Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

The Influence of Dietary Calcium and Phosphorus Imbalance on Intestinal NaPi-IIb and Calbindin mRNA Expression and Tibia Parameters of Broilers

  • Li, Jianhui (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Yuan, Jianmin (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Guo, Yuming (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Sun, Qiujuan (State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University) ;
  • Hu, Xiaofei (Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Science)
  • Received : 2011.08.02
  • Accepted : 2011.11.02
  • Published : 2012.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

A $2{\times}2$ factorial experiment was conducted to study the effect of dietary calcium and non-phytate phosphorus (nPP) imbalance on calbindin and NaPi-IIb mRNA levels in the small intestine and tibia parameters of broiler chicks. One hundred and forty four 1-d-old Arbor Acres male broiler chicks were divided into four treatments consisted of six replicates with six chicks each. The two dietary calcium levels were 1.10% and 0.60%, and two dietary nPP levels were 0.50% and 0.27%. Results showed that a high Ca/nPP ratio diet (4.07:1) significantly depressed feed intake and weight gain of broilers (p<0.05), but a lower Ca:nPP ratio (1.2:1) had no influence (p>0.05). Low-Ca with low-P diet resulted in low tibia minerals and tibia breaking strength of broilers, and all the tibia parameters were further decreased when the dietary ratio of Ca to P was relative higher. Low dietary Ca or P up-regulated the calbindin and NaPi-IIb mRNA expression levels. Low Ca with normal P diet up-regulated duodenal calbindin mRNA expression level to the greatest extent. Low P with a normal Ca diet significantly enhanced NaPi-IIb mRNA expression level to the highest extent. These results suggest that the calbindin and NaPi-IIb mRNA expression were enhanced by the imbalance between dietary Ca and nPP, and their expression were not only influenced by Ca or nPP level, but also the ratio of Ca:nPP.

Keywords

References

  1. Al-Masri, M. R. 1995. Absorption and endogenous excretion of phosphorus in growing broiler chicks, as influenced by calcium and phosphorus ratios in feed. Br. J. Nutr. 74:407-415. https://doi.org/10.1079/BJN19950144
  2. Ayasan, T. and F. Okan. 1999. The effects of dietary calcium and phosphorus levels on egg production traits and egg shell quality in Japanase quail. Hayvansal Uretim. 39-40:98-104.
  3. Berner Y. N. and S. Moshe. 1988. Consequences of phosphate imbalance. Annu. Rev. Nutr. 8:121-148. https://doi.org/10.1146/annurev.nu.08.070188.001005
  4. Blahos, J., A. D. Care and B. S. Sommerville. 1987. Effect of low calcium and low phosphorus diets on duodenal and ileal absorption of phosphate in chick. Endocrinol. Exp. 21:59-64.
  5. Cheng, J. K. and G. X. Fan. 1993. Effects of high dietary calcium and low dietary phosphorus on acid-base balance in chickens. J. Beijing. Agric. College. 8:85-88.
  6. Danisi, G. and H. Murer. 1991. Inorganic phosphate absorption in small intestine. In Handbook of Physiology, section 6, vol IV, chapter 12 (Ed. S. G. Schutt, M. Field and R. A. Frizzell). The Gastrointestinal System, Intestinal absorbtion & secretion, pp. 323-336.
  7. Ding, J. L., Q. J. Sun, Y. Q. Zhu and Y. P. Zhou. 1994. Effects of dietary calcium and phosphorus on the growth and body calcium and phosphorus levels of broilers. Chinese J. Anim. Sci. 20:6-9.
  8. Han, J. C., X. D. Yang, T. Zhang, H. Li, W. L. Li, Z. Y. Zhang and J. H. Yao. 2009. Effects of 1alpha-hydroxycholecalciferol on growth performance, parameters of tibia and plasma, meat quality, and type IIb sodium phosphate cotransporter gene expression of one-to twenty-one-day-old broilers. Poult. Sci. 88:323-329. https://doi.org/10.3382/ps.2008-00252
  9. Heaney, R. P. and B. E. C. Nordin. 2002. Calcium effects on phosphorus absorption: Implications for the prevention and co-therapy of osteoporosis. J. Am. Coll. Nutr. 21:239-244. https://doi.org/10.1080/07315724.2002.10719216
  10. Hilfiker, H., I. Hattenhauer, M. Traebert, I. Forster, H. Murer and J. Biber. 1998. Characterization of a murine type II sodium-phosphate cotransporter expressed in mammalian small intestine. Proc. Natl. Acad. Sci. USA. 95:14564-14569. https://doi.org/10.1073/pnas.95.24.14564
  11. Hurwitz, S. and B. Arie. 1971. Calcium and phosphorus interrelationships in the intestine of the flow. J. Nutr. 101:677-686.
  12. Katai, K., K. Miyamoto, S. Kishida, H. Segawa, T. Nii, H. Tanaka, Y. Tani, H. Arai, S. Tatsumi, K. Morita, Y. Taketani and E. Takeda. 1999. Regulation of intestinal Na+-dependent phosphate co-transporters by a low-phosphate diet and 1,25-dihydroxyvitamin $D_3$. Biochem. J. 343:705-712. https://doi.org/10.1042/0264-6021:3430705
  13. Lambers, T. T., F. Mahieu, E. Oancea, L. Hoofd, F. Lange, A. R. Mensenkamp, T. Voets, B. Nilius, D. E. Clapham, J. G. Hoenderop and R. J. Bindels. 2006. Calbindin-D28K dynamically controls TRPV5-mediated $Ca^{2+}$ transport. EMBO J. 25:2978-2988. https://doi.org/10.1038/sj.emboj.7601186
  14. Li, J. H., J. M. Yuan, Y. M. Guo, Y. Yang, S. D. Bun, X. F. Hu and D. Yi. 2011. The effect of dietary nutrient density on growth performance, physiological parameters, and small intestinal type IIb sodium phosphate co-transporter expression in broilers. J. Anim. Sci. Biotech. 2:102-110.
  15. Masuyama, R., Y. Nakaya, S. Katsumata, Y. Kajita, M. Uehara, S. Tanaka, A. Sakai, S. Kato, T. Nakamura and K. Suzuki. 2003. Dietary calcium and phosphorus ratio regulates bone mineralization and turnover in vitamin D receptor knockout mice by affecting intestinal calcium and phosphorus absorption. J. Bone Miner. Res. 18:1217-1226. https://doi.org/10.1359/jbmr.2003.18.7.1217
  16. Quamme, G. A. 1985. Phosphate transport in intestinal brush-border membrane vesicles: Effect of pH and dietary phosphate. Am. J. Physiol. Gastrointest. Liver Physiol. 12:G168-G176.
  17. Radanovic, T., C. A. Wagner, H. Murer and J. Biber. 2005. Regulation of intestinal phosphate transport I. Segmental expression and adaptation to low-Pi diet of the type IIb Na+-Pi cotransporter in mouse small intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 288:G496-G500. https://doi.org/10.1152/ajpgi.00167.2004
  18. Rama Rao, S. V., M. V. L. N. Raju, M. R. Reddy, P. Pavani, G. Shyam Sunder and R. P. Sharma. 2003. Dietary calcium and non-phytin phosphorus interaction on growth, bone mineralization and mineral retention in broiler starter chicks. Asian-Aust. J. Anim. Sci. 16:719-725. https://doi.org/10.5713/ajas.2003.719
  19. Saddoris, K. L., J. C. Fleet and J. S. Radcliffe. 2010. Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration. J. Nutr. 140:731-736. https://doi.org/10.3945/jn.109.110080
  20. Shirley, R. B., A. J. Davis, M. M. Compton and W. D. Berry. 2003. The expression of calbindin in chicks that are divergently selected for low or high incidence of tibial dyschondroplasia. Poult. Sci. 82:1965-1973. https://doi.org/10.1093/ps/82.12.1965
  21. Wang, J. J., J. R. Wang, Z. L. Fu, P. Lou and H. Ren. 2010. Effects of dietary calcium and phosphorus levels on bone growth in broilers from 1 to 3 weeks of age. Chinese J. Anim. Nutr. 22:1088-1095.
  22. Wang, Z. R., Tania. Archbold, C. B. Yang and M. Z. Fan. 2008. Dietary true digestible calcium to phosphorus ratio affects phosphorus utilization and renal sodium and phosphate cotransporter gene expressions in post-weaned pigs. FASEB J. 22(Meeting Abstract Supplement):691-695. https://doi.org/10.1096/fj.07-9532com
  23. Xie, M., S. X. Wang, S. S. Hou and W. Huang. 2009. Interaction between dietary calcium and non-phytate phosphorus on growth performance and bone ash in early White Pekin Ducklings. Anim. Feed Sci. Technol. 151:161-166. https://doi.org/10.1016/j.anifeedsci.2009.01.005

Cited by

  1. Improvements in growth performance, bone mineral status and nutrient digestibility in pigs following the dietary inclusion of phytase are accompanied by modifications in intestinal nutrient transporter gene expression vol.112, pp.05, 2014, https://doi.org/10.1017/S0007114514001494
  2. Effect of calcium level and phytase addition on ileal phytate degradation and amino acid digestibility of broilers fed corn-based diets1 vol.93, pp.4, 2014, https://doi.org/10.3382/ps.2013-03465
  3. Effect of Dietary Nutrient Density on Small Intestinal Phosphate Transport and Bone Mineralization of Broilers during the Growing Period vol.11, pp.4, 2016, https://doi.org/10.1371/journal.pone.0153859
  4. Effects of calcium to non-phytate phosphorus ratio and different sources of vitamin D on growth performance and bone mineralization in broiler chickens vol.45, pp.1, 2016, https://doi.org/10.1590/S1806-92902016000100001
  5. High stocking density alters bone-related calcium and phosphorus metabolism by changing intestinal absorption in broiler chickens vol.97, pp.1, 2017, https://doi.org/10.3382/ps/pex294
  6. Lower dietary phosphorus supply in pigs match both animal welfare aspects and resource efficiency vol.47, pp.S1, 2018, https://doi.org/10.1007/s13280-017-0969-8
  7. 1α-Hydroxycholecalciferol improves the growth performance and up-regulates the mRNA expression of vitamin D receptor in the small intestine and kidney of broiler chickens vol.97, pp.4, 2018, https://doi.org/10.3382/ps/pex423
  8. Age, phosphorus, and 25-hydroxycholecalciferol regulate mRNA expression of vitamin D receptor and sodium-phosphate cotransporter in the small intestine of broiler chickens vol.97, pp.4, 2018, https://doi.org/10.3382/ps/pex407
  9. Dietary supplemental vitamin D3 enhances phosphorus absorption and utilisation by regulating gene expression of related phosphate transporters in the small intestine of broilers pp.1475-2662, 2018, https://doi.org/10.1017/S0007114518002763
  10. Dietary phytase supplementation during peak egg laying cycle of White Leghorn hens on nutrient utilization and functional gene mRNA expression in duodenum and kidney pp.1744-4179, 2019, https://doi.org/10.1080/09291016.2018.1499220
  11. Effects of Dietary Phosphorus Level on the Expression of Calcium and Phosphorus Transporters in Laying Hens vol.9, pp.1664-042X, 2018, https://doi.org/10.3389/fphys.2018.00627
  12. Phosphorus absorption and gene expression levels of related transporters in the small intestine of broilers vol.119, pp.12, 2018, https://doi.org/10.1017/S0007114518000934
  13. Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens vol.4, pp.None, 2012, https://doi.org/10.1186/2049-1891-4-34
  14. Reduction of calcium levels in rations supplemented with vitamin D3 or 25-OH-D3 for broilers vol.48, pp.None, 2019, https://doi.org/10.1590/rbz4820180253
  15. Available Phosphorus and Calcium Reduction in the Finisher Phase and Phytase Utilization on Broilers vol.28, pp.2, 2012, https://doi.org/10.3382/japr/pfy066
  16. Evaluation of Struvite Recovered from Swine Wastewater as an Alternative Phosphorus Source in Broiler Feed vol.9, pp.10, 2012, https://doi.org/10.3390/agriculture9100221
  17. Efficacy of 1-α-Hydroxycholecalciferol Supplementation in Young Broiler Feed Suggests Reducing Calcium Levels at Grower Phase vol.7, pp.None, 2012, https://doi.org/10.3389/fvets.2020.00245
  18. Dietary calcium and meat and bone meal as potential precursors for the onset of necrotic enteritis vol.76, pp.4, 2020, https://doi.org/10.1080/00439339.2020.1831419
  19. Requirement of digestible calcium at different dietary concentrations of digestible phosphorus for broiler chickens. 1. Broiler starters (d 1 to 10 post-hatch) vol.100, pp.11, 2012, https://doi.org/10.1016/j.psj.2021.101439
  20. Transcriptional responses in jejunum of two layer chicken strains following variations in dietary calcium and phosphorus levels vol.22, pp.1, 2012, https://doi.org/10.1186/s12864-021-07814-9
  21. Growth, physiological, and molecular responses of broiler quail to dietary source, particle size, and choice feeding of calcium vol.21, pp.1, 2022, https://doi.org/10.1080/1828051x.2021.2017361