Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

Feeder Independent Culture of Mouse Embryonic Stem Cells

  • Kim, Myoung Ok (School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Ryoo, Zae Young (School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University)
  • Received : 2012.12.21
  • Accepted : 2012.12.24
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.

Keywords

References

  1. Amit M, Shariki C, Margulets V, Itskovitz-Eldor J (2004): Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 70:837-45.
  2. Berger MG, Veyrat-Masson R, Rapatel C, Descamps S, Chassagne J, Boiret-Dupre N (2006): Cell culture medium composition and translational adult bone marrow- derived stem cell research. Stem Cells 24:2888-2890. https://doi.org/10.1634/stemcells.2006-0387
  3. Evans MJ, Kaufman MH (1981): Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154-156. https://doi.org/10.1038/292154a0
  4. Halme DG, Kessler DA (2006): FDA regulation of stem-cell-based therapies. N Engl J Med, 355:1730-5. https://doi.org/10.1056/NEJMhpr063086
  5. Javazon EH, Beggs KJ, Flake AW (2004): Mesenchymal stem cells: paradoxes of passaging. Exp Hematol 32:414-425. https://doi.org/10.1016/j.exphem.2004.02.004
  6. Kim HT, Lee K, Kim DW, Hwang DY (2013): An ECMbased culture system for the generation and maintenance of xeno-free human iPS cells. Biomaterials 34:1041-1050. https://doi.org/10.1016/j.biomaterials.2012.10.064
  7. Kim JH, Auerbach JM, Rodriguez-Gomez JA, Velasco I, Gavin D, Lumelsky N, Lee SH, Nguyen J, Sanchez- Pernaute R, Bankiewicz K, Mckay R (2002): Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease. Nature 418:50-56. https://doi.org/10.1038/nature00900
  8. Lagasse E, Shizuru JA, Uchida N, Tsukamoto A, Weissman IL (2001): Toward regenerative medicine. Immunity, 14:425-436. https://doi.org/10.1016/S1074-7613(01)00123-6
  9. Li Y, Powell S, Brunette E, Lebkowski J, Mandalam R (2005): Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products. Biotechnol Bioeng 91:688-698. https://doi.org/10.1002/bit.20536
  10. Mannello F, Tonti GA (2007): Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold!. Stem Cells 25: 1603-1609. https://doi.org/10.1634/stemcells.2007-0127
  11. Shahdadfar A, Fronsdal K, Haug T, Reinholt FP, Brinchmann JE (2005): In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells 23: 1357-1366. https://doi.org/10.1634/stemcells.2005-0094
  12. Smith AG (2001): Embryo-derived stem cells: of mice and men. Annu Rev Cell Dev Biol 17:435-62. https://doi.org/10.1146/annurev.cellbio.17.1.435
  13. Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S (1993): A novel ES cell line, TT2, with high germline- differentiating potency. Anal Biochem 214:70-76. https://doi.org/10.1006/abio.1993.1458