The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of Tyrosinase Inhibitor from Saccharomyces cerevisiae

Saccharomyces cerevisiae 로부터 Tyrosinase Inhibitor 의 생산

  • Jang, In-Taek (Department of Biomedicinal Science and Biotechnology, Pachai University) ;
  • Kim, Young-Hun (Department of Biomedicinal Science and Biotechnology, Pachai University) ;
  • Kang, Min-Gu (Department of Biomedicinal Science and Biotechnology, Pachai University) ;
  • Yi, Sung-Hun (Korea Food Research Institute) ;
  • Lim, Sung-Il (Korea Food Research Institute) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Pachai University)
  • 장인택 (배재대학교 바이오의생명공학과) ;
  • 김영헌 (배재대학교 바이오의생명공학과) ;
  • 강민구 (배재대학교 바이오의생명공학과) ;
  • 이성훈 (한국식품연구원) ;
  • 임성일 (한국식품연구원) ;
  • 이종수 (배재대학교 바이오의생명공학과)
  • Received : 2012.03.09
  • Accepted : 2012.03.21
  • Published : 2012.04.01
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Abstract

Physiological functionalities of culture concentrates from various fungi were investigated. The culture concentrates from Saccharomyces cerevisiae Y277-3 showed the highest tyrosinase inhibitory activity of 42.7%. Among mold physiological functionalities, the culture concentrates from Aspergillus orygae CN20-3-1-4 showed the highest antioxidant activity of 15.8%. The other functionalities of fungi were very low or not detected. The intracellular tyrosinase inhibitor from Saccharomyces cerevisiae Y277-3, which showed the highest physiological functionalities was maximally produced when the strain was cultured in PD broth at $30^{\circ}C$ for 24 h.

한국식품연구원으로부터 분양받은 효모 49종과 곰팡이 34종등 총 83종의 진균류들의 PD broth 배양 농축물들의 생리 기능성을 측정 하였다. 효모 배양 농축물중에서는 Saccharomyces cerevisiae Y277-3 과 Saccharomyces cerevisiae Y157-1 의 PD broth 배양 농축물들의 tyrosinase 저해활성이 각각 42.7%와 41.2%로 생리기능성중 제일 높은 활성을 보였다. 또한 곰팡이 배양 농축물에서는 Aspergillus oryzae CN20-3-1-4 배양 농축물의 항산화 활성이 15.8%로 가장 높았으나 대체로 낮은 생리기능성을 보였다. 시험 진균류들의 생리기능성중 가장 높았던 Saccharomyces cerevisiae Y277-3 의 세포내 tyrosinase 저해물질은 이 효모를 PD broth 에 접종하여 $30^{\circ}C$에서 24시간 배양 하였을때 가장 많이 생산 되었다.

Keywords

References

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