한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제29권6호
  • /
  • Pages.460-463
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  • 2012
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

성장 돼지 적혈구에서의 항산화 효소 활성도에 대한 propofol 과 isoflurane 병용 투여의 영향

An Influence of a Combined Administration of Propofol and Isoflurane on Antioxidative Enzyme Activities in Growing Swine Erythrocytes

  • 이재연 (충남대학교 수의과대학.동물의과학연구소) ;
  • 김명철 (충남대학교 수의과대학.동물의과학연구소)
  • Lee, Jae Yeon (College of Veterinary Medicine.Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung Cheol (College of Veterinary Medicine.Research Institute of Veterinary Medicine, Chungnam National University)
  • 심사 : 2012.12.04
  • 발행 : 2012.12.31
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초록

본 연구에서는 돼지에서 수술 시 propofol 및 isoflurane 투여가 생체내 항산화효소 활성도에 미치는 영향을 연구하였다. 실험동물은 수술에 사용되는 마취 종류에 따라 isoflurane 그룹 (group 1; 100% 산소 및 2-2.5% isoflurane 투여)과 isoflurane-propofol 그룹 (group 2; 8 mg/kg/h propofol 정맥 투여, 100% 산소 및 0.5-1% isoflurane 투여)으로 나누었다. 그룹 1에서는 마취 전과 비교 시 수술 후 생체내 Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) 활성도가 유의적으로 낮아졌으나 그룹 2에서는 마취 전 수준을 유지하였다. 또한 모든 효소 수치에서 군간 비교 시 유의성 있는 변화가 관찰되었다. 본 연구 결과를 통해 propofol의 투여가 돼지에서 마취 및 수술 중 항산화 능력을 유지 할 수 있음을 확인 할 수 있었다.

The present study was aimed to evaluate and compare the oxidative stress status of isoflurane and propofol in pigs undergoing surgery with measuring the activities of antioxidant enzymes. The pigs were divided into 2 groups according to the type of anesthesia used for the surgical procedure. In the isoflurane group (group 1), anesthesia was induced and maintained with 2-2.5% isoflurane under 100% oxygen. The propofol group (group 2) received 8 mg/kg/h of IV propofol with 0.5-1% isoflurane under 100% oxygen. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities of isoflurane group were significantly lower at the end of surgery than at induction of anesthesia, while that of the propofol group maintained their baseline values. There were significant differences in all enzymes activities between groups at the end of surgery. These results indicate that propofol is capable of preserving the antioxidant capacity in pigs anesthetized with the combination of isoflurane and propofol infusion.

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참고문헌

  1. Allaouchiche B, Debon R, Goudable J, Chassard D, Duflo F. Oxidative stress status during exposure to propofol, sevoflurane and desflurane. Anesth Analg 2001; 93: 981-985. https://doi.org/10.1097/00000539-200110000-00036
  2. Aarts L, van der Hee R, Dekker I, de Jong J, Langemeijer H, Bast A. The widely used anesthetic agent propofol can replace alpha-tocopherol as an antioxidant. FEBS Letters 1995; 2: 83-85.
  3. De La Cruz JP, Sedeño G, Carmona JA, Sánchez de la Cuesta F. The in vitro effects of propofol on tissular oxidative stress in the rat. Anesth Analg 1998; 87: 1141-1146.
  4. De La Cruz JP, Zanca A, Carmona JA, de la Cuesta FS. The effect of propofol on oxidative stress in platelets from surgical patients. Anesth Analg 1999; 89: 1050-1055.
  5. Halliwell B. Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med 1991; 30: 14S-22S.
  6. Kato R, Foex P. Myocardial protection by anesthetic agents against ischemia-reperfusion injury: an update for anesthesiologists. Can J Anesth 2002; 49: 777-791. https://doi.org/10.1007/BF03017409
  7. Kobayashi K, Yoshino F, Takahashi SS, Todoki K, Maehata Y, Komatsu T, Yoshida K, Lee MC. Direct assessments of the antioxidant effects of propofol medium chain triglyceride/ long chain triglyceride on the brain of stroke-prone spontaneously hypertensive rats using electron spin resonance spectroscopy. Anesthesiology 2008; 109: 426-435. https://doi.org/10.1097/ALN.0b013e318182a903
  8. Koksal H, Kurban S. Total oxidant status, total antioxidant status, and paraoxonase and arylesterase activities during laparoscopic cholecystectomy. Clinics 2010; 65: 285-290. https://doi.org/10.1590/S1807-59322010000300008
  9. Michels C, Raes M, Toussaint O, Remacle J. Importance of Se-glutathione peroxidase, catalase, and Cu/Zn SOD for cell survival against oxidative stress. Free Radic Biol Med 1994;17: 235-248. https://doi.org/10.1016/0891-5849(94)90079-5
  10. Plummer JL, Beckwith AL, Bastin FN, Adams JF, Cousins MJ, Hall P. Free radical formation in vivo and hepatotoxicity due to anesthesia with halothane. Anesthesiology 1982; 57:160-166. https://doi.org/10.1097/00000542-198209000-00003