Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Changes in periodontium after extraction of a periodontally-involved tooth in rats

  • Kim, Dong-Ju (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Cha, Jae-Kook (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Yang, Cheryl (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Cho, Ahran (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Lee, Jung-Seok (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Jung, Ui-Won (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Kim, Chang-Sung (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Lee, Seung-Jong (Department of Conservative Dentistry, Yonsei University College of Dentistry) ;
  • Choi, Seong-Ho (Department of Periodontology, Yonsei University College of Dentistry)
  • Received : 2012.06.13
  • Accepted : 2012.10.04
  • Published : 2012.10.31
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Abstract

Purpose: Recent interest has focused on intentional replantation to restore an original tooth. Some studies have shown successful results with intentional replantation for periodontally involved teeth. For long-term success of replantation, a healthy periodontal status of the recipient site is required so that delayed replantation is more suitable for periodontally involved teeth. To reveal the ideal timing for delayed replantation of periodontally involved teeth, the healing process of extraction sockets after extraction of periodontitis-induced teeth in rats was evaluated. Methods: Twenty-eight rats were randomly divided into two groups: a control group (n=8) and test group (n=20). In the test group, periodontitis was induced by a ligature around the cervix of the mandibular first molar of all of the rats. Two weeks later, the mandibular first molars were extracted in all of the animals. The animals were sacrificed on days 0, 3, 7, and 10 after extraction and histological and immunohistochemical analysis was performed. Results: In histological analysis of the test group, inflammatory cell infiltrate was found abundantly in the remaining periodontium 3 days after tooth extraction and decreased gradually at later time points. In immunohistochemical analysis of the test group, both interleukin-6 (IL-6) and, tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) were numerous in the furcation area at each postextraction day. IL-6 was stained more heavily between 3 and 7 days after extraction; at day 10 after extraction, little staining was observed. TNF-${\alpha}$ staining was more intense at 3 days after extraction and gradually weakened at later points in time. Conclusions: Within the limits of this study, it takes at least 10 days to resolve periodontal inflammation in rat extraction sockets.

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References

  1. Grossman LI. Intentional replantation of teeth: a clinical evaluation. J Am Dent Assoc 1982;104:633-9. https://doi.org/10.14219/jada.archive.1982.0252
  2. Lu DP. Intentional replantation of periodontally involved and endodontically mistreated tooth. Oral Surg Oral Med Oral Pathol 1986;61:508-13. https://doi.org/10.1016/0030-4220(86)90396-8
  3. Demiralp B, Nohutcu RM, Tepe DI, Eratalay K. Intentional replantation for periodontally involved hopeless teeth. Dent Traumatol 2003;19:45-51. https://doi.org/10.1034/j.1600-9657.2003.00084.x
  4. Hammarstrom L, Blomlof L, Lindskog S. Dynamics of dentoalveolar ankylosis and associated root resorption. Endod Dent Traumatol 1989;5:163-75. https://doi.org/10.1111/j.1600-9657.1989.tb00354.x
  5. Andreasen JO. Analysis of pathogenesis and topography of replacement root resorption (ankylosis) after replantation of mature permanent incisors in monkeys. Swed Dent J 1980;4:231-40.
  6. Kratchman S. Intentional replantation. Dent Clin North Am 1997;41:603-17.
  7. Dryden JA, Arens DE. Intentional replantation. A viable alternative for selected cases. Dent Clin North Am 1994;38: 325-53.
  8. Sharma NK, Duggal MS. Replantation in general dental practice. Br Dent J 1994;176:147-51. https://doi.org/10.1038/sj.bdj.4808392
  9. Messkoub M. Intentional replantation: a successful alternative for hopeless teeth. Oral Surg Oral Med Oral Pathol 1991;71:743-7. https://doi.org/10.1016/0030-4220(91)90286-L
  10. Lindskog S, Pierce AM, Blomlof L, Hammarstrom L. The role of the necrotic periodontal membrane in cementum resorption and ankylosis. Endod Dent Traumatol 1985;1: 96-101. https://doi.org/10.1111/j.1600-9657.1985.tb00569.x
  11. Mahajan SK, Sidhu SS. Periodontal ligament, extra-oral period and use of fluorides in replantation of teeth. Indian J Med Res 1982;75:441-5.
  12. Madison S. Intentional replantation. Oral Surg Oral Med Oral Pathol 1986;62:707-9. https://doi.org/10.1016/0030-4220(86)90268-9
  13. Nethander G, Skoglund A, Kahnberg KE. Experimental autogenous tooth transplantation in the dog: a comparison between one- and two-stage surgical techniques. Acta Odontol Scand 2003;61:223-9. https://doi.org/10.1080/00016350310004098
  14. Duarte PM, Tezolin KR, Figueiredo LC, Feres M, Bastos MF. Microbial profile of ligature-induced periodontitis in rats. Arch Oral Biol 2010;55:142-7. https://doi.org/10.1016/j.archoralbio.2009.10.006
  15. Chilosi M, Mombello A, Montagna L, Benedetti A, Lestani M, Semenzato G, et al. Multimarker immunohistochemical staining of calgranulins, chloroacetate esterase, and S100 for simultaneous demonstration of inflammatory cells on paraffin sections. J Histochem Cytochem 1990;38: 1669-75. https://doi.org/10.1177/38.11.2212622
  16. Pulford KA, Sipos A, Cordell JL, Stross WP, Mason DY. Dis tribution of the CD68 macrophage/myeloid associated antigen. Int Immunol 1990;2:973-80. https://doi.org/10.1093/intimm/2.10.973
  17. Ackermann MR, DeBey BM, Stabel TJ, Gold JH, Register KB, Meehan JT. Distribution of anti-CD68 (EBM11) immunoreactivity in formalin-fixed, paraffin-embedded bovine tissues. Vet Pathol 1994;31:340-8. https://doi.org/10.1177/030098589403100307
  18. Kornman KS, Page RC, Tonetti MS. The host response to the microbial challenge in periodontitis: assembling the players. Periodontol 2000 1997;14:33-53. https://doi.org/10.1111/j.1600-0757.1997.tb00191.x
  19. Page RC, Offenbacher S, Schroeder HE, Seymour GJ, Kornman KS. Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions. Periodontol 2000 1997;14:216-48. https://doi.org/10.1111/j.1600-0757.1997.tb00199.x
  20. Todo H. Healing mechanism of tooth extraction wounds in rats. I. Initial cellular response to tooth extraction in rats studied with 3H-thymidine. Arch Oral Biol 1968;13:1421-7. https://doi.org/10.1016/0003-9969(68)90024-1
  21. Sato H, Takeda Y. Proliferative activity, apoptosis, and histogenesis in the early stages of rat tooth extraction wound healing. Cells Tissues Organs 2007;186:104-11. https://doi.org/10.1159/000103513
  22. Baker PJ, Dixon M, Evans RT, Dufour L, Johnson E, Roopenian DC. CD4(+) T cells and the proinflammatory cytokines gamma interferon and interleukin-6 contribute to alveolar bone loss in mice. Infect Immun 1999;67:2804-9.
  23. Borch TS, Holmstrup P, Bendtzen K, Nielsen CH. In vitro cytokine responses to periodontal pathogens: generalized aggressive periodontitis is associated with increased IL-6 response to Porphyromonas gingivalis. Scand J Immunol 2010;71:440-6. https://doi.org/10.1111/j.1365-3083.2010.02390.x
  24. Tonetti MS, D'Aiuto F, Nibali L, Donald A, Storry C, Parkar M, et al. Treatment of periodontitis and endothelial function. N Engl J Med 2007;356:911-20. https://doi.org/10.1056/NEJMoa063186
  25. Lopez Carriches C, Martinez Gonzalez JM, Donado Rodriguez M. Variations of interleukin-6 after surgical removal of lower third molars. Med Oral Patol Oral Cir Bucal 2006; 11:E520-6.
  26. Miyawaki T, Maeda S, Shimada M. Elevation of plasma interleukin-6 level in patients undergoing oral and maxillofacial surgery. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1996;81:15-20. https://doi.org/10.1016/S1079-2104(96)80140-5
  27. Cruickshank AM, Fraser WD, Burns HJ, Van Damme J, Shenkin A. Response of serum interleukin-6 in patients undergoing elective surgery of varying severity. Clin Sci (Lond) 1990;79:161-5. https://doi.org/10.1042/cs0790161
  28. Graves DT, Oskoui M, Volejnikova S, Naguib G, Cai S, Desta T, et al. Tumor necrosis factor modulates fibroblast apoptosis, PMN recruitment, and osteoclast formation in response to P. gingivalis infection. J Dent Res 2001;80:1875-9. https://doi.org/10.1177/00220345010800100301
  29. Gaspersic R, Stiblar-Martincic D, Osredkar J, Skaleric U. Influence of subcutaneous administration of recombinant TNF-alpha on ligature-induced periodontitis in rats. J Periodontal Res 2003;38:198-203. https://doi.org/10.1034/j.1600-0765.2003.01395.x
  30. Garlet GP, Cardoso CR, Campanelli AP, Ferreira BR, Avila-Campos MJ, Cunha FQ, et al. The dual role of p55 tumour necrosis factor-alpha receptor in Actinobacillus actinomycetemcomitans-induced experimental periodontitis: host protection and tissue destruction. Clin Exp Immunol 2007;147:128-38.
  31. Liu R, Bal HS, Desta T, Krothapalli N, Alyassi M, Luan Q, et al. Diabetes enhances periodontal bone loss through enhanced resorption and diminished bone formation. J Dent Res 2006;85:510-4. https://doi.org/10.1177/154405910608500606

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