한국가금학회지 (Korean Journal of Poultry Science)

  • 제39권4호
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  • Pages.273-278
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  • 2012
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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포도상구균에서 분비하는 장내독소 B(SEB)에 대한 재조합 단백질을 이용한 면역특이적 난황항체 생산

Production of Immunospecific Egg Yolk Antibody with Recombinant Staphylococcal Enterotoxin B (SEB) Protein

  • 이성 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 이상래 (한국생명공학연구원 국가영장류센터) ;
  • 정경민 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 김정우 (단국대학교 생명자원과학대학 동물자원학과)
  • Lee, Seong (Department of Animal Resources and Science, Dankook University) ;
  • Lee, Sang-Rae (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung, Kyung Min (Department of Animal Resources and Science, Dankook University) ;
  • Kim, Jung Woo (Department of Animal Resources and Science, Dankook University)
  • 투고 : 2012.10.15
  • 심사 : 2012.12.04
  • 발행 : 2012.12.31
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초록

본 연구는 식중독은 물론, 아토피성 피부염의 원인물질로 알려져 있는 포도상구균 장내독소 B(Staphylococcal enterotoxin B; SEB)에 대한 특이 난황항체를 개발하고자 실험을 실시하였다. 우선, SEB 유전자를 클로닝한 다음, 대장균 발현시스템을 이용하여 약 30 kDa 정도의 재조합 SEB 단백질을 생산하였다. 재조합 SEB 단백질을 산란계에 2주 간격으로 3회 면역접종을 실시하고, 혈청 및 난황 내 항체가를 측정한 결과, 면역 후 4주경에 항체가가 최고치에 달하였으며, 산란계로부터 획득한 난황항체를 이용한 Western blot 결과, 재조합 SEB 단백질은 물론, 상용화 SEB 단백질과도 특이적으로 반응한다는 것을 규명하였다. 결론적으로, 식중독과 아토피성 피부염 등의 원인물질로 알려진 SEB에 특이적인 난황항체를 생산에 성공하였으며, 이러한 특이적인 난황항체는 식중독 및 아토피성 피부염의 예방 및 치료에 활용 가능할 것으로 사료된다.

Staphylococcal enterotoxin B (SEB), which is a bacterial superantigen produced by Staphylococcus aureus, is associated with serious diseases, including food poisoning and atopic dermatitis. This study was performed to produce about 30 kDa of recombinant SEB protein and to immunize in chickens to acquire the specific egg yolk antibody (IgY) against the recombinant SEB. Chickens were immunized with the recombinant SEB intramuscularly in the breast muscle by injection 3 times at intervals of two weeks. Serum- and egg yolk-antibody titers of hens against SEB were highest at 4 weeks after first immunization. In western blot, anti-recombinant SEB IgY was reacted immunospecifically against the recombinant SEB and commercialized SEB. These results suggested that the recombinant SEB antigen could be used as an immunogen to elicit antibody (IgY) against SEB and the anti-recombinant SEB IgY could neutralized staphylococcal enterotoxin B effectively.

키워드

참고문헌

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