Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Experimental Murine Fascioliasis Derives Early Immune Suppression with Increased Levels of TGF-${\beta}$ and IL-4

  • Chung, Joon-Yong (Tissue Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, NIH) ;
  • Bae, Young-An (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Centers for Molecular Medicine, Samsung Biomedical Research Institute) ;
  • Yun, Doo-Hee (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Centers for Molecular Medicine, Samsung Biomedical Research Institute) ;
  • Yang, Hyun-Jong (Department of Parasitology, Ewha Womans University School of Medicine) ;
  • Kong, Yoon (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Centers for Molecular Medicine, Samsung Biomedical Research Institute)
  • Received : 2012.03.29
  • Accepted : 2012.07.05
  • Published : 2012.12.17
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Abstract

In fascioliasis, T-helper 2 (Th2) responses predominate, while little is known regarding early immune phenomenon. We herein analyzed early immunophenotype changes of BALB/c, C57BL/6, and C3H/He mice experimentally infected with 5 Fasciola hepatica metacercariae. A remarkable expansion of $CD19^+$ B cells was observed as early as week 1 post-infection while $CD4^+/CD8^+$ T cells were down-regulated. Accumulation of $Mac1^+$ cells with time after infection correlated well with splenomegaly of all mice strains tested. The expression of tumor necrosis factor (TNF)-${\alpha}$ mRNA in splenocytes significantly decreased while that of IL-4 up-regulated. IL-$1{\beta}$ expression was down-modulated in BALB/c and C57BL/6 mice, but not in C3H/He. Serum levels of transforming growth factor (TGF)-${\beta}$ were considerably elevated in all mice during 3 weeks of infection period. These collective results suggest that experimental murine fascioliasis might derive immune suppression with elevated levels of TGF-${\beta}$ and IL-4 during the early stages of infection.

Keywords

References

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