C. A. 2010. Endophytic fungi community associated with the dicotyledonous plant Colobanthus quitensis (Kunth) Bartl. (Caryophyllaceae) in Antarctica. FEMS Microbiol. Ecol. DOI:10.1111/j.1574-6941.2010.00872.x.
  36. Rubini, M. R., Silva-Ribeiro, R. T., Pomella, A. W. V., Maki, C. S., Araujo, W. L., Santos, D. R. and Azevedo, J. L. 2005. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of witches broom disease. Int. J. Biol. Sci. 1:24-33.
  37. Sanchez, M. S., Bills, G. F. and Zabalgogeazcoa, I. 2008. Diversity and structure of the fungal endophytic assemblages from two sympatric coastal grasses. Fungal Div. 33:87-100.
  38. Schardl, C. L., Liu, J., White, J. K., Finkel, R. A., An, Z. and Siegel, M. 1991. Molecular phylogenetic relationship of non-pathogenic grass mycosymbionts and clavicipitaceous plant pathogens. Plant Syst. Evol. 178:27-41. https://doi.org/10.1007/BF00937980
  39. Sette, L. D., Passarini, M. R. Z., Delarmelina, C., Salati, F. and Duarte, M. C. T. 2006. Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World J. Microbiol. Biotechnol. 22:1185-1195. https://doi.org/10.1007/s11274-006-9160-2
  40. Shannon, C. E. and Weaver, W. 1949. The mathematical theory of communication. University of Illinois Press, Urbana, USA.
  41. Sieber, T., Riesen, T. K., Muller, E. and Fried, P. M. 1988. Endophytic fungi in four winter cultivars (Triticum aestivum L.) differing in resistance against Stagonospora nodorum (Berk.) Cast. And germ.= Septoria nodorum (Berk.) Berk. J. Phytopathol. 122:2289-2306.
  42. Simpson, E. H. 1949. Measurement of species diversity. Nature (London) 163:688. https://doi.org/10.1038/163688a0
  43. Spurr, H. W. Jr. and Welty, R. E. 1975. Characterization of endophytic fungi in healthy leaves of Nicotiana spp. Phytopathology 65:417-422. https://doi.org/10.1094/Phyto-65-417
  44. Strobel, G. and Daisy, B. 2003. Bioprocessing for microbial endophytes and their natural products. Microbiol Mol. Biol. Rev. 67:491-502. https://doi.org/10.1128/MMBR.67.4.491-502.2003
  45. Strobel, G. A., Hess, W. M., Ford, E., Sidhu, R. S. and Yang, X. 1996. Taxol from fungal endophytes and the tissue of biodiversity. J. Ind. Microbiol. 17:417-423. https://doi.org/10.1007/BF01574772
  46. Tanaka, A., Christensen, M. J., Takemoto, D., Park, P. and Scott, B. 2006. Reactive oxygen species play a role in regulating a fungus-perennial ryegrass mutualistic interaction. Plant Cell 18:1052-1066. https://doi.org/10.1105/tpc.105.039263
  47. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. 1997. ClustalX: windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:487-4878.
  48. Tudzynski, B. and Sharon, A. 2002. Biosynthesis, biological role and application of fungal phyto-hormones. In: Osiewacz of Pestalotiopsis isolated as endophytes from medicinal plants. Fungal Div. 24: 37-54.
  49. Yuan, Z. L., Zhang, C. L., Lin, F. C. and Kubicek, P. 2010. Identity, diversity, and molecular phylogeny of the endophytic mycobiota in the roots of rare wild rice (Oryza granulate) from the nature reserve in Yunnan, China. Appl. Environ. Microbiol. March 1642-1652.
  50. Wang, F. W., Jiao, R. H., Cheng, A. B., Tan, S. H. and Song, Y. C. 2007. Antimicrobial potentials of endophytic fungi residing in Quescus variabilis and brefeldin A obtained from Cladosporium sp. World J. Microbiol. Biotechnol. 23:79-83. https://doi.org/10.1007/s11274-006-9195-4
  51. White, T. J., Bruns, T. D., Lee, S. B. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal DNA for phylogenetics. In: PCR protocols: A guide to the methods and applications, pp. 315. Eds. MA Innes, DH Gelfand JJ, Sninsky and White TJ. Academic Press, New York.

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