Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Cyclooxygenase 2 gene polymorphisms and chronic periodontitis in a North Indian population: a pilot study

  • Daing, Anika (Department of Periodontics, Faculty of Dental Sciences, Chhatrapati Shahuji Maharaj Medical University) ;
  • Singh, Sarvendra Vikram (Genotoxicity Laboratory, Toxicology Division, CSIR-Central Drug Research Institute) ;
  • Saimbi, Charanjeet Singh (Department of Periodontics, Faculty of Dental Sciences, Chhatrapati Shahuji Maharaj Medical University) ;
  • Khan, Mohammad Akhlaq (Department of Periodontics, Faculty of Dental Sciences, Chhatrapati Shahuji Maharaj Medical University) ;
  • Rath, Srikanta Kumar (Genotoxicity Laboratory, Toxicology Division, CSIR-Central Drug Research Institute)
  • Received : 2012.06.06
  • Accepted : 2012.10.04
  • Published : 2012.10.31
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Abstract

Purpose: Cyclooxygenase (COX) enzyme catalyzes the production of prostaglandins, which are important mediators of tissue destruction in periodontitis. Single nucleotide polymorphisms of $COX_2$ enzyme have been associated with increasing susceptibility to inflammatory diseases. The present study evaluates the association of two single nucleotide polymorphisms in $COX_2$ gene (-1195G>A and $8_{473}$C>T) with chronic periodontitis in North Indians. Methods: Both SNPs and their haplotypes were used to explore the associations between $COX_2$ polymorphisms and chronic periodontitis in 56 patients and 60 controls. Genotyping was done by polymerase chain reaction followed by restriction fragment length polymorphism. Chi-square test and logistic regression analysis were performed for association analysis. Results: By the individual genotype analysis, mutant genotypes (GA and AA) of $COX_2$-1195 showed more than a two fold risk (odds ratio [OR]>2) and $COX_2$ $8_{473}$ (TC and CC) showed a reduced risk for the disease, but the findings were not statistically significant. Haplotype analysis showed that the frequency of the haplotype AT was higher in the case group and a significant association was found for haplotype AT (OR, 1.79; 95% confidence interval, 1.03 to 3.11; P=0.0370) indicating an association between the AT haplotype of $COX_2$ gene SNPs and chronic periodontitis. Conclusions: Individual genotypes of both the SNPs were not associated while haplotype AT was found to be associated with chronic periodontitis in North Indians.

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References

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