Clinics in Shoulder and Elbow

대한견·주관절의학회 (Korean Shoulder and Elbow Society)
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줄기세포의 분화능의 기원에 따른 비교 - 견봉하 점액낭, 골수, 탯줄 혈액 -

Differential Potential of Stem Cells Following Their Origin - Subacromial Bursa, Bone Marrow, Umbilical Cord Blood -

  • 심성우 (조선대학교 의과대학 정형외과학교실) ;
  • 문영래 (조선대학교 의과대학 정형외과학교실) ;
  • 강정훈 (조선대학교 의과대학 정형외과학교실)
  • Sim, Sung Woo (Departments of Orthopedics, Chosun University Hospital) ;
  • Moon, Young Lae (Departments of Orthopedics, Chosun University Hospital) ;
  • Kang, Jung Hun (Departments of Orthopedics, Chosun University Hospital)
  • 투고 : 2012.03.15
  • 심사 : 2012.08.22
  • 발행 : 2012.12.31
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초록

목적: 세가지 기원의 줄기 세포 분화능과 면역표현형을 평가하고자 하였다. 대상 및 방법: 견봉하 점액낭과 골수, 탯줄 혈액 세 개의 군에서 세포를 채취하였다. 견봉하 점액낭과 골수는 견관절 수술 환자군에게 임상적 동의 하에 수술중 채취하였다. 각각의 채취된 세포 및 탯줄 혈액에 대하여 계대 배양을 시행하여 신경 분화군, 지방 분화군, 골 분화군을 평가하였으며 세포 표면 항체를 밝히기 위해 유동세포분석법을 이용하였다. 결과: 견봉하 점액낭 유래 세포에서는 신경분화와 지방 분화는 8예 모두 (100%)에서, 골분화는 8례 중 5예 (62.5%)에서 성공할 수 있었으며 골수 유래 세포의 경우 신경 및 지방 분화 유도한 6례 및 5예 모두 (100%) 분화에 성공하였으나 골분화 유도는 5예 중 4예 (80%)에서 얻을 수 있었다. 반면 탯줄 유래 세포 분화 연구의 경우 신경 분화 유도 67례 중 65예 (97%)에서 지방 분화 연구 54예 중 29예 (53.7%)에서 골 분화 연구 57예 중 39예 (68.4%)에서 성공할 수 있었다. 결론: 탯줄 유래 줄기세포의 분화능과 비교하였을 때 견봉하 점액낭 및 골수 유래 줄기세포의 분화능이 우수함을 알 수 있으며 이는 향후 세포 치료에 있어서 안정성 있는 치료 제공자가 될 수 있을 것으로 보이며 향후 생체 실험 연구의 참고 자료로서도 가치가 있을 것으로 보인다.

Purpose: To evaluate the differentiation potential of stem cells and their immunophenotype from 3 different sources. Methods: Our study involved three stem cell sources-subacromial bursal tissue, bone marrow, and umbilical cord blood. We obtained the subacromial bursal tissue and bone marrow from the patients undergoing shoulder surgery. After collecting the sample, we applied specific induction media for neurogenic, adipogenic and osteogenic differentiation. Also, flow-cytometry analysis was done to reveal the cell surface antigens. Results: We obtained 100% (8 cases) neural and adipogenic differentiation, but 62.5% (5 of 8 cases) osseous differentiation among the subacromial bursal tissue group. Bone marrow derived cells showed 100% neural (6 cases) and adipogenic (5 cases) differentiation, but 80% (4 of 5 cases) osseous differentiation. Umbilical cord blood derived cells revealed 97% (65 of 67 cases) neural, 53.7% (29 of 54 cases) adipogenic and 68.4% (39 of 57 cases) osseous differentiation. Immunophenotype analysis revealed that surface markers of bone marrow, subacromial bursal cell and umbilical cord blood derived mesenchymal stem cells are different from each other. Conclusions: Mesenchymal stem cells are potential agents in regenerative medicine and are characterized by expression of surface markers and by their differentiation potential. Our study with stem cells from subacromial bursal tissue, bone marrow and umbilical cord discovered that each stem cell has unique differentiation potential and function based on its origin. Various stem cells show multi-lineage differentiations in vitro which can be correlated to in vivo conditions.

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참고문헌

  1. Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol. 2004;36:568-84. https://doi.org/10.1016/j.biocel.2003.11.001
  2. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143-7. https://doi.org/10.1126/science.284.5411.143
  3. Yang SE, Ha CW, Jung M, et al. Mesenchymal stem/progenitor cells developed in cultures from UC blood. Cytotherapy. 2004;6:476-86. https://doi.org/10.1080/14653240410005041
  4. Niibe K, Kawamura Y, Araki D, et al. Purified mesenchymal stem cells are an efficient source for iPS cell induction. PLoS One. 2011;6:e17610. https://doi.org/10.1371/journal.pone.0017610
  5. Peyrard T, Bardiaux L, Krause C, et al. Banking of pluripotent adult stem cells as an unlimited source for red blood cell production: potential applications for alloimmunized patients and rare blood challenges. Transfus Med Rev. 2011;25:206-16. https://doi.org/10.1016/j.tmrv.2011.01.002
  6. Satomura K, Krebsbach P, Bianco P, Gehron Robey P. Osteogenic imprinting upstream of marrow stromal cell differentiation. J Cell Biochem. 2000;78:391-403. https://doi.org/10.1002/1097-4644(20000901)78:3<391::AID-JCB5>3.0.CO;2-E
  7. Shafiee A, Kabiri M, Ahmadbeigi N, et al. Nasal Septum-Derived Multipotent Progenitors: A Potent Source for Stem Cell-Based Regenerative Medicine. Stem Cells Dev. 2011;20:2077-91. https://doi.org/10.1089/scd.2010.0420
  8. Ratajczak MZ, Zuba-Surma EK, Machalinski B, Kucia M. Bone-marrow-derived stem cells--our key to longevity?. J Appl Genet. 2007;48:307-19. https://doi.org/10.1007/BF03195227
  9. Reyes M, Verfaillie CM. Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells. Ann N Y Acad Sci. 2001;938:231-3. discussion 3-5.
  10. Yoon SH, Shim YS, Park YH, et al. Complete spinal cord injury treatment using autologous bone marrow cell transplantation and bone marrow stimulation with granulocyte macrophage-colony stimulating factor: Phase I/II clinical trial. Stem Cells. 2007;25:2066-73. https://doi.org/10.1634/stemcells.2006-0807
  11. Kern S, Eichler H, Stoeve J, Kluter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006;24:1294-301. https://doi.org/10.1634/stemcells.2005-0342
  12. Uhthoff HK, Trudel G, Himori K. Relevance of pathology and basic research to the surgeon treating rotator cuff disease. J Orthop Sci. 2003;8:449-56. https://doi.org/10.1007/s10776-002-0624-5
  13. Moon YL, Song CH, Noh KH, Lee SJ, Lee HJ, Kim SH. Isolation and Phenotypic Characterization of Multipotent Mesenchymal-like Stem Cells from Human Subacromial Bursa. J Tissue Eng Regen Med. 2009;5:527-32.
  14. Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8:315-7. https://doi.org/10.1080/14653240600855905
  15. Mareschi K, Biasin E, Piacibello W, Aglietta M, Madon E, Fagioli F. Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood. Haematologica. 2001;86:1099-100.

피인용 문헌

  1. Arthroscopic Repair of Partial-Thickness and Small Full-Thickness Rotator Cuff Tears vol.43, pp.3, 2015, https://doi.org/10.1177/0363546514561004
  2. Pain and Power Doppler Ultrasonographic Evaluation according to Bursal Preservation after Arthroscopic Rotator Cuff Repair: Comparison between Complete and Minimal Bursectomy in Early 3 Months vol.53, pp.3, 2018, https://doi.org/10.4055/jkoa.2018.53.3.218