Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Neuroprotective effects of Sohaphwangwon essential oil in a Parkinson's disease mouse model

MPTP로 유도된 Parkinson's disease 동물 모델을 이용한 소합향원(蘇合香元)의 신경보호 효과 및 그 작용 기전 연구

  • Kim, In-Ja (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Lee, Ji-Hyun (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Song, Kyoo-Ju (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Koo, Byung-Soo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University) ;
  • Kim, Geun-Woo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University)
  • 김인자 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 이지현 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 송규주 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 구병수 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 김근우 (동국대학교 한의과대학 한방신경정신과학교실)
  • Received : 2012.03.05
  • Accepted : 2012.03.15
  • Published : 2012.03.30
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Abstract

Objectives : To evaluate the neuroprotective effects of the essential oil from Sohaphwangwon (SH), a Chinese traditional medicinal prescription in a Parkinson's disease mouse model. Methods : 1. The neuroprotective effect of SH on primary neuronal cells was examined by using 1-methyl-4-phenylpyridinium ion (MPP+). 2. The neuroprotective effect of SH was examined in a Parkinson's disease mouse model. C57BL/6 mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg/day), intraperitoneal (i.p.) for 5 days. SH inhalation was applied before MPTP treatment for 7 days and continued until 12 days after the first MPTP treatment. 3. To find out the intracellular target signal molecule(s) regarding the neuroprotective effect of SH essential oil, brain-derived neurotropic factor (BDNF) and synaptic protein SNAP25 were examined by Western blot analysis. Results : 1. MPP+ induced a concentration-dependent decrease in cell viability. However, in the presence of 3 and 5 ug/ml of SH, MPP+-induced cell death was significantly reduced. 2. SH inhalation in MPTP mice led to the restoration of behavioral impairment and rescued tyrosine hydroxylase (TH)-positive dopaminergic neurodegeneration. 3. In SH / MPTP mice, BDNF and SNAP25 increased. Conclusions : This experiment suggests that the neuroprotective effect of SH essential oil is mediated by the expression of BDNF. Furthermore, SH essential oil may serve as a potential preventive or therapeutic agent regarding Parkinson's disease.

Keywords

References

  1. Dae Hee Lee. What is parkisonism? 4th ed. prb Pommunsa. p.2-4. 2005.
  2. Javoy-Agid, F. and Agid, Y. : Is the mesocortical dopaminergic system involved in Parkinson's disease?, Neurology 30, p.1326-1330, 1980. https://doi.org/10.1212/WNL.30.12.1326
  3. Sourkes, T. : Disorders of basal ganglia. In Basic Neurochemistry, 4th. New york, Eds, Ravan Press. p.811, 1989.
  4. Snyder, S. H. and D'Amato, R. J. : MPTP. A neurotoxin relevant to the pathophysiology of Parkinson's disease. Neurology 36, p.250-258. 1986. https://doi.org/10.1212/WNL.36.2.250
  5. Kojima, S., Matsuki, O. T., Nomura, T., Yamaoka, M. T. and Niki, E. : Elevation of oxidant potency in the brain of mice by low dose v-ray irradiation and its effect on 1-meyhyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) induced brain damage. Free radical Biology & Medicine 26, p.388-395. 1999. https://doi.org/10.1016/S0891-5849(98)00200-7
  6. Sriram , K ., Pai, K. s., Boyd , M. R . and Ravindranath, V. : Evidence for generation of oxidative stress in brain by MPTP: in vitro and in vivo studies in mice. Brain Research 749, p.44-52. 1997. https://doi.org/10.1016/S0006-8993(96)01271-1
  7. Wong, s. s., Li, R. H. Y., and Stadlin, A. : Oxidative stress induced by MPTP and $MPP^{+}$ : selective nulherability of cultured astrocytes. Brain Research 836, p.237-244. 1999. https://doi.org/10.1016/S0006-8993(99)01661-3
  8. Song-gil Min. Modern psychiatry. 5th ed. Seoul: Ilchogak; 2008. p. 240.
  9. Korean NeuroPsychiatric Association. Textbook of Neuropsychiatry. 2nd ed. Seoul: Chungang Munhwasa; 2005. p. 313.
  10. Hyung Chul Lee, Dae Geun Kim, Won Jun Jo, Seok Yeon Hwang, Young Gu Lee, Myung Dong Kim, et al. Protective Effect of R. palmatum on 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Neurotoxicity in Mice. Yakhak Hoeji 2002; 46:433-440.
  11. Sam June Lee. Effect of exercise on the expression of GDNF and 5-HT cells in the MPTP-induced Parkinson's disease for aging C57BL/6 mice. Sports science 2008; 17(3): 373-381.
  12. Ho Gun Hwa Kongjaksil Pyonjo. Ho Gun Hwa Haksul Kyonghom Hilyoung. Sanghae: Sanghae Chunguiyak Taehak Chulpansa; 2005. p. 31.
  13. Sandong Chunguihakwon Habuk Uihakwon Kyosuk. Hwangjenaegyong Somun. Kyosuk. Pukkyong: Inmin Wisaeng Chulpansa; 1982.
  14. Wang Geungdang. Sago Uihak Chongseo Zhungchi zhunseung. Sanghae: Sanghae Gojuk Chulpansa; 1991. p. 767-273.
  15. Zhu Munchun. Zhungui Nonyunbyung. Pukkyong: Pukkyong Chulpansa; 1995. p. 746-754
  16. Zhin Samun. Sago Uihak Chongseo, Taepyong Hyemin Hwaje Gukbang. 3th. vol. Sanghae: Sanghae Gojuk Chulpansa; 1991. p. 741-521.
  17. Zhinwi. Bangchehak. Sanghae: Sanghae Chunguihakwon Chulpansa; 1990. p. 283.
  18. Seobhyunchi. Chunguihak. Sanghae: Sanghae Chunguihakwon Chulpansa; 1988. p. 285.
  19. Byung Soo Koo, Keun Woo Kim, Seung Kyoon Ju. Jeon-gan Chiryo Yeongheombang. Seoul: Chongdam; 2003. p. 136-138.
  20. Chun Ho. Tongui pogam. Seoul: Namsandang; 1987. p. 93.
  21. Won Hee Kim, Gwang Joong Kim. Formation and System of Korean Medicine. Daegu: Chungmun Chulpansa; 1990. p. 78-84, 183-192.
  22. Tae Hwan Yeom. Dongui Chobang Daejeon. Seoul: Haengnimsowon; 1975. p. 73.
  23. Myong Shin Joo. Uimun pogam. Seoul: Haengnimsowon; 1975. p. 332.
  24. Do Yeon Hwang. Pangyak Happyon. Seoul: Namsandang; 1980. p. 204-205.
  25. Jae Gyom Shin. Pangyak Happyon Haesol. Seoul: Chontong Uihak Yonguso; 1988. p. 146-147.
  26. Gong Junghyun. Manbyong huichun. Pukkyong: Inmin Wisaeng Chulpansa; 1988. p. 67.
  27. Gidae Kim, Dae Kyoo Chung. An Experimental Study on the Change of Stress-related hormone Contents by Prescription of Kyogamdan and Sohaphyangwon. J. of Oriental Neuropsychiatry 1993;4(1):121-133.
  28. Soon Ju Kwen, Dae Kyoo Chung, Yun Sub Kim. A Study on Effect of Soeuminsohabhyangwon on the Brain Serotonin contents of Stressed Mice. J. of Oriental Neuropsychiatry 1998;9(2):87-95.
  29. Sang Gyung Nam, Kyung Sup Lee. Experimental studies on the effect of Woohwangchungsimwon and Sohaphyangwon. K.H.M. 1990;6(2):220-237.
  30. Ji Hyouck Kim, Wei Wan Whang. An Experimental Study on the Anti-Psychological Stress Effect by Sohaphyangwon. K.H.M. 1990;6(4):470-479.
  31. Jin Sang Jeon, Il Byung Song. Experimental Studies on the Effects of Soeuminohaphangwon. The journal of K.O.M.S. 1986;7(1):44-56.
  32. Yong hyun Shin. Effects of the Gagamsohaphyangwon Fragrance and Ganbodan on Central Nerve Depression and Receptor Activation in Mice. College of Oriental Medicine Graduate School of Dongguk University. 2000.
  33. Koo BS, Lee SI, Ha JH, Lee DU, Inhibitory effects of the essential oil from SuHeXiangWan on the central nervous system after inhalation. Bio.Pharm. Bull 27(4) p.515-519. 2004. https://doi.org/10.1248/bpb.27.515
  34. Su-Young Chon. Study on the Mechanism of Anticonvulsant Effect of Gagamsohaphangwon Fragrance upon Inhalation. College of Oriental Medicine Graduate School of Dongguk University. 2001.
  35. Seong-Soo Kim. Analgesic Effect upon Inhalation and Sedative Effect after Oral Administration of the Fragrance Oil of GagamSohaphyangwon. College of Oriental Medicine Graduate School of Dongguk University. 2002.
  36. Sung-Wook Kim. Effects of GagamSohaphwangwon essential oil on Glioma cells and Primary glial cell. College of Oriental Medicine Graduate School of Dongguk University. 2006.
  37. Ihn, Lee. Study on protection against $\beta$-amyloid peptide toxicity in vivo with inhalation of the essential oil extracted from GagamSohaphwangwon (GGSH). College of Oriental Medicine Graduate School of Dongguk University. 2007.
  38. Kim Seung-Tae. The neuroprotective effect of acupuncture at GB34 in a MPTP-induced Parkinson's disease mouse model. College of Oriental Medicine Graduate School of KyungHee University. 2009.
  39. Jin Hoon Lee. Neuroprotective effects of the acupoints Houxi(SI3) and Shenmai(BL62) on MPTPinduced domaminergic neuronal damage. College of Oriental Medicine Graduate School of KyungHee University. 2008.
  40. Seong-In Bae. Protective effects of acupuncture on MPTP-induced dopaminergic neuronal damage. College of Oriental Medicine Graduate School of KyungHee University. 2006.
  41. Kyong-ok Chu. Hyang, hyangsu, hyanggi. Seoul: Sechang Chulpansa; 1995. p. 168-171.
  42. Hyun Taek Kim, Joon Sik Choi, Eun Hue Park, Kang Hee Lee, Eun Joo Kim, Yeon Kyung Lee: Animal behavior test studying brain illness for neurologist. Seoul: Siguma Puresu; 2006. p. 112.
  43. Abe, K., Taguchi, K., Wasai, T., Ren, J. et al. : Biochemical and pathological study of endogenous 1-benzyl-1,2,3,4-tetrahydroisoquinoline-induced parkinsonism in the mouse. Brain Res, 907. p.134-.138, 2001. https://doi.org/10.1016/S0006-8993(01)02573-2
  44. The CIBA collection of medical illustrations. CIBA collection. Volume 1, PART II NERVOUS SYSTEM, Seoul: Chongdam; 2000. p. 155.
  45. Teresa Cunha-Oliveira, A. Cristina Rego, Sandra M. Cardoso, Fernanda Borges, Russell H. Swerdlow, Tice Macedo, Catarina R. : de Oliveira Brain Research, Volume 1089, Issue 1. p.44-54, 2006. https://doi.org/10.1016/j.brainres.2006.03.061
  46. Sladowski. D, Steer. S. L, Clothier. R. H, and Balls. M : An improved MTT assay. J. Immunol. Methods, 157. p.203-207, 1993. https://doi.org/10.1016/0022-1759(93)90088-O
  47. Jeong H, Koo H. Oh E, Chae H, Kim C, Cho K, et al : Nitric oxide production by high molecular weight water soluble chitosan via nuclear factor- kappa B activation. Int J Immunopharmaco,l 22. p.923-33, 2000. https://doi.org/10.1016/S0192-0561(00)00055-2
  48. Feng, L, Wang, C. Y., Jiang, H, Oho, C, Mizuno, K, Dugich-Djordjevic, M, Lu, B. : Differential effects of GDNF and BDNF on cultured ventral mesencephalic neurons. Brain Res Mol Brain Res. Mar 20;66(1-2) p.62-70. 1999. https://doi.org/10.1016/S0169-328X(99)00015-7
  49. Davis, G. C., Williams, A. C., and Markey, S. P. : Chronic Parkinsonism secondary to intravenous injection of meperidine analogues. Psychiatry Res. 1. p.249-255. 1979. https://doi.org/10.1016/0165-1781(79)90006-4
  50. Rosati, G., Granieri, E., and Pinna, L., : The risk of Parkinson's disease in Mediteranean people. Neurology, 30. p.250-255. 1980. https://doi.org/10.1212/WNL.30.3.250
  51. Ramsay RR, Dadger J, Trevor A, et al : Energy driven uptake of mpp+ by brain mitochondria mediates the neurotoxicity of MPTP. Life sci 39. o.581-588. 1986. https://doi.org/10.1016/0024-3205(86)90037-8
  52. Mizuno Y, Suzuki K, Sone N, et al : Inhibition of mitochondrial respiration by MPTP in mouse brain in vivo. Neurosci lett 91. p.349-353. 1984. https://doi.org/10.1016/0304-3940(88)90705-7
  53. Dong-Wuk Cho, Yoosik Yoon, Bong-Joo Kang, Mi-Young Lee, Seong-Gil Hong, Protective effefts of traditional oriental drugs against H2O2 induced neuronal cell death. Korea Institute of Oriental Medicine 1998;139.
  54. Seck-Hwan Kim, Joo-Yeon Lee, Y대-Jeong Kim, Hye-Ok Kang, Hang-Woo Lee, Jong-Won Choi. Effect of Selenium Yeast on MPTP(1-methyl-4-phenyl-propion-oxypipeidine)-Induced Neurotoxicity in Mice. Journal of Life Science 2006;16(2):266-273. https://doi.org/10.5352/JLS.2006.16.2.266
  55. Jong Bae Lee, Jeong Hee Park, Yong Sung Lee, Jai Kyung Koh. Effect of Reserpine Pretreatment on Neurotoxicity of N-methyl-4-pheyl-1,2,3,6-tetrahydropyridine( MPTP) in Mice. J. Hanyang Med, Coll. 1990;10(1):129-140.
  56. Wang Do. Sago Uihak Chongseo Oedae biyobang il. Sanghae: Sanghae Gojuk Chulpansa; 1991. p. 736-439.
  57. Messripour, M. and Clark, J. B. : Tyrosine hydroxylase activity in rat brain synaptosomes : Direct mesurment using high performance liquid chromatography. J. Neurochem, 38. p.1139-1143, 1982. https://doi.org/10.1111/j.1471-4159.1982.tb05360.x
  58. Sung Joon Kim, Jin Chul Yoo, Hong Sung Jeon, Jae Seong Kim, Seung Kim, Myong Hwa Sim, et al. Development and application of tyrosine hydroxylase gene and neurotrophic factor inducible material screening from medicinal herbs for prevent of neuronal cell degradation. Chosun University. 2007.
  59. Kyung Hwan Kim. Woo Joo Lee's Lecture for pharmacology. 4th ed. Seoul: Uihak Munwhasa; 1997. p. 179.
  60. Mizuno, M., Yamada, K., Olariu, A., Nawa, H., and Nabeshima, T. : Involvement of brain-derived neurotrophic factor in spatial memory fomation and maintenance in a radial arm maze tese in rats. J. Neurosci. 20, p.7116-7121. 2000.
  61. H.H. Lee, J.H. Yoon, I.G. Jeong, J.O. Kim, B.K. Kim, J.H. Seo, et al. Effects of treadmil exercise on cell proliferation and BDNF expression in the rat hippocampus after traumatic brain injury. Sports science 2008; 17(3): 363-372.
  62. Klugbauer, N., Lacinova, L., Flockerzi, V. and Hofmann, F. : Structure and functional expression of a new member of the tetrotoxin- sensitive voltage-activated sodium channel family from human neuroendocrine cells. EMBO 14, p.1084-1090. 1995.
  63. Matos, M. F., Mukherjee, K., Chen, X., Rizo, J. and Sudhof, T. C. : Evidence for SNARE zippering during $Ca^{+}$-triggered exocttosis in PC12 cells. Neuropharmacol 45. p.777-786. 2003. https://doi.org/10.1016/S0028-3908(03)00318-6
  64. Tuz, K. and Pasantes-Morales, H. : Hyposmolarity evokes norepinephrine efflux from synaptosomes by a depolarization and $Ca^{+}$-dependnet exocytotic mechanism. EUR. J. Neurosci. 22, p.1636-1642. 2005. https://doi.org/10.1111/j.1460-9568.2005.04344.x
  65. Yun-Sik Lee, Dong-Seob Kim. Effect of FS11052, an Inhibitor of Exocytosis, on Neurite Extension in Rat Hippocampal Neurons and PC12 Cells. Journal of Life Science 2006; 16(2): 315-322. https://doi.org/10.5352/JLS.2006.16.2.315
  66. O'Conner, V. M., O. Shmotienko, E. Grishin and H. Bet. : On the structure of the synapto-'secretosome'-Evidence for a rerexin/synaptotagmin/ syntaxin/$Ca^{+}$ channel complex. FEBS 326. p.255-26. 1993. https://doi.org/10.1016/0014-5793(93)81802-7

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