DOI QR코드

DOI QR Code

Antioxidative Activity of Prunus sargentii Outer Bark Extractives

  • Park, Se-Yeong (Dept. of Forest Biometerials Engineering, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Bae, Young-Soo (Dept. of Forest Biometerials Engineering, College of Forest and Environmental Sciences, Kangwon National University)
  • Received : 2012.03.14
  • Accepted : 2012.03.22
  • Published : 2012.03.25

Abstract

The outer bark of Prunus sargentii was collected, air-dried and extracted with 70% aqueous acetone. Then it was successively partitioned with n-hexane, dichloromethane ($CH_2Cl_2$), ethyl acetate (EtOAc) and $H_2O$. From the EtOAc soluble fraction, four compounds were isolated by the repeated Sephadex LH-20 column chromatography. The isolated compounds were determined as (+)-catechin (1), (-)-epicatechin (2), taxifolin (3), and neosakuranin (4) by the spectroscopic analysis including $^1H$, $^{13}C$-NMR, and 2D-NMR spectrometers. The antioxidative activities on the isolated compounds and the separated fractions were evaluated by DPPH radical scavenging assay. The crude, EtOAc, and $H_2O$ soluble fractions indicated good antioxidative potential compared to the $CH_2Cl_2$ and n-hexane soluble fractions.

Keywords

References

  1. Lee, H. J., S. S. Lee, D. H. Choi, and A. Kato. 2001. Studied on Biological Activity of Wood Extractives -Flavanoids in heartwood of Prunus sargentii.-. Mokchae Konghak 29(2): 133-139
  2. Lee, S. S., H. J. Lee, and D. H. Choi. 2001. Studied on Biological Activity of Wood ExtractivesVIII: Antimicrobial and Antioxidation Activities of Extractives from the Heartwood of Prunus sargentii.-Mokchae Konghak 29(2): 140-145
  3. Biois, M. S. 1985. Antioxidant determinations by the use of a stable free radical. Natural 26: 1199-1200.
  4. Gutfinger, T. 1981. Polyphenols in olive oils. Journal of the American Oil Chemists' Society 58: 966-968. https://doi.org/10.1007/BF02659771
  5. Harborne, J. B. and T. J. Marby. 1982. The flavonoids: advances in research, Chapman and Hall LTd., London, UK, 6: 35-37.
  6. Agrawal, P. K. 1989. Carbon-13 NMR of flavanoids, Elsevier, New York.
  7. Ishimaru, K., T. Omoto, I. Asai, K. Ezaki, and K, Shimomura. 1995. Taxifolin 3-arabinoside from Fragariax ananassa. Phytochemistry 40(1): 345 -347. https://doi.org/10.1016/0031-9422(95)00204-K
  8. Jenkins, T., J. Bhattacharyya, G. Majetich, Q. Teng, A. M. Fatima, and R. Almeida. 1999. Flavonoids from the root-bark of Dioclea grandiflra. Phytochemistry 52(4): 723-730. https://doi.org/10.1016/S0031-9422(99)00314-3
  9. Bhatt, L. R, M. S. Bae, B. M. Kim, G. S. Oh, and K. Y. Chai. 2009. A Chalcone Glycoside from the Fruits of Sorbus commixta Hedl. Molecules 14: 5323-5327. https://doi.org/10.3390/molecules14125323
  10. Jayaprakasam, B., D. Gunasekar, K. V. Rao, A. Blond, and B. Bodo. Androechin. 2001. A new chalconeglucoside from Andrographis Echioides. J. Asian Nat. Prod. 3; 43-48. https://doi.org/10.1080/10286020108042837
  11. Zhang, X. F., T. M. Hung, P. T. Phuong, T. M. Ngoc, B. S. Min, K. S. Song, Y. H. Seong, and K. H. Bae. 2006. Anti-inflammatory activity of flavonoids from Populus davidiana. Arch. Pharm. Res. 29: 1102-1108. https://doi.org/10.1007/BF02969299
  12. Ogawa, Y., H. Oku, E. Iwaoka, M. Iinuma, and K. Ishiguro. 2006. Allergy-preventive phenolic glycosides from Populus sieboldii. J. Nat. Prod. 69: 1215-1217. https://doi.org/10.1021/np0505216
  13. Djeridane, A., M. Yousfi, B. Nadjemi, D. Boutassouna, P. Stocker, and N. Vidal. 2006. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 97(4): 654-660 https://doi.org/10.1016/j.foodchem.2005.04.028
  14. Si, C. L. 2006. Studies on the Chemical Constituents and Antioxidative Activities of Paulownia coreana Uyeki. Ph. D. Thesis.