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T Cell Immunoglobulin Mucin Domain (TIM)-3 Promoter Activity in a Human Mast Cell Line

  • Kim, Jung Sik (Department of Microbiology, Ajou University School of Medicine) ;
  • Shin, Dong-Chul (Department of Microbiology, Ajou University School of Medicine) ;
  • Woo, Min-Yeong (Department of Microbiology, Ajou University School of Medicine) ;
  • Kwon, Myung-Hee (Department of Microbiology, Ajou University School of Medicine) ;
  • Kim, Kyongmin (Department of Microbiology, Ajou University School of Medicine) ;
  • Park, Sun (Department of Microbiology, Ajou University School of Medicine)
  • Received : 2012.09.04
  • Accepted : 2012.09.21
  • Published : 2012.10.31
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Abstract

T cell immunoglobulin mucin domain (TIM)-3 is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Therefore, we examined the transcript level and the promoter activity of TIM-3 in mast cells. The TIM-3 transcript level was assessed by real-time RT-PCR and promoter activity by luciferase reporter assay. TIM-3 mRNA levels were increased in HMC-1, a human mast cell line by TGF-${\beta}1$ stimulation but not by stimulation with interferon (IFN)-${\alpha}$, IFN-${\lambda}$, TNF-${\alpha}$, or IL-10. TIM-3 promoter -349~+144 bp region relative to the transcription start site was crucial for the basal and TGF-${\beta}1$-induced TIM-3 promoter activities in HMC-1 cells. TIM-3 promoter activity was increased by over-expression of Smad2 and Smad4, downstream molecules of TGF-${\beta}1$ signaling. Our results localize TIM-3 promoter activity to the region spanning -349 to ＋144 bp in resting and TGF-${\beta}1$ stimulated mast cells.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea(NRF)

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