The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effects of Achyranthis Radix Extract Mixed with Hydrogel on Osteoclast Differentiation

하이드로젤에 탑재한 우슬(牛膝)추출물의 효과적인 파골세포 분화 억제 작용

  • Choi, Jin-Young (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Chang-Hoon (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University) ;
  • Jang, Jun-Bock (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Kyung-Sub (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Jin-Moo (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University)
  • 최진영 (경희대학교 한의과대학 한방부인과 교실) ;
  • 이창훈 (경희대학교 한의과대학 한방부인과 교실) ;
  • 장준복 (경희대학교 한의과대학 한방부인과 교실) ;
  • 이경섭 (경희대학교 한의과대학 한방부인과 교실) ;
  • 이진무 (경희대학교 한의과대학 한방부인과 교실)
  • Received : 2012.01.26
  • Accepted : 2012.02.07
  • Published : 2012.02.24
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: This study was conducted to evaluate the inhibitory effect of Achyranthis Radix extract(ARE) loaded hydrogel on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of ARE, Achyranthis Radix-alginate hydrogel disk(ARHD) in bone marrow macrophages stimulated(BMMs) with human receptor activator of nuclear factor-${\kappa}B$ ligand(RANKL), human macrophage-colony stimulating factor(M-CSF). Tartrate resistant acid phosphatase staining and RT-PCR were performed to know the inhibitory effect on osteoclast differentiation. Reactive oxygen species and actin ring formation were analysed to observe the effect of ARHD. Results: ARE has no cytotoxicity at the concentration of 0.1 $mg/m{\ell}$ or lower. ARE decreased the number of TRAP positive cells in RANKL, M-CSF stimulated BMMs and the gene expression. ARHD has no cytotoxicity at the concentration of 10 ${\mu}g/m{\ell}$ (24, 48hours), 50 ${\mu}g/m{\ell}$ (24 hours). ARHD restrained the synthesis of reactive oxygen species and the formation of actin ring. Conclusions: Achyranthis Radix has the inhibitory effect of osteoclast differentiation and bone resorption. Further studies are needed to treat osteoporosis by Achyranthis Radix.

Keywords

References

  1. Rigs BL, Khosla S, Melton III LJ. Sex steroids and the construction and conservation of the adult skeleton. Endocr Rev. 2002;23(3):279-302. https://doi.org/10.1210/edrv.23.3.0465
  2. Manolagas SC. Brith and death of bone cells : Basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev. 2000;21:115-37.
  3. Steven LT. Bone resorption by osteoclasts. Science. 2000;289:1504-8. https://doi.org/10.1126/science.289.5484.1504
  4. Tamara A, Rik D. Interfering with bone remodelling. Nature. 2002;416:686-7. https://doi.org/10.1038/416686a
  5. William JB, Scott S, David LL. Osteoclast differentiation and activation. Nature. 2003;423:337-42. https://doi.org/10.1038/nature01658
  6. 김동균, 류지윤, 이언정. 腎主骨에 관한 東西醫學的 考察. 대한한방내과학회지. 1991;12(2):26-9.
  7. 김종환. 골다공증에 관한 문헌적 고찰-주로 최근의 한의학적 임상 및 실험 논문을 중심으로. 1998;15(2):437-54.
  8. 한의부인과학 교재 편찬위원회. 한의부인과학. 서울:정담출판사. 2002:230.
  9. 전국한의과대학 본초학교수 공편저. 본초학. 서울:영림사. 2000:427.
  10. 김주호 등. 牛膝의 파골세포 분화 억제와 골흡수 억제효과. 대한본초학회지. 2010;25(1):65-74.
  11. Rigs BL, Khosla S, Melton III LJ. A unitary model for involutional osteoporosis: estrogen deficiency causes both type I and type II osteoporosis in postmenopausal women and contributes to bone loss in aging men. J Bone Miner Res. 1998;13(5):763-73. https://doi.org/10.1359/jbmr.1998.13.5.763
  12. Takashi K et al. Estrogen inhibits bone resorption by directly inducing apoptosis if the bone-resorbing osteoclast. J Exp Med. 1997;4:489-95.
  13. Shevde NK et al. Estrogen suppress RANK ligand-induced osteoclast differentiation via a stromal cell independent mechanism involving c-jun repression. Natl Acad Sci USA. 2000;97(14):7829-34. https://doi.org/10.1073/pnas.130200197
  14. Writing group for the women's health initiative investigators. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: Principal results from the women's health initiative randomized controlled trial. JAMA. 2002;288(3):321-33. https://doi.org/10.1001/jama.288.3.321
  15. Nelson HD et al. Postmenopausal hormone replacement therapy : Scientific review. JAMA. 2002;288(7):872-81. https://doi.org/10.1001/jama.288.7.872
  16. Lacey DL et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell. 1998;93:165-76. https://doi.org/10.1016/S0092-8674(00)81569-X
  17. Hisataka Yasuda et al. Osteoclast differentiation factor is a ligand for osteoprotegerin / osteoclastogenesisinhibitory factor and is identical to TRANCE. RANKL. Natl Acad Sci USA. 1998;95:3597-602. https://doi.org/10.1073/pnas.95.7.3597
  18. 孫星衍, 孫馮翼. 神農本草經. 北京:科學技術出版社. 1999:13.
  19. 이영종, 박재상. 牛膝의 形態 감별에 관한 연구. 대한본초학회지. 2005;20(2):77-82.
  20. Shen H et al. Achyranthes bidentata polypeptides confer neuroprotection through inhibition of reactive oxygen species production, bax expression, and mitochondrial dysfunction induced by overstimulation of N-methyl-D-aspartate receptors. J Neurosci Res. 2010;88(3):669-76.
  21. Xiang DB, Li XY. Antitumor activity and immuno-potentiating actions of Achyranthes bidentata polysaccharides. Acta Pharmacol Sin. 1993;14(6):556-61.
  22. Sun HX. Adjuvant effect of Achyranthes bidentata saponins on specific antibody and cellular response to ovalbumin in mice. Vaccine. 2006;24(17):3432-9. https://doi.org/10.1016/j.vaccine.2006.02.014
  23. Park HJ et al. The Anti-nociceptive and anti-inflammatory effect of Achyranthes Japonica Nakai. Korean Journal of oriental medicine. 2004;25(4):8-14.
  24. 박재상, 성낙술, 이용종. 牛膝, 懷牛膝 및 川牛膝의 산화억제 효과 비교 연구. 대한본초학회지. 2007;22(4):155-67.
  25. 정선미 등. 牛膝추출물의 Clostridium difficile에 대한 항균 효과. 한국식품과학회지. 2007;39(5):564-8.
  26. 최기복 등. 류마티스 관절염 흰쥐에서 수중 운동과 牛膝추출물이 염증 및 통증 반응에 미치는 영향. 동의생리병리학회지. 2009;23(1):113-20.
  27. 김계엽 등. 류마티스 관절염에서 牛膝과 전침자극의 항관절염 효과. 동의생리병리학회지. 2007;21(2):425-31.
  28. 장통영 박희수. 牛膝약침(牛膝藥鍼)이 Rat의 Adjuvant 관절염(關節炎)에 미치는 영향(影響). 대한한의학회지. 2000;21(3):77-87.
  29. 서은아, 문형철. 牛膝 추출물이 골아세포 증식과 분화에 미치는 효과. 동의생리병리학회지. 2004;18(6):1821-7.
  30. 김은정 등. 골다공증 쥐에서 牛膝의 농도별 투여에 따른 골대사의 생화학적 마커에 미치는 영향. 동의생리병리학회지. 2007;21(4):967-72.
  31. He CC et al. Osteoprotective effect of extract from Achyranthes bidentata in ovariectomized rats. J Ethnopharmacol. 2010;127:229-34. https://doi.org/10.1016/j.jep.2009.11.016
  32. 이효정 등. 천연물 추출물의 파골세포 분화억제 효과 검색. 한국식품과학회지. 2005;37(6):997-1004.
  33. 이준원. 생약의 파골 세포 분화 저해 활성 검색. 생약학회지. 2009;40(2):83-8.
  34. Minkin C. Bone acid phosphatase: Tartrate-resistant acid phosphatase as a marker of osteoclast function. Calcif Tissue Int. 1982;34(3):285-90. https://doi.org/10.1007/BF02411252
  35. Jussi MH et al. Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase. J Biol Chem. 1999;274(33):22907-10. https://doi.org/10.1074/jbc.274.33.22907
  36. Takuma M et al. Critical role of cortactin in actin ring formation and osteoclastic bone resorption. J Bone Miner Metab. 2006;24:368-72. https://doi.org/10.1007/s00774-006-0701-4

Cited by

  1. 하고초(夏枯草)에서 추출한 Ursolic acid의 파골세포 분화 억제 효과 vol.27, pp.2, 2012, https://doi.org/10.15204/jkobgy.2014.27.2.059