Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Hepatoprotective Effects of Various Enzyme Hydrolysates from Oysters on Tacrine-Induced Toxicity in Human Hepatoma Cells

타크린으로 유발한 간세포 독성에 대한 효소별 굴 가수분해물의 보호 효과

  • Park, Hye-Jin (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Do, Hyung-Joo (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Kim, Ok-Ju (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Kim, Andre (Department of Bioscience and Biotechnology, Graduate school, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Graduate school, Silla University)
  • 박혜진 (신라대학교 대학원 생명공학과) ;
  • 도형주 (신라대학교 대학원 생명공학과) ;
  • 김옥주 (신라대학교 대학원 생명공학과) ;
  • 김안드레 (신라대학교 제약공학과) ;
  • 하종명 (신라대학교 대학원 생명공학과)
  • Received : 2011.12.02
  • Accepted : 2012.01.19
  • Published : 2012.01.30
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Abstract

This study investigated the potential hepatoprotective benefits of Crassostrea gigas oyster hydrolysates. Oysters are known to have many biofunctional properties. In particular, oyster enzymatic hydrolysates produce substances with beneficial functions. The potential hepatoprotective effects of C. gigas hydrolysates against damage induced by tacrine were evaluated in vitro in HepG2 cells. Peptides were generated from C. gigas by enzymatic hydrolysis with Neutrase, Flavourzyme, or Protamex enzyme preparations. Tacrine treatment induced considerable cell damage in HepG2 cells, as shown by significant leakage of glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH). Cells treated with C. gigas hydrolysates showed an increased resistance to oxidative challenge compared to control cells, as revealed by higher cell survival against tacrine-induced hepatotoxicity. In addition, treatment with C. gigas hydrolysates reduced the leakage of GOT and LDH. These findings indicate that enzyme hydrolysates derived from C. gigas may be of benefit for developing hepatoprotective foods and drugs.

본 연구는 굴 가수분해물의 간 보호 효과를 확인하는 것을 목표로 하였다. 굴은 많은 기능적 요소를 가지고 있다고 알려져 있으며 특히, 효소에 의해 생산된 가수분해물은 우수한 기능적 물질을 포함한다. 타크린으로 유발한 간세포 독성에 대한 효소별 굴 가수분해물의 보호 효과를 확인하기 위하여 HepG2세포를 이용하여 in vitro상에서 확인하였다. 사용한 샘플은 Neutrase, Flavourzyme, Protamex을 이용하여 효소 가수분해한 것이다. 타크린으로 손상을 유발한 간세포에서는 GOT와 LDH가 증가하게 된다. 굴 효소 가수분해물을 처리한 실험군에서는 아무런 처리를 하지 않은 실험군에 비하여 높은 세포 생존율을 확인할 수 있었다. 또한 GOT와 LDH 역시 감소하는 것을 알 수 있었다. 본 연구를 통하여 타크린 독성에 대한 신약, 식품의 기초 자료를 제공할 수 있을 것으로 기대된다.

Keywords

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