Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Baked Garlic Powder on Lipid Metabolism in Rats Fed a High-Fat/High-Cholesterol Diet

구운 마늘 분말이 고지방-고콜레스테롤 식이를 급여한 흰쥐의 체내 지질대사에 미치는 효과

  • Lee, Oun-Ju (Dept. of Nutrition and Culinary Science, Hankyoung National University) ;
  • Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University) ;
  • Lee, Myung-Yul (Dept. of Food and Nutrition, Chosun University) ;
  • Lee, Hyun-Joo (Dept. of Nutrition and Culinary Science, Hankyoung National University)
  • 이은주 (한경대학교 영양조리과학과) ;
  • 이재준 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과) ;
  • 이현주 (한경대학교 영양조리과학과)
  • Received : 2011.09.06
  • Accepted : 2011.12.20
  • Published : 2012.01.31
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Abstract

This study examined the effects of baked garlic powder on the lipid metabolism in rats fed a high-fat/highcholesterol diet for 4 weeks to induce hyperlipidemia. Male Sprague-Dawley rats were assigned to four groups according to the dietary fat, cholesterol and baked garlic powder levels. The experimental groups were normal diet group (N), a high-fat/high-cholesterol diet group (C), a high-fat/high-cholesterol diet with 1.5% baked garlic powder group (GPL) and a high-fat/high-cholesterol diet with 3% baked garlic powder group (GPH). The body weight gain, food intake and food efficiency ratio were similar in the experimental groups. The epididymal adipose tissues weight of the C group was higher than that of the N group, whereas those of the groups fed baked garlic powder were decreased gradually. The ALT and ALP activities were similar in the C groups, but the serum AST and LDH activities elevated by a high-fat/high-cholesterol diet were decreased significantly by feeding a 3% baked garlic powder diet. The serum triglyceride, total cholesterol and LDL-cholesterol levels as well as the atherogenic index and cardiac risk factor tended to decrease in the groups fed baked garlic powder than the C group, whereas the serum HDL-cholesterol level was lower in the C group and remarkably in groups fed baked garlic powder than the control group. The total cholesterol level in the liver and mesenteric adipose tissue and the triglyceride level in epididymal tissue were lower in the groups fed baked garlic powder than the C group. These results suggest that baked garlic powder reduces the serum lipid components and improves the lipid metabolism in hyperlipidemic rats induced with a high-fat/high-cholesterol diet.

본 연구는 구운 마늘 분말의 혈청, 간 및 지방조직의 체내 지질대사 개선효과와 항비만 효과를 살펴보기 위하여 5주령된 흰쥐 수컷 32마리를 10일간 적응시킨 후 정상군(N), 고지방-고콜레스테롤 식이군(대조군, C), 고지방-고콜레스테롤 식이와 1.5% 구운 마늘 분말 첨가군(GPL) 및 고지방-고콜레스테롤 식이와 3% 구운 마늘 분말 첨가군(GPH)으로 나누어 4주간 실시하였다. 체중증가량과 식이섭취량은 실험군들간에 유의한 차이를 보이지 않았고, 식이효율은 고지방-고콜레스테롤을 급여한 군들(C, GPL, GPH)이 대조군(C)에 비하여 증가하였다. 체중 100 g 당 간조직 및 장간막지방조직의 무게는 구운 마늘 급여에 따른 유의차는 없었고, 부고환지방 조직의 무게는 구운 마늘 분말 1.5%와 3% 첨가군들(GPL, GPH)이 대조군(C)에 비하여 유의하게 감소하였다. 고지방-고콜레스테롤 식이로 인하여 혈청 ALT, AST, ALP 및 LDH 활성이 증가되었으나, 혈청 AST 및 LDH 활성은 3% 구운 마늘 분말 첨가로 유의하게 저하되었다. 혈청 중성지방과 총콜레스테롤 함량은 고지방-고콜레스테롤 식이 급여로 유의하게 증가하였으며, 구운 마늘 분말 첨가 수준이 증가할수록 감소하는 경향을 보였으며 특히 3% 구운 마늘 분말 첨가군(GPH)은 대조군(C)에 비하여 유의하게 감소되었다. 고지방-고콜레스테롤 식이 급여로 감소되어진 혈청 HDL-콜레스테롤 함량은 구운 마늘 분말 첨가로 증가하였고, 혈청 LDL-콜레스테롤 함량, 동맥경화지수 및 심혈관위험지수는 구운 마늘 분말 첨가로 감소하여 동맥경화 및 심혈관 질환의 발병 위험률 감소 효과를 나타내었다. 고지방-고콜레스테롤 식이로 간과 지방조직의 중성지방 및 총콜레스테롤 함량이 증가되었으며, 고지방-고콜레스테롤 식이로 증가된 간조직과 장간막지방조직의 총콜레스테롤 함량, 부고환조직의 중성지방 함량은 구운 마늘 급여로 저하되었다. 이상의 실험 결과 구운 마늘은 고지방-고콜레스테롤 식이를 급여한 흰쥐의 혈청, 간 및 지방조직의 지질대사 개선에 긍정적인 영향을 미치는 것으로 나타나 체내 지질대사 개선과 심혈관계 질환 예방효과가 클 것으로 기대된다.

Keywords

References

  1. Geise J. 1994. Spices and seasoning blends: a taste for all seasons. Food Technol 48: 87-98.
  2. Park KW. 1996. Spice vegetable cultivation and use. Korea University press, Seoul, Korea. p 1-8.
  3. Lee TB. 1979. Illustrated flora of Korea. Hangmunsa, Seoul, Korea. p 203.
  4. Hoong MS. 1992. The culture of food in Korea. Kyomoonsa, Seoul, Korea. p 79.
  5. Kim KJ, Do JR, Kim HK. 2005. Antimicrobial, antihypertensive and anticancer activities of garlic extract. Korean J Food Technol 37: 228-232.
  6. Kwon SK. 2003. Organosulfur compounds from Allium sativum and physiological activities. J Appl Pharmacol 11: 8-32.
  7. Lee JM, Cha TY, Kim SH, Kwon TK, Kwon JH, Lee SH. 2007. Monitoring on extraction conditions for physicochemical qualities of ethanol extract from garlic. J Korean Soc Food Sci Nutr 36: 1198-1204. https://doi.org/10.3746/jkfn.2007.36.9.1198
  8. Small LD, Bailey JH, Cavallito CJ. 1949. Comparison of some properties of thiosulfonates and thiosulfinates. J Am Chem Soc 71: 3565-3566. https://doi.org/10.1021/ja01178a531
  9. Lawson LD, Wood SG, Hunges BD. 1991. HPLC analysis of allicin and other thiosulfinates in garlic clove homogenates. Planta Med 57: 263-270. https://doi.org/10.1055/s-2006-960087
  10. Jo KS, Kim HK, Ha JH, Park MH, Shin HS. 1990. Flavor compounds and storage stability of essential oil from garlic distillation. Korean J Food Sci Technol 22: 840-845.
  11. Shin JH, Jung KM, Lee SJ, Yang SM. 2009. Biological activities of dried garlic, red ginseng and their mixture. J Korean Soc Food Sci Nutr 38: 1633-1639. https://doi.org/10.3746/jkfn.2009.38.12.1633
  12. Kim YD, Seo JS, Kim KJ, Kim KM, Hur CK, Cho IK. 2005. Component analysis by different heat treatments of garlic (Allium saivum L.). Korean J Food Preserv 12: 161-165.
  13. Hwang IG, Woo KS, Kim DJ, Hong JT, Hwang BY, Lee YR. 2007. Isolation and identification of an antioxidant substance from heated garlic. Food Sci Biotechnol 16: 963-966.
  14. Chun HJ, Paik JE. 1997. Effect of heart treatment of garlic added diet on the blood of spontaneously hypertention rat. J Korean Soc Food Sci Nutr 26: 103-108.
  15. Kang JA, Kang JS. 1997. Effect of garlic and onion on plasma and liver cholesterol and triglyceride and platelet aggregation in rats fed basal or cholesterol supplemented diets. Kor J Nutr 30: 132-138.
  16. Jo HJ, Choi MJ. 2002. Effect of 1% garlic powder on serum and liver lipid and plasma amino acid concentration in rats fed cholesterol diet. J Korean Soc Food Sci Nutr 31:98-103. https://doi.org/10.3746/jkfn.2002.31.1.098
  17. Sheo HJ. 2000. Toxic effects of the megadose garlic juice in the rats. J Korean Soc Food Sci Nutr 29: 485-492.
  18. Seo HJ. 1999. Effects of garlic on the blood lipids and other serum components in rats. J Korean Soc Food Sci Nutr 28: 1339-1348.
  19. Lee YC. 1991. Hypercholesterolemia in Korea and nutritional factors. Korea Soc Lipidol Atherosc 1: 111-122.
  20. Reeves PG, Nielson FH, Fahey Jr GC. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-1951. https://doi.org/10.1093/jn/123.11.1939
  21. Friedwald W, Levy R, Fredrickson D. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502.
  22. Rosenfeld L. 1989. Lipoprotein analysis. Arch Pathol Lab Med 113: 1101-1110.
  23. Folch J, Lees M, Sloane-Stanley G. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509.
  24. Zlatkis A, Zak B. 1969. Study of a new cholesterol reagent. Anal Biochem 29: 143-148. https://doi.org/10.1016/0003-2697(69)90017-7
  25. Biggs HG, Erikson TM, Moorehead WR. 1975. A manual colorimetric assay of triglyceride in serum. Clin Chem 21: 437-441.
  26. Joo EJ. 1978. A study on the changes of components in some organs and growth rate of Albino rats by feeding of experimental diet supplemented with garlic. MS Thesis. Sookmyung Women's University, Seoul, Korea. p 13-16.
  27. Ahmed RS, Sharma SB. 1997. Biochemical studies on combined effects of garlic (Allium sativum Linn) and ginger (Zingiber officinale Rosc) in albino rats. Indian J Exp Biol 35: 841-843.
  28. Kim RJ, Lee SJ, Kim MJ, Hwang CR, Kang JR, Jung WJ, Sung NJ. 2010. Effects of fresh, red and black garlic powder on lipid metabolism of obese rats induced by high fat diet. J Agric Life Sci 44: 159-170.
  29. Elkayam A, Mirelman D, Peleg E., Wilchek M, Miron T, Rabinkov A, Oron-Herman M, Rosenthal T. 2003. The effects of allicin on weight in frucose-induced hyperinsulinemic, hyperlipidemic, hypertensive rats. Am J Hypertens 16: 1053-1056. https://doi.org/10.1016/j.amjhyper.2003.07.011
  30. Augusti KT, Mathew PT. 1974. Lipid lowering effect on allicin (diallyl disulfide-oxide) on long term feeding to normal rats. Experientia 15: 468-470.
  31. Kang MJ, Lee SJ, Shin JH, Kang SK, Kim JG, Sung NJ. 2008. Effect of garlic with different processing on lipid metabolism in 1% cholesterol fed rats. J Korean Soc Food Sci Nutr 37: 162-169. https://doi.org/10.3746/jkfn.2008.37.2.162
  32. Kwon MJ, Nam TJ. 2006. Effects of mesangi (Capsosiphon fulvecens) powder on lipid metabolism in high cholesterol fed rats. J Korean Soc Food Sci Nutr 35: 530-535. https://doi.org/10.3746/jkfn.2006.35.5.530
  33. Plaa GL, Charbonneau M. 1994. Detection and evaluation of chemically induced liver injury. In Principles and Methods of Toxicology. Hayes AW, ed. Raven Press, New York, NY, Korea. p 839-870.
  34. Lim SS, Kim MH, Lee JH. 1997. Effect of Artemisia princeps var orientalis and Circium japonicum var ussuriense on liver function body lipid and bile acid of hyperlipidemic rat. Korean J Nutr 30: 797-80.
  35. Ki HY, Song SW, Ha CS, Han SS. 1993. Effects of the population density on growth and various physiological values of Sprague-Dawely rats. Korean J Ani Sci 9: 71-82.
  36. Hikino H, Tohkin M, Bukiso Y, Namiki T, Nishimura S, Takeyama K. 1986. Antihepatotoxic action of Allium sativum bulbs. Planta Med 52: 163-168. https://doi.org/10.1055/s-2007-969111
  37. Joseph PK, Rao KR, Sundaresh CS. 1989. Toxic effects of garlic extract and garlic oil in rats. Indian J Exp Biol 27: 977-979.
  38. Yu YY, Shaw MY. 1994. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids 29: 189-193. https://doi.org/10.1007/BF02536728
  39. Gebhardt R. 1991. Inhibition of cholesterol biosynthesis by a water-soluble garlic extract in primary cultures of rat hepatocytes. Arzneimittelforschung 41: 800-804.
  40. Han SY, Hu Y, Anno T, Yanagita T. 2002. S-propyl cysteine reduces the secretion of apolipoprotein B100 and triacylglcerol by HepG2 cells. Nutrition 18: 505-509. https://doi.org/10.1016/S0899-9007(02)00749-9
  41. Kamanna VS, Chandrasekhara N. 1982. Effect of garlic (Allium sativum Linn.) on serum lipoproteins and lipoprotein cholesterol levels in albino rats rendered hypercholesteremic by feeding cholesterol. Lipids 17: 483. https://doi.org/10.1007/BF02535329
  42. Choi MS, Koh ET, Stewart TJ. 1982. Effects of garlic on lipid metabolism in rats fed cholesterol or lard. J Nutr 112: 241-248. https://doi.org/10.1093/jn/112.2.241
  43. Jo HJ, Choi MJ. 2002. Effect of 1% garlic powder on serum and liver lipid and plasma amino acid concentration in rats fed cholesterol diet. J Korean Soc Food Sci Nutr 31: 98-103. https://doi.org/10.3746/jkfn.2002.31.1.098
  44. Lee SJ, Shin JH, Kang MJ, Jung WJ, Ryu JH, Kim RJ, Sung NJ. 2010. Antioxidants activity of aged red garlic. J Life Sci 20: 775-781. https://doi.org/10.5352/JLS.2010.20.5.775

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