Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Comparative Analysis of Endophytic Bacterial Communities in the Roots of Rice Grown under Long-term Fertilization Practice using Pyrosequencing Method

파이로시퀀싱을 이용한 비료 장기 연용지의 벼 뿌리 내생세균의 군집 분석

  • Kim, Byung-Yong (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Jae-Hyung (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Song, Jaekyeong (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Myung-Sook (Soil & Fertilization Division, National Academy of Agricultural Science, RDA) ;
  • Weon, Hang-Yeon (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration)
  • 김병용 (국립농업과학원 농업미생물과) ;
  • 안재형 (국립농업과학원 농업미생물과) ;
  • 송재경 (국립농업과학원 농업미생물과) ;
  • 김명숙 (국립농업과학원 토양비료과) ;
  • 원항연 (국립농업과학원 농업미생물과)
  • Received : 2012.11.14
  • Accepted : 2012.12.07
  • Published : 2012.12.31
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Abstract

Bacterial endophytes may be important factors in plant growth and ecologically relevant functions in rice. Using pyrosequencing technology, we analyzed the composition of endophytic bacterial communities that colonized the roots of rice cultivated in long-term fertilized (APK) and non-fertilized (NF) paddy soils. A total of 1,900 reads were obtained from 2 samples. All sequences were classified into 177 OTUs (APK sample) or 72 OTUs (NF sample) at a 97% similarity cut-off. Twenty-two OTUs were shared between the 2 samples, and these were also the most dominant OTUs in both samples. Proteobacteria was the most dominant phylum with 90.2%, followed by Actinobacteria (7.1%) and Bacteroidetes (1.1%). Furthermore, Pseudomonas was the most abundant genus in both samples. We observed clear differences in the structure of the endophytic bacterial community structure between the 2 samples. Notably, the distributions of Alphaproteobacteria and Gammaproteobacteria were markedly different. The diversity index of the APK sample was higher than that of the NF sample. These findings showed that the endophytic bacterial community of rice roots was affected by the presence of fertilizers in the rice field soil.

화학비료의 장기 시용이 벼 내생 세균 군집에 미치는 영향을 조사하기 위해서 국립농업과학원의 장기 비료 연용 포장에서 재배한 벼 뿌리의 내생균의 군집을 파이로시퀀싱 기법으로 분석하였다. 3요소구 (APK)와 무비구 (NF) 시료에서 직접 DNA를 추출하여 세균에 특이적인 barcode PCR을 수행한 후 454 파이로시퀀싱을 하였다. 두 시료 (3요소구, 무비구)에서 1,900개의 염기서열을 얻었으며, 각각 177개와 72개의 OTU로 분류하였다. 두 시료는 22개의 OTU를 공유하였으며, 이들 OTU는 두 시료에서 모두 우점하였다. 특히 Pseudomonas속에 속하는 OTU의 비율이 매우 높았다. 문 (phylum) 수준에서 우점하는 내생균은 두 시료 모두 Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Actinobacteria 등 이었다. 처리구별로 계산한 다양성 지수는 3요소구 시료에서 더 높았다. 본 연구를 통해 장기간 비료 시용은 식물체내 존재하는 내생균 군집 구조에 영향을 주며, 벼 뿌리의 내생 세균의 군집 다양성을 증가시키는 것을 알 수 있었다.

Keywords

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