생명과학회지 (Journal of Life Science)

  • 제22권4호
  • /
  • Pages.466-471
  • /
  • 2012
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

RAPD 마커에 의한 한국, 중국, 일본 참가리비의 유전적 다양성과 집단 구조

Genetic Diversity and Population Structure of the Scallop Patinopecten yessoensis in Korea, China, and Japan by Random Amplified Polymorphic DNA Markers

  • Nam, Myung-Mo (Aquaculture Industry Division, East Sea Fisheries Research Institute) ;
  • Lee, Chu (Aquaculture Industry Division, East Sea Fisheries Research Institute) ;
  • Moon, Tae-Seok (Aquaculture Industry Division, East Sea Fisheries Research Institute) ;
  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
  • 투고 : 2012.01.31
  • 심사 : 2012.03.15
  • 발행 : 2012.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

참가리비($Patinopecten$ $yessoensis$) 60개체를 채집하여 유전적 다양성과 집단구조를 조사하였다. 임의 유전 다형성 DNA (RAPD)로 109개 유전자형과 79개 다형성 좌위(72.8%)를 발견하였다. 전체 유전적 다양도($H_T$)와 집단내 변이($H_S$)는 각각 0.254와 0.178였다. 유전자 좌위에 근거하여 집단 간 분화 정도($G_{ST}$)는 0.299였다. 이는 전체변이의 약 70.1%가 집단 내에 존재하고 있음을 시사한다. 참가리비 세 나라 집단에서 한 집단에 국한되는 대립유전자 좌위와 한 개체에만 발현된 밴드가 발견되었다. RAPD 마커는 한국, 중국, 일본에 분포하는 참가리비를 구분하는데 매우 효과적이었다. 또한 참가리비의 집단 내, 집단 간 유전적 다양성에 대한 통찰은 동물 유전자원의 수진 전략과 양식에 유익할 것으로 사료된다.

Sixty individuals of the scallop $Patinopecten$ $yessoensis$ (Genus Pecten) were sampled to examine the genetic diversity and population structure of this species. Random amplified polymorphic DNA (RAPD) identified 109 genotypes and produced 79 polymorphic loci (72.8%). Total genetic diversity values ($H_T$) and interlocus variation in the within-population genetic diversity ($H_S$) were 0.254 and 0.178, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) was 0.299. This indicated that about 70.1% of the total variation was within populations. The unique loci and bands of $P.$ $yessoensis$ were shown in only one population among the three countries. RAPD markers were very effective in classifying the natural population levels of $P.$ $yessoensis$ in Korea, China, and Japan. In addition, insights into the relative gene diversity among and within populations of $P.$ $yessoensis$ would be useful in breeding and for the development of strategies for animal genetic resources.

키워드

참고문헌

  1. Brand, A. R. 1991. Scallop ecology: distribution and behavior, pp. 517-584, In Shumway, S. E. (ed.), Scallops: Biology, Ecology and Aquaculture, Elsevier, Amsterdam.
  2. Caddy, J. F. 1970. A method of surveying scallop populations from a submersible. J. Fish. Res. Bd Can. 27, 535-549. https://doi.org/10.1139/f70-057
  3. Doebley, J. 1989. Isozymic evidence and the evolution of crop plants, pp. 46-72, In Soltis, D. E. and P.S. Soltis (eds.), Isozymes in Plant Biology, Dioscorides Press, Portland.
  4. Fisheries Global Information System. 2010. Food and Agriculture Organization of the United Nations.
  5. Goshima, S. and Fujiwara, H. 1994. Distribution and abundance of cultured scallop Patinopecten yessoensis in extensive sea beds as assessed by underwater camera. Mar. Ecol. Prog. Ser. 110, 151-158. https://doi.org/10.3354/meps110151
  6. Hedrick, P. W. 1998. Maintenance of genetic polymorphism: spatial selection and self-fertilization. Am. Nat. 152, 145-150. https://doi.org/10.1086/286155
  7. Ito, H. 1991. Patinopecten (Mizuhopecten) yessoensis, pp. 1024-1055, In Shumway, S. E. (ed.), Scallops: Biology, Ecology and Aquaculture, Elsevier, Amsterdam.
  8. Li, Q., K. Xu, and R. I. Yu. 2007. Genetic variation in Chinese hatchery populations of the Japanese scallop (Patinopecten yessoensis) inferred from microsatellite data. Aquaculture 269, 211-219. https://doi.org/10.1016/j.aquaculture.2007.04.017
  9. MacDonald, B. A. and Bajdik, C. D. 1992. Orientation and distribution of individual Placopecten magellanicus (Gmelin) in two natural populations with differing production. Can. J. Fish. Aquat. Sci. 49, 2086-2092. https://doi.org/10.1139/f92-232
  10. Martinez, G., Mettifogo, L., Perez, M. A. and Callejas, C. 2007. A method to eliminate self-fertilization in a simultaneous hermaphrodite scallop. 1. Effects on growth and survival of larvae and juveniles. Aquaculture 273, 459-469. https://doi.org/10.1016/j.aquaculture.2007.10.033
  11. Molnar, S. J., James, L. E. and Kash, .K. J. 2000. Inheritance and RAPD tagging of multiple genes for resistance to net blotch in barley. Genome 43, 224-231. https://doi.org/10.1139/g99-111
  12. Olsen, A. M. 1955. Underwater studies on the Tasmanian commercial scallop, Notovola mendionalis (Tate) (Lamel libranchiata: Pectinidae). Aust. J. mar. Freshwat. Res. 6, 392-409. https://doi.org/10.1071/MF9550392
  13. Pacak, A., Fiedorow, P., Dabert, J. and Szweykowska-kulinska, Z. 2010. RAPD technique for taxonomic studies of Pellia epiphylla-complex (Hepaticae, Metzgeriales). Genetica. 104, 179-187.
  14. Saitou, N. and Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
  15. Sato, M., Kawamata, K., Zaslavskaya, N., Nakamura, A., Ohta, T., Nishikiori, T., Brykov, V. and Nagashima, K. 2005. Development of microsatellite markers for Japanese scallop (Mizuhopecten yessoensis) and their application to a population genetic study. Marine Biotech. 7, 713-728. https://doi.org/10.1007/s10126-004-0127-8
  16. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731-2739. https://doi.org/10.1093/molbev/msr121
  17. Van Treuren, R., Bijlsma, R., van Delden, W. and Ouborg, N. J. 1991. The significance of genetic erosion in the process of extinction. I. Genetic differentiation in Salvia pratensis and Scabiosa columbaria in relation to population size. Heredity 66, 181-189. https://doi.org/10.1038/hdy.1991.23
  18. Wang, Q. C. 1984. Introduction of Japanese scallop and prospect of culture it in northern China. Fish. Sci. 3, 24-27.
  19. Yeh, F. C., Yang, R. C., Botle, T. J., Ye, Z. H. and Mao, J. X. 1997. POPGENE, the user friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Canada.

피인용 문헌

  1. Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF-7 human breast carcinoma cells vol.14, pp.2, 2017, https://doi.org/10.3892/ol.2017.6424