Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Preparation of CdSe QDs-carbohydrate Conjugation and its Application for HepG2 Cells Labeling

  • Jiang, Mingxing (School of Life Science, Anhui University) ;
  • Chen, Yan (School of Life Science, Anhui University) ;
  • Kai, Guiqing (School of Life Science, Anhui University) ;
  • Wang, Ruijun (School of Life Science, Anhui University) ;
  • Cui, Huali (School of Life Science, Anhui University) ;
  • Hu, Meili (School of Life Science, Anhui University)
  • Received : 2011.11.30
  • Accepted : 2011.12.14
  • Published : 2012.02.20
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Abstract

In present study, CdSe quantum dots (QDs) were prepared with a novel but simple, effective and exercisable method. Nine different types of carbohydrate molecules were used to modify CdSe QDs. D-mannose (Man)-coated quantum dots were prepared for labeling human hepatoma (HepG2) cells, because of the high expression of mannose receptor (MR) on HepG2 cells. The uptake characteristics of CdSe QDs-Man were investigated in HepG2 cells. The absorption rate result of MTT assay in 48 h suggested the extremely low cytotoxicity of CdSe QDs-Man. The presence of quantum dots was confirmed with fluorescence microscopy. These results were encouraging regarding the application of QDs molecules for early detection of HepG2 cells.

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