Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Enhanced Pathogenicity of Bacillus thuringiensis Mixed with a Culture Broth of an Entomopathogenic Bacterium, Xenorhabdus sp.

제노랍두스 곤충병원세균 배양액의 비티 미생물 약제 약효증진 효과

  • Seo, Sam-Yeol (Department of Bioresource Sciences, Andong National University) ;
  • Ahn, Haet-Nim (Department of Bioresource Sciences, Andong National University) ;
  • Eom, Seong-Hyeon (Department of Bioresource Sciences, Andong National University) ;
  • Im, Eun-Yeong (Department of Bioresource Sciences, Andong National University) ;
  • Park, Ji-Young (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
  • 서삼열 (안동대학교 자연과학대학 생명자원과학과) ;
  • 안햇님 (안동대학교 자연과학대학 생명자원과학과) ;
  • 엄성현 (안동대학교 자연과학대학 생명자원과학과) ;
  • 임은영 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박지영 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2011.11.22
  • Accepted : 2012.01.25
  • Published : 2012.03.01
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Abstract

The entomopathogenic bacterium, $Xenorhabdus$ sp., was isolated from an entomopathogenic nematode, $Steinernema$ $monticolum$. When these bacteria were injected into the hemocoel of the diamondback moth, $Plutella$ $xylostella$, they caused significant mortality. However, the bacterium was not pathogenic when it was administered orally. This study showed that $Xenorhabdus$ sp. significantly enhanced oral pathogenicity of $Bacillus$ $thuringiensis$ (Bt) against the last instar larvae of $P.$ $xylostella$. Different ratios of culture broth of $Xenorhabdus$ sp. and Bt showed significantly different pathogenicities against $P.$ $xylostella$. In field tests, the optimal bacterial mixture significantly enhanced control efficacy against $P.$ $xylostella$ compared to Bt treatment alone. These results demonstrated that $Xenorhabdus$ sp. culture broth can be developed as a potent biopesticide by enhancing the insecticidal efficacy of Bt.

곤충병원세균인 제노랍두스($Xenorhabdus$ sp.)는 곤충병원선충인 $Steinernema$ $monticolum$으로부터 분리되었다. 이 세균 배양액을 배추좀나방($Plutella$ $xylostella$) 혈강에 주입할 경우 높은 병원력을 나타내지만, 섭식 처리할 경우 낮은 병원력을 보였다. 본 연구는 이 제노랍두스 세균 배양액이 $Bacillus$ $thuringiensis$(비티)와 혼합하여 배추좀나방 종령충에 처리할 경우 비티의 병원력을 뚜렷하게 증가시키는 것을 보였다. 또한 제노랍두스 배양액과 비티의 혼합비율을 달리할 경우 병원력이 크게 차이를 보였다. 최적의 두 세균 혼합비율을 이용하여 야외에 발생한 배추좀나방에 처리하였으며, 비티 단독처리에 비해 뚜렷이 상승된 방제 효과를 확인할 수 있었다. 이러한 결과는 제노랍두스 배양액을 비티와 혼합하여 새로운 미생물 살충제로 개발할 수 있는 가능성을 제시했다.

Keywords

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