오적산의 추출 용매 차이에 따른 염증 및 염증 관련 질환에 대한 효능 비교

Effects of water and ethanol extracts from Ojeok-san on inflammation and its related diseases

  • 유새롬 (한국한의학연구원 한약기초연구그룹) ;
  • 정수진 (한국한의학연구원 한약기초연구그룹) ;
  • 김예지 (한국한의학연구원 한약기초연구그룹) ;
  • 임혜선 (한국한의학연구원 한약기초연구그룹) ;
  • 진성은 (한국한의학연구원 한약기초연구그룹) ;
  • 전우영 (한국한의학연구원 한약기초연구그룹) ;
  • 신인식 (한국한의학연구원 한약기초연구그룹) ;
  • 신나라 (한국한의학연구원 한약기초연구그룹) ;
  • 김성실 (한국한의학연구원 한약기초연구그룹) ;
  • 김정훈 (한국한의학연구원 한약기초연구그룹) ;
  • 하혜경 (한국한의학연구원 한약기초연구그룹) ;
  • 이미영 (한국한의학연구원 한약기초연구그룹) ;
  • 김온순 (한국한의학연구원 한약기초연구그룹) ;
  • 서창섭 (한국한의학연구원 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원 한약기초연구그룹)
  • Yoo, Sae-Rom (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jeong, Soo-Jin (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Ye-Ji (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lim, Hye-Sun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jin, Seong-Eun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jeon, Woo-Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, In-Sik (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Na-Ra (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Seong-Sil (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Mee-Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Ohn-Soon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 발행 : 2012.12.30

초록

Objectives : Ojeok-san, a traditional herbal formula, has been used for the treatment of cold illness and its related symptoms such as headache, nausea and indigestion. This study was performed to compare effects of water (OJSW) and 70% ethanol extracts (OJSE) of Ojeok-san on inflammation and its related diseases atopy, asthma and obesity in vitro. Methods : We performed HPLC to investigate contents of index components of OJSW and OJSE. We investigated the effects of OJSW and OJSE with an in vitro model, using 5 cell lines, specifically RAW 264. 7, HaCaT, MC/9, BEAS-2B and 3T3-L1. Results : HPLC analysis displayed that the contents of index components were higher in OJSE than OJSW. In lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, OJSE significantly inhibited productions of interleukin (IL)-6, nitrite and prostaglandin $E_2$ ($PEG_2$). In TNF-${\alpha}$/IFN-${\gamma}$-treated HaCaT keratinocytes, OJSE significantly lowered levels of macrophage-derived chemokine (MDC) as well as regulated and normal T cell expressed and secreted (RANTES). OJSE also had a protective effect on inflammatory response by decreasing RANTES secretion in TNF-${\alpha}$-stimulated BEAS-2B cells. Conclusions : We conclude that OJSE could be more appropriate to enhance the biological activities against inflammation and its related diseases, and could be applied as a bioactive material for developing the potent anti-inflammatory agents.

키워드

참고문헌

  1. Sung YY, Kim SD, Yang WK, Nho KJ, Seo HS, Kim YS, et al. Inhibitory effects of Drynaria fortunei extract on house dust mite antigen-induced atopic dermatitis in NC/Nga mice. J Ethnopharmacol 2012 [in press].
  2. 한의과대학 방제학교수 공편저. 방제학-개정증보-. 서울: 영림사; 2003, p. 268
  3. 보건복지부. 한방의료 이용 및 한약소비실태조사. 보건복지부 한국보건사회연구원; 2011, p. 38-9.
  4. 전미혜, 백승희, 신상우, 이영선. 오적산이 자궁 근종세 세포의 사멸과 cell apoptosis에 미치는 영향. 대한한방부인과학회지 2003;16(2):45-55.
  5. 김재형, 소경순, 정찬길, 김광호. 오적산이 흰쥐의 고지혈증에 미치는 영향에 대한 실험적 연구. 대한예방한의학회지 2004;8(2):185-202.
  6. 박종기, 채우석. 오적산 엑기스 과립의 경구투여와 수침이 동통에 미치는 영향. 대한침구학회지 1992;9(10):73-91.
  7. 정다영, 하혜경, 이호영, 이진아, 이준경, 황대선, 등. 대표적인 風寒熱에 의한 통증 치료 처방의 면역 활성화 비교 연구. 동의생리병리학회지 2012;24(4):616-23.
  8. 조영제, 천성숙, 권효정, 김정환, 윤소정, 이경환. 복분자 (Rubus coreanum F.) 열수 및 에탄올추 출물의 생리활성비교. 식품영양과학회지 2005;34(6) :790-6.
  9. Posadas I, Terencio MC, Guillén I, Ferrandiz ML, Coloma J, Paya M, et al. Co-regulationbetween cyclo-oxygenase-2 and inducible nitric oxide synthaseexpression in the time-course of murine inflammation. Naunyn Schmiedebergs Arch Pharmacol 2000;361:98-106. https://doi.org/10.1007/s002109900150
  10. Balkwill F, Mantonavi A. Inflammation and cancer: back to Virchow?. Lancet 2001;357:537-45. https://doi.org/10.1016/S0140-6736(05)71691-3
  11. Lane SJ, Lee TH. Mast cell effector mechanisms. J Allergy ClinImmunol 1996;98:S67-72. https://doi.org/10.1016/S0091-6749(96)70019-2
  12. Chung KF, Barnes PJ. Cytokines in asthma. Thorax 1999;54:825-57. https://doi.org/10.1136/thx.54.9.825
  13. Imai T, Chantry D, Raport CJ, Wood CL, Nishimura M, Godiska R, et al. Macrophagederived chemokine is a functional ligand for the CC chemokine receptor. J Biol Chem 1998; 16:273(3):1764-8. https://doi.org/10.1074/jbc.273.3.1764
  14. Frank S, Kmpfer H, Wetzler C, Stallmeyer B, Pfeilschifter J. Large induction of the chemotactic cytokine RANTES during cutaneous wound repair: a regulatory role for nitric oxide in keratinocyte-derived RANTES expression. Biochem J 2000;347(1):265-73. https://doi.org/10.1042/0264-6021:3470265
  15. Xiao T, Kagami S, Saeki H, Sugaya M, Kakinuma T, Fujita H, et al. Both IL-4 and IL-13 inhibit the TNF-$\alpha$ and IFN-$\gamma$ enhanced MDC production in a human keratinocyte cell line, HaCaT cells. J Dermato Sci 2003;31(2):111-7. https://doi.org/10.1016/S0923-1811(02)00149-4
  16. Zhang M, Zhu L, Feng Y, Yang Y, Liu L, Ran Y. Effects of acitretin on proliferative inhibition and RANTES production of HaCaT cells. Arch Dermatol Res 2008;300(10):575-81. https://doi.org/10.1007/s00403-008-0872-z
  17. Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-alpha:direct role in obesity-linked insulin resistance. Science 1993;259:87-91. https://doi.org/10.1126/science.7678183
  18. Cao Z, Umek RM, McKnight SL. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes Dev 1991;5:1538-52. https://doi.org/10.1101/gad.5.9.1538
  19. Kanno S, Shouji A, Tomizawa A, Hiura T, Osanai Y, Ujibe M, et al. Inhibitory effect of naringin on lipopolysaccharide (LPS)-induced endotoxin shock in mice and nitric oxide production in RAW 264.7 macrophages. Life Sci 2006;78(7):673-81. https://doi.org/10.1016/j.lfs.2005.04.051
  20. Si-Si W, Liao L, Ling Z, Yun-Xia Y. Inhibition of TNF$\alpha$/IFN-$\gamma$ induced RANTES expression in HaCaT cell by naringin. Pharm Biol 2011; 49(8):810-4. https://doi.org/10.3109/13880209.2010.550054
  21. Kim SH, Kim BK, Lee YC. Antiasthmatic effects of hesperidin, a potential Th2 cytokine antagonist, in a mouse model of allergic asthma. Mediators Inflamm 2011: 485-02.
  22. Petrova A, Davids LM, Rautenbach F, Marnewick JL. Photoprotection by honeybush extracts, hesperidin and mangiferin against UVB-induced skin damage in SKH-1 mice. J Photochem Photobiol B 2011;103(2):126-39. https://doi.org/10.1016/j.jphotobiol.2011.02.020
  23. Wankun X, Wenzhen Y, Min Z, Weiyan Z, Huan C, Wei D, et al. Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro. Mol Vis 2011;17:3512-22.
  24. Bhattacharjee S, Bhattacharjee A, Majumder S, Majumdar SB, Majumdar S. Glycyrrhizic acid suppresses Cox-2-mediated anti-inflammatory responses during Leishmania donovani infection. J Antimicrob Chemother 2012;67(8):1905-14. https://doi.org/10.1093/jac/dks159
  25. Gwak GY, Moon TG, Lee DH, Yoo BC. Glycyrrhizin attenuates HMGB1-induced hepatocyte apoptosis by inhibiting the p38-dependent mitochondrial pathway. World J Gastroenterol 2012;21:18(7):679-84. https://doi.org/10.3748/wjg.v18.i7.679
  26. Rim HK, Cho W, Sung SH, Lee KT. Nodakenin Suppresses Lipopolysaccharide-Induced Inflammatory Responses in Macrophage cells by Inhibiting Tumor Necrosis Factor Receptor-Associated Factor 6 and Nuclear Factor-${\kappa}B$ Pathways and Protects Mice from Lethal Endotoxin Shock. World J Gastroenterol 2012;18(7):679-84. https://doi.org/10.3748/wjg.v18.i7.679
  27. Xu BJ, Chang SK. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci 2007;72(2):159-66. https://doi.org/10.1111/j.1750-3841.2006.00260.x
  28. Akowuah GA, Ismail Z, Norhayati I, Sadikun A. The effects of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radicalscavenging activity. Food Chem 2005;93:313-7.
  29. 김형석, 노정두. 육계약침액이 LPS로 유도된 대식세포의 염증반응에 미치는 영향. 대한침구학회지 2012;29(1):15-24.
  30. 윤경진, 이은용. 금은화 추출액이 Raw 264.7 macrophage에서의 NO와 PGE2 생성에 미치는 영향. 대한침구학회지 2012;29(1):67-74.