Extracellular Prostaglandin $E_2$ Upregulation Effect of the Methanol Extract of Artemisia argyi

  • Lee, Kyoung In (Biotechnology Industrialization Center, Dongshin University) ;
  • Moon, Young Sook (Research Center for Resistant Cells, Chosun University) ;
  • Pyo, Byoung Sik (Department of Oriental Medicine Materials, Dongshin University) ;
  • Choi, Chul Hee (Research Center for Resistant Cells, Chosun University)
  • Received : 2012.08.29
  • Accepted : 2012.09.21
  • Published : 2012.12.31

Abstract

Since 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is the key metabolic enzyme of prostaglandin $E_2$ ($PGE_2$), inhibition of 15-PGDH is supposed to facilitate various physiological functions by increasing $PGE_2$. Methanol extract of Artemisia argyi (AAME) inhibited 15-PGDH ($IC_{50}$: $13.13{\mu}g/mL$) with relatively low cytotoxicity ($IC_{50}$: $415.00{\mu}g/mL$) and elevated extracellular $PGE_2$ levels in HaCaT cells. Real-time PCR analysis showed that AAME decreased significantly mRNA expression of PG transporter (PGT) in HaCaT cells. These results indicate that AAME could be applicable to functional materials as a 15-PGDH inhibitor and PGT expression inhibitor for the upregulation of extracellular $PGE_2$ level.

Keywords

References

  1. Anggard, E. and Samuelsson, B., Smooth muscle stimulating lipids in sheep Iris. The identification of prostaglandin F 2a. Prostaglandins and related factors 21. Biochem. Pharmacol. 13, 281-283 (1964). https://doi.org/10.1016/0006-2952(64)90146-7
  2. Araki, H., Komoike, Y., Matsumoto, M., Tanaka, A., and Takeuchi, K., Healing of duodenal ulcers is not impaired by indomethacin or rofecoxib, the selective COX-2 inhibitor, in rats. Digestion. 66, 145- 153 (2002). https://doi.org/10.1159/000066759
  3. Balsinde, J., Balboa, M.A., Insel, P.A., and Dennis, E.A., Regulation and inhibition of phospholipase A2. Annu. Rev. Pharmacol. Toxicol. 39, 175-89 (1999). https://doi.org/10.1146/annurev.pharmtox.39.1.175
  4. Chan, M.W., Wong, C.Y., Cheng, A.S., Chan, V.Y., Chan, K.K., To, K.F., Chan, F.K., Sung, J.J, and Leung, W.K., Targeted inhibition pf COX-2 expression by RNA interference suppresses tumor growth and potentiates chemosensitivity to cisplatin in human gastric cancer cells. Oncol. Rep. 18, 1557-1562 (2007).
  5. Choi, S.R., You, D.H., Kim, J.Y., Park, C.B., Ryu, J., Kim, D.H., and Eun, J.S., Antioxidant and antimicrobial activities of Artemisia capillaris Thunberg. Korean J. Medicinal Crop Sci. 16, 112-117 (2008).
  6. Ding, Y., Liang, C., Choi, E.M., Ra, J.C., and Kim, Y.H., Chemical constituents from Artemisia iwayomogi increase the function of osteoblastic MC3T3-E1 cells. Natural Product Science. 15, 192-197 (2009).
  7. Fujimoto, Y., Sakuma, S., Komatsu, S., Sato, D., Nishida, H., Xiao, Y.Q., Bada, K., and Fujita, T., Inhibition of 15-hydroxyprostaglandin dehydrogenase activity in rabbit gastric antral mucosa by panaxynol isolated from oriental medicines. J. Pharm. Pharmacol. 50, 1075- 1078 (1998). https://doi.org/10.1111/j.2042-7158.1998.tb06925.x
  8. Harizi, H., Grosset, C., and Gualde, N., Prostaglandin E2 modulates dendritic cell function via EP2 and EP4 receptor subtypes. J. Leukoc. Biol. 73, 756-763 (2003). https://doi.org/10.1189/jlb.1002483
  9. Iijima, Y., Kawakita, N., and Yamazaki, M., Inhibition of 15- hydroxyprostaglandin dehydroxygenase by antiallegic agents. Biochem. Biophys. Res. Commun. 93, 912-918 (1980). https://doi.org/10.1016/0006-291X(80)91162-6
  10. Kolodsick, J.E., Peters-Golden, M., Lario,s J., Toews, G.B., Thannickal, V.J., and Moore, B.B., Prostaglandin E2 inhibits fibroblast to myofibroblast transition via E. prostanoid receptor 2 signaling and cyclic adenosine monophosphate elevation. Am. J. Respir. Cell Mol. Biol. 29, 537-544 (2003) https://doi.org/10.1165/rcmb.2002-0243OC
  11. Konger, R.L., Malaviya, R., and Pentland, A.P., Growth regulation of primary human keratinocytes by prostaglandin E receptor EP2 and EP3 subtypes. Biochim. Biophys. Acta. 1401, 221-234 (1998). https://doi.org/10.1016/S0167-4889(97)00114-6
  12. Lee, M.K., Choi, G.P., Ryu, L.H., Lee, G.Y., Yu, C.Y., and Lee, H.Y., Enhanced immune activity and cytotoxicity of Artemisia capillaris Thunb. extracts against human cell lines. Korean J. Medicinal Crop Sci. 12, 36-42 (2004).
  13. Lee, S.M., Kim, M.G., Lee, S.Y., and Kang, T.H., Effects of Artemisia pronceps extract on bone metabolism. J. Korean Soc. Food Sci. Nutr. 39, 363-368 (2010). https://doi.org/10.3746/jkfn.2010.39.3.363
  14. Li, D., Han, X.H., Hong, S.S., Lee, C., Lee, M.S., Lee, D.H., Lee, M.K., and Hwang, B.Y., Inhibitors of nitric oxide production from Artemisia princeps. Natural Product Science. 16, 143-147, (2010).
  15. Parekh, A., Sandulache, V.C., Singh, T., Cetin, S., Sacks, M.S., Dohr, J.E., and Hebda, P.A., Prostaglandin E2 differentially regulates contraction and structural reorganization of anchored collagen gels by human adult and fetal dermal fibroblasts. Wound Repair Regen. 17, 88-98 (2009). https://doi.org/10.1111/j.1524-475X.2008.00445.x
  16. Park, W.S., Study on biological effect of water extract from Artemisia argi folium on mouse macropharge Raw 264.7 cells. Korean J.Oriental Physiology & Pathology. 22, 815-820 (2008).
  17. Park, W.S., Effect of water extract from Artemisia argi folium on mouse macropharge stimulated by LPS. Korean J. Herbology. 24, 151-157 (2009).
  18. Pei, S.J., Ethnobotanical approaches of traditional medicine srudies: Some experiences from Asia. Pharm. Biol. 39, 74-79 (2001).
  19. Sandulache, V.C., Parekh, A., Li-Korotky, H., Dohar, J.E., and Hebda, P.A., Prostaglandin E2 inhibition of keloid fibroblast migration, contraction, and transforming growth factor (TGF)-1-induced collagen synthesis. Wound Repair Regen. 15, 122-133 (2007). https://doi.org/10.1111/j.1524-475X.2006.00193.x
  20. Schuster, V.L., Prostaglandin transport. Prostaglandins Other Lipid Mediat. 68-69, 633-647 (2002).
  21. Schuster, V.L., Molecular mechanisms of prostaglandin transport. Annu. Rev. Physiol. 60, 221-242 (1998). https://doi.org/10.1146/annurev.physiol.60.1.221
  22. Takehara, H., Iwamoto, J., Mizokami, Y., Takahashi, K., Ootubo, T., Miura, S., Narasaka, T., Takeyama, H., Omata, T., Shimokobe, K. Ito, M., and Matsuoka, T., Involvement of cyclooxygenase-2-- prostaglandin E2 pathway in interleukin-8 production in gastric cancer cells. Dig. Dis. Sci. 51, 2188-2197 (2006). https://doi.org/10.1007/s10620-006-9436-2
  23. Wallace, J.L., Prostaglandins, NSAIDs, and gastric mucosal protection: why doesn't the stomach digest itself? Physiol. Rev. 88, 1547-1565 (2008). https://doi.org/10.1152/physrev.00004.2008
  24. Wilgus, T.A., Bergdall, V.K., Tober, K.L., Hill, K.J., Mitra, S., Flavahan, N.A., and Oberyszyn, T.M., The impact of cyclooxygenase-2 mediated inflammation on scarless fetal wound healing. Am. J. Pathol. 165, 753-761 (2004). https://doi.org/10.1016/S0002-9440(10)63338-X