생약학회지 (Korean Journal of Pharmacognosy)

  • 제43권1호
  • /
  • Pages.27-31
  • /
  • 2012
  • /
  • 0253-3073(pISSN)
  • /
  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

엉겅퀴 뿌리 및 꽃 추출물의 간 성상세포 활성 억제 효과

Inhibitory Effect of Cirsium japonicum Root or Flower Extract on Hepatic Stellate Cells Activation

  • 투고 : 2012.01.27
  • 심사 : 2012.03.06
  • 발행 : 2012.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was designed to elucidate the effects of Cirsium japonicum (CJ) extracts on hepatic stellate cells (HSCs, LX-2 cells) proliferation, which is induced by platelet-derived growth factor (PDGF) or transforming growth factor-${\beta}$ (TGF-${\beta}$). The content of total phenol, flavonoid, and silymarin derivatives was more higher in CJ-flower than in CJ-root. Consistent with these results, the LX-2 cells growth inhibition was more effective in CJ-flower extract than in CJ-root extract, the complete growth inhibition concentration was $1{\mu}g/mL$ and $50{\mu}g/mL$, respectively. These results suggest that extracts from CJ-flower can be potentially used as therapeutic substances for the regulatioin of HSCs activation.

키워드

참고문헌

  1. Lee, S. J. (1996) Korean Folk Medicine, 99, 145-146, Seoul National University Press Seoul.
  2. Ishida, H., Umino, T., Tsuji, K. and Kosuge, T. (1987) Studies on antihemmorhagic substances in Herbs classified as hemostatics in Chinese medicine. VII. On the anthihmorrhagic principle in Cirsium japonicum DC. Chem. Pharm. Bull. 35: 861-864. https://doi.org/10.1248/cpb.35.861
  3. Chung, M. S., Um, H. J., Kim, C. K. and Kim, G. H. (2007) Development of functional tea product using Circium japoinucum. Korean J. Food Culture 22: 261-265.
  4. Park, J. C., Hur, J. M., Park J. G., Kim S. C., Park, I. R., Choi, S. H. and Choi, J. W. (2004) Effects of methanol extract of Cirsium japonicum var. ussurense and its principle, hipidulin- 7-O-neohesperidoside on hepatic alchohol-metabolizing enzymes and lipid peroxidation in ethanol-treated rats. Phytother. Res. 18: 19-24. https://doi.org/10.1002/ptr.1299
  5. Lim, S. S. and Lee, J. H. (1997) Effect of Artemisia princeps var. orientalis and Cicium japonicum var. ussurense on serum lipid of hyperlipidemic rat. Korean Nutr. Soc. 30: 12-18.
  6. Tsukada, S., Parosns, C. J. and Ripe, R. A. (2006) Mechanism of liver fibrosis. Clin. Chim. Acta. 364: 33-60. https://doi.org/10.1016/j.cca.2005.06.014
  7. Winau, f., Hegasy, G. and Weiskirchen, R. (2007) Ito cells are liver-residnet antigen-presenting cells for activating T cell responses. Immunity 26: 117-129. https://doi.org/10.1016/j.immuni.2006.11.011
  8. Friedman, S. L., Roll, J. F., Boyles, J. and Bissell, D. M. (1985) Hepatic lipocytes. the principal collagen producing cells of normal rat liver. Proc. Natl. Acad. Sci. USA. 82: 8681-8685. https://doi.org/10.1073/pnas.82.24.8681
  9. Bedossa, P., Houglum, K., Trautwein, C., Holstege, A. and Chojkier, M. (1994) Stimulation of collagen a1(I) gene expression is associated with lipid peroxidation in hepatocellular injury: a link to tissue fibrosis. Hepatology 19: 1262- 1271.
  10. Pinzani, M. Hepatic stellate (Ito) cells: expanding roles for a liver specific pericyte. (1995) J. Hepatol. 22: 700-706.
  11. Moreno, M. I. N., Isla, M. I. N., Sampietro, A. R. and Vatuone, M. A. (2000) Comparison of the free radical-scavenging activity of propolis from several region of Argentina. J. Ethnopharmacol. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  12. Friedman, S. L. (1996) Hepatic stellate cells. Prog. Liver Dis. 14: 101-130.
  13. Otto, F. and Denis, W. (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-243.
  14. Moreno, M. I. N., Isla, M. I. N., Sampietro, A. R. and Vattuone, M. A. (2000) Comparison of the free radical-scavenging activity of propolis from several region of Argentina. J. Ethnopharmacol. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  15. Liu, H., Du, Z. and Yuan, Q. (2009) A novel rapid method for simultaneous determination of eight active compounds in silymarin using reversed-phased UPLC-UV detector. J. Chromatogr. B. 877: 4159-4163. https://doi.org/10.1016/j.jchromb.2009.11.001
  16. Vogel, G., Trost, W. and Braatz, R. (1975) Studies on the pharmacodynamics, including site and mode of action of silymarin: The antihepatotoxic principle from Silybum mar (L) Gaertn. Arzeiittelforsch 25: 2-9.