Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biocontrol of Anthracnose in Pepper Using Chitinase, ${\beta}$-1,3 Glucanase, and 2-Furancarboxaldehyde Produced by Streptomyces cavourensis SY224

  • Lee, So Youn (Department of Agricultural Chemistry, Chonnam National University) ;
  • Tindwa, Hamisi (Department of Agricultural Chemistry, Chonnam National University) ;
  • Lee, Yong Seong (Department of Agricultural Chemistry, Chonnam National University) ;
  • Naing, Kyaw Wai (Department of Agricultural Chemistry, Chonnam National University) ;
  • Hong, Seong Hyun (Department of Agricultural Chemistry, Chonnam National University) ;
  • Nam, Yi (Ansung Training Institute, National Agricultural Cooperative Federation) ;
  • Kim, Kil Yong (Department of Agricultural Chemistry, Chonnam National University)
  • Received : 2012.03.05
  • Accepted : 2012.05.22
  • Published : 2012.10.28
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Abstract

A strain of Streptomyces cavourensis subsp. cavourensis (coded as SY224) antagonistic to Colletotrichum gloeosporioides infecting pepper plants was isolated. SY224 produced lytic enzymes such as chitinase, ${\beta}$-1,3-glucanase, lipase, and protease in respective assays. To examine for antifungal activity, the treatments amended with the nonsterilized supernatant resulted in the highest growth inhibition rate of about 92.9% and 87.4% at concentrations of 30% and 10%, respectively. However, the sterilized treatments (autoclaved or chloroform treated) gave a lowered but significant inhibitory effect of about 63.4% and 62.6% for the 10% supernatant concentration, and 75.2% and 74.8% for the of 30% supernatant concentration in the PDA agar medium, respectively, indicative of the role of a non-protein, heat stable compound on the overall effect. This antifungal compound, which inhibited spore germination and altered hyphal morphology, was extracted by EtOAc and purified by ODS, silica gel, Sephadex LH-20 column, and HPLC, where an active fraction was confirmed to be 2-furancarboxaldehyde by GS-CI MS techniques. These results suggested that SY224 had a high potential in the biocontrol of anthracnose in pepper, mainly due to a combined effect of lytic enzymes and a non-protein, heat-stable antifungal compound, 2-furancarboxaldehyde.

Keywords

References

  1. Al-Anbagi, R. A. and Fakhir E. Hameed. 2006. Effect of furfural on the germination and production of sclerotia of Sclerotinia sclerotiorum. AL-fath J. 25.
  2. Bartnicki-Garcia, S. 1968. Cell wall chemistry, morphogenesis, and taxonomy of fungi. Ann. Rev. Microbiol. 22: 87-108. https://doi.org/10.1146/annurev.mi.22.100168.000511
  3. Becker, J. O. 1993. Control of soil-born pathogens with living bacteria and fungi: Status and outlook. Pesticide Sci. 37: 355-363. https://doi.org/10.1002/ps.2780370408
  4. Chun, J. and M. Goodfellow. 1995. A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 45: 240-245. https://doi.org/10.1099/00207713-45-2-240
  5. Dai, D. H., W. L. Hu, G. R. Huang, and W. Li. 2011. Purification and characterization of a novel extracellular chitinase from thermophilic Bacillus sp. Hu1. Afr. J. Biotechnol. 10: 2476-2485.
  6. Fiedler, H. P., S. Worner, H. Zahner, H. P. Kaiser, W. K. Schierlein, and A. Muller. 1981. Metabolic products of microorganisms: Isolation and characterization of niphithricins A, B, and elaiophylin, antibiotics produced by Streptomyces violaceoniger. J. Antibiotics 34: 1107-1118. https://doi.org/10.7164/antibiotics.34.1107
  7. Forman, L. B., J. Postma, and J. A. Van Veen. 2003. Characterisation of Lysobacter enzymogenes (Christensen and Cook 1978) strain 3.1T8, a powerful antagonist of fungal disease of cucumber. Microbiol. Res. 158: 107-115. https://doi.org/10.1078/0944-5013-00185
  8. Getha, K. and S. Vikineswary. 2002. Antagonistic effects of Streptomyces violaceusniger strain G10 on Fusarium oxysporum f.sp. cubense race 4: Indirect evidence for the role of antibiosis in the antagonistic process. J. Ind. Microbiol. Biotechnol. 28: 303-310. https://doi.org/10.1038/sj.jim.7000247
  9. Handelsman, J. and E. V. Stabb. 1996. Biocontrol of soilborne plant pathogen. Plant Cell 8: 1855-1869.
  10. Holtzel, A., C. Kempter, J. G. Metzger, G. Jung, I. Groth, T. Fritz, and H. P. Fiedler. 1998. Spirofungin, a new antifungal antibiotic from Streptomyces violaceusniger Tu 4113. J. Antibiotics 51: 699-707. https://doi.org/10.7164/antibiotics.51.699
  11. Horvath, I. S., C. J. Franzen, J. Mohammad, M. J. Taherzadeh, C. Niklasson, and G. Liden, 2003. Effects of furfural on the respiratory metabolism of Saccharomyces cerevisiae in glucoselimited chemostats. Appl. Environ. Microbiol. 4076-4086.
  12. Joo, G. J. 2005. Production of an anti-fungal substance for biological control of Phytophthora capsici causing Phytophthora blight in red-peppers by Streptomyces halstedii. Biotechnol. Lett. 27: 201-205. https://doi.org/10.1007/s10529-004-7879-0
  13. Klose, S. and M. A. Tabatabai. 2002. Response of amidohydrolases in soils to chloroform fumigation. J. Plant Nutr. Soil Sci. 165: 125-132. https://doi.org/10.1002/1522-2624(200204)165:2<125::AID-JPLN125>3.0.CO;2-P
  14. Klose, S. and M. A. Tabatabai. 1998. Urease activity of microbial biomass in soils. Soil Biol. Biochem. 31: 205-211.
  15. Ko, H. S., R. D. Jin, H. B. Krishnan, S. B. Lee, and K. Y. Kim. 2009. Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydrocyphenylacetic acid and several lytic enzymes. Curr. Microbiol. 59: 608-615. https://doi.org/10.1007/s00284-009-9481-0
  16. Ko, H. S., H. Tindwa, R. D. Jin, Y. S. Lee, S. H. Hong, H. N. Hyun, et al. 2011. Investigation of siderophore production and antifungal activity against Phytophthora capsici as related to iron (iii) nitrition by Lysobacter antibioticus HS124. Korean J. Soil Sci. Fertilizer 44: 650-656.
  17. Kokubun, T., J. B. Hrborne, J. Eagles, and P. G. Waterman. 1995. Dibenzofuran phytoalexins from the sapwood of Cotoneaster acutifolius and five related species. Phytochemistry 38: 57-60. https://doi.org/10.1016/0031-9422(94)00636-8
  18. Kwon, C. S. and S. G. Lee. 2002. Occurrence and ecological characterization of red pepper anthracnose. Res. Plant Dis. 8: 120-123. https://doi.org/10.5423/RPD.2002.8.2.120
  19. Lazarova, G., V. Kostov, S. Zhelev, V. Ivanova, and T. Sokoloov. 1990. The effect of acetic acid and furfural on yeast growth. Acta Microbiol. Bulg. 25: 24-30.
  20. Lingappa, Y. and J. L. Lockwood. 1962. Chitin media for selected isolation and culture of actinomycetes. Phytopathology 52: 317-323.
  21. Lloyd, A. B., R. L. Noveroske, and J. L. Lockwood. 1965. Lysis of fungal mycelium by Streptomyces spp. and their chitinase systems. Phytopathology 55: 871-875.
  22. Mahadevan, B. and D. L. Crawford. 1997. Properties of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC108. Enzyme Microb. Technol. 20: 489-493. https://doi.org/10.1016/S0141-0229(96)00175-5
  23. Mayerhofer, H. J., R. T. Marshall, C. H. White, and M. Lu. 1972. Characterization of a heat-stable protease of Pseudomonas fluorescens P26. Appl. Microbiol. 25: 44-48.
  24. Obagwu, J. and L. Korsten. 2003. Intergrated control of citrus green and blue molds using Bacillus subtilis in combination with sodium bicarbonate of hot water. Postharvest Biol. Technol. 28: 187-194. https://doi.org/10.1016/S0925-5214(02)00145-X
  25. Palaniyandi, S. A., S. H. Yang, J. H. Cheng, L. Meng, and J. W. Suh. 2011. Biological control of anthracnose (Colletotrichum gloeosporioides) in yam by Streptomyces sp. MJM5763. J. Appl. Microbiol. 111: 443-455. https://doi.org/10.1111/j.1365-2672.2011.05048.x
  26. Park, K. S. and C. H. Kim. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean J. Plant Pathol. 8: 61-69.
  27. Prapagdee, B., C. Kuekulvong, and S. Mongkolsuk. 2008. Antifungal potential of extracellular metabolites produced by Streptomyces hygroscopicus against phytopathogenic fungi. Int. J. Biol. Sci. 4: 330-337.
  28. Sang, M. K., S. Chun, and K. D. Kim. 2008. Biological control of Phytophthora blight of pepper by antagonistic rhizobacteria selected from a sequential screening procedure. Biol. Control 46: 424-433. https://doi.org/10.1016/j.biocontrol.2008.03.017
  29. Soares, A. C. F., C. S. Sousa, M. S. Garrido, J. O. Perez, and N. S. de Almeida. 2006. Soil streptomycetes with in vitro activity against the yam pathogens Curvularia eragrostides and Colletotrichum gloeosporioides. Brazilian J. Microbiol. 37: 456-461. https://doi.org/10.1590/S1517-83822006000400010
  30. Trejo-Estrada, S. R., I. R. Sepulveda, and D. L. Crawford. 1998. In vitro and in vivo antagonism of Streptomyces violaceusniger YCED9 against fungal pathogens of turfgrass. World J. Microbiol. Biotechnol. 14: 865-872. https://doi.org/10.1023/A:1008877224089
  31. Yedidia, I., N. Benhamou, Y. Kapulnik, and I. Chet. 2000. Induction and accumulation of PR proteins activity during early stages of root colonization by the mycoparasite Trichoderma harzianum strain T-203. Plant Physiol. Biochem. 38: 863-873. https://doi.org/10.1016/S0981-9428(00)01198-0
  32. Yoon, G. Y., Y. S. Lee, S. Y. Lee, R. D. Park, H. N. Hyun, Y. Nam, and K. Y. Kim. 2012. Effects on Meloidogyne incognita of chitinase, glucanase and a secondary metabolite from Streptomyces cacaoi GY525. Nematology 14: 175-184. https://doi.org/10.1163/138855411X584124
  33. Zivkovi , S., S. Stojanovi , Z. Ivanovi , V. Gavrilovi , T. Popovi , and J. Balaz. 2010. Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides. Arch. Biol. Sci. Belgrade 62: 611-623. https://doi.org/10.2298/ABS1003611Z

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