Reproductive and Developmental Biology

  • 제36권4호
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  • Pages.249-254
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  • 2012
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Effect of Porcine Follicular Fluid on Donor Cell Characteristics and Quality of Porcine Cloned Blastocysts

  • Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Jeong Woong (Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sung-Soo (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Chang, Won-Kyong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 투고 : 2012.11.08
  • 심사 : 2012.12.14
  • 발행 : 2012.12.31
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초록

This study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the characteristics of donor cells and the developmental competence of porcine cloned embryos. Ear fibroblast cells (donor cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% FBS (SS); and (3) DMEM+10% FBS+10% pFF (pFF) for 72 h. In each conditioned medium, the concentrations of 4 amino acids (Thr, Glu, Pro, and Val) in the pFF group were significantly different from those in the control group (p<0.05 or p<0.01). The proliferation of the cells cultured in the SS group was significantly lower than that of the other treatment groups (p<0.01). The population of apoptotic and necrotic cells in the SS group was significantly higher than that of either the control or the pFF group (p<0.01). The number of embryos that cleaved (p<0.05) and developed into blastocysts (p<0.01) in the SS group was significantly lower than that of either the control or the pFF group. Compared to other groups, the blastocysts produced from the donor cells in the pFF group had higher total cells and lower apoptotic cells (p<0.05). It can be concluded that pFF supplementation in the donor cell culture medium positively affects cell death, cell cycle and quality of the cloned blastocyst.

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참고문헌

  1. Abeydeera LR, Wang WH, Cantley TC, Rieke A, Murphy CN, Prather RS, Day BN (2000): Development and viability of pig oocytes matured in a protein- free medium containing epidermal growth factor. Theriogenology 15:787-797.
  2. Ahn KS, Kim YJ, Kim M, Lee BH, Heo SY, Kang MJ, Kang YK, Lee JW, Lee KK, Kim JH, Nho WG, Hwang SS, Woo JS, Park JK, Park SB, Shim H (2011): Resurrection of an alpha-1,3-galactosyltransferase gene-targeted miniature pig by recloning using postmortem ear skin fibroblasts. Theriogenology 75:933-939. https://doi.org/10.1016/j.theriogenology.2010.11.001
  3. Algriany O, Bevers M, Schoevers E, Colenbrander B, Dieleman S (2004): Follicle size-dependent effects of sow follicular fluid on in vitro cumulus expansion, nuclear maturation and blastocyst formation of sow cumulus oocytes complexes. Theriogenology 62:1483-1497. https://doi.org/10.1016/j.theriogenology.2004.02.008
  4. Byrne AT, Southgate J, Brison DR, Leese HJ (1999): Analysis of apoptosis in the pre-implantation bovine embryo using TUNEL. J Reprod Fertil 117:97-105. https://doi.org/10.1530/jrf.0.1170097
  5. Dode MA, Graves C (2002): Involvement of steroid hormones on in vitro maturation of pig oocytes. Theriogenology 15:811-821.
  6. Egerszegi I, Alm H, Rátky J, Heleil B, Brüssow KP, Torner H (2010): Meiotic progression, mitochondrial features and fertilisation characteristics of porcine oocytes with different G6PDH activities. Reprod Fertil Dev 22:830-838. https://doi.org/10.1071/RD09140
  7. Engelen MP, Schols AM, Does JD, Deutz NE, Wouters EF (2000): Altered glutamate metabolism is associated with reduced muscle glutathione levels in patients with emphysema. Am J Respir Crit Care Med 161:98-103. https://doi.org/10.1164/ajrccm.161.1.9901031
  8. Gorman L, Mercer LP, Hennig B (1996): Growth requirements of endothelial cells in culture: Variations in serum and amino acid concentrations. Nutrition 12:266-270 https://doi.org/10.1016/S0899-9007(96)90854-0
  9. Hong J, Lee E (2007): Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocyte ma turation, fertilization, and preimplantation development. Theriogenology 68:728-735. https://doi.org/10.1016/j.theriogenology.2007.06.002
  10. Hong JY, Yong HY, Lee BC, Hwang WS, Lim JM, Lee ES (2004): Effects of amino acids on maturation, fertilization and embryo development of pig follicular oocytes in two IVM media. Theriogenology 62:1473-1482. https://doi.org/10.1016/j.theriogenology.2004.02.013
  11. Im GS, Samuel M, Lai L, Hao Y, Prather RS (2007): Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Mol Reprod Dev 74:1158-1164. https://doi.org/10.1002/mrd.20492
  12. Iwata H, Hashimoto S, Ohota M, Kimura K, Shibano K, Miyake M (2004): Effects of follicle size and electrolytes and glucose in maturation medium on nuclear maturation and developmental competence of bovine oocytes. Reproduction 127:159-164. https://doi.org/10.1530/rep.1.00084
  13. Jarkovska K, Martinkova J, Liskova L, Halada P, Moos J, Rezabek K, Gadher SJ, Kovarova H (2010): Proteome mining of human follicular fluid reveals a crucial role of complement cascade and key biological pathways in women undergoing in vitro fertilization. J Proteome Res 9:1289-1301. https://doi.org/10.1021/pr900802u
  14. Jurisicova A, Latham KE, Casper RF, Varmuza SL (1998): Expression and regulation of genes associated with cell death during murine pre-implantation embryo development. Mol Reprod Dev 51:243-253. https://doi.org/10.1002/(SICI)1098-2795(199811)51:3<243::AID-MRD3>3.0.CO;2-P
  15. Klose R, Kemter E, Bedke T, Bittmann I, Kelsser B, Endres R, Pfeffer K, Schwinzer R, Wolf E (2005): Expression of biologically active human TRAIL in transgenic pigs. Transplantation 80:222-230. https://doi.org/10.1097/01.TP.0000164817.59006.C2
  16. Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS (2002): Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295:1089-1092. https://doi.org/10.1126/science.1068228
  17. Lédée N, Lombroso R, Lombardelli L, Selva J, Dubanchet S, Chaouat G, Frankenne F, Foidart JM, Maggi E, Romagnani S, Ville Y, Piccinni MP (2008): Cytokines and chemokines in follicular fluids and potential of the corresponding embryo: the role of granulocyte colony-stimulating factor. Hum Reprod 23:2001-2009. https://doi.org/10.1093/humrep/den192
  18. Metoki T, Iwata H, Itoh M, Kasai M, Takajyo A, Suzuki A, Kuwayama T, Monji Y (2008): Effects of follicular fluids on the growth of porcine preantral follicle and oocyte. Zygote 16:239-247. https://doi.org/10.1017/S0967199408004711
  19. Min SH, Park H (2012): Effects of thymidine on in vitro maturation of immature porcine follicular oocytes. Reprod Dev Biol 36:71-78.
  20. Murakami H, Nagashima H, Takahagi Y, Miyagawa S, Fujimura T, Toyomura K, Nakai R, Yamada M, Kurihara T, Shigehisa T, Okabe M, Seya T, Shirakura R, Kinoshita T (2002): Transgenic pigs expressing human decay-accelerating factor regulated by porcine MCP gene promoter. Mol Reprod Dev 61:302-311. https://doi.org/10.1002/mrd.10043
  21. Nagashima H, Fujimura T, Takahagi Y, Kurome M, Wako N, Ochiai T, Esaki R, Kano K, Saito S, Okabe M, Murakami H (2003) Development of efficient strategies for the production of genetically modified pigs. Theriogenology 59: 95-106. https://doi.org/10.1016/S0093-691X(02)01261-X
  22. Nakazawa T, Ohashi K, Yamada M, Shinoda S, Saji F, Murat Y, Araki H (1997): Effect of different concentrations of amino acids in human serum and follicular fluid on the development of one-cell mouse embryos in vitro. J Reprod Fertil 111:327-332. https://doi.org/10.1530/jrf.0.1110327
  23. Park JH, Chung YH, Rhee MH, Kim SK (2012): Effect of activation of porcine NT embryos on in vitro development. Reprod Dev Biol 35:475-478.
  24. Park JK, Lee YK, Lee P, Chung HJ, Kim S, Lee HG, Seo MK, Han JH, Park CG, Kim HT, Kim YK, Min SK, Kim JH, Lee HT, Chang WK (2006): Recombinant human erythropoietin produced in milk of transgenic pigs. J Biotechnology 122:362-371. https://doi.org/10.1016/j.jbiotec.2005.11.021
  25. Shim JH, Park MR, Yang BC, Ko YG, Oh KB, Lee JW, Woo JS, Park EW, Park SB, Hwang S (2009): Developmental characteristics of SCNT pig embryos knocked-out of alpha-1,3-galactosyltransferase gene. Reprod Dev Biol 33:157-162.
  26. Stice SL, Gibbons J, Rzucidlo JR, Baile CA (2000): Improvements in nuclear transfer procedures will increase commercial utilization of animal cloning. Asian Aust J Anim Sci 13, 856-860.
  27. Takahagi Y, Fujimura T, Miyagawa S, Nagashima H, Shigehisa T, Shirakura R, Murakami H (2005): Production of alpha 1,3-galactosyltransferase gene knockout pigs expressing both human decay-accelerating factor and Nacetylglucosaminyltransferase III. Mol Reprod Dev 71:331-338. https://doi.org/10.1002/mrd.20305
  28. van der Valk J, Brunner D, De Smet D, Svenningsen AF, Honegger P, Knudsen LE, Lindl T, Noraberg J, Price A, Scarino ML, Gstraunthaler G (2010). Optimization of chemically defined cell culture media - Replacing fetal bovine serum in mammalian in vitro methods. Toxicology in Vitro 24:1053-1063. https://doi.org/10.1016/j.tiv.2010.03.016
  29. White JA, Hart RJ, Fly JC (1988): An evaluation of waters pico-tag system for the amino acid analysis of food materials. J Automatic Chem 8:170-177.
  30. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385: 810-813. https://doi.org/10.1038/385810a0
  31. Yoon KW, Shin TY, Park JI, Roh S, Lim JS, Lee BC, Hwang WS, Lee ES (2000): Development of porcine oocytes from preovulatory follicles of different sizes after maturation in media supplemented with follicular fluids. Reprod Fertil Dev 12:133-139. https://doi.org/10.1071/RD00027