한국잠사곤충학회지 (Journal of Sericultural and Entomological Science)

한국잠사학회 (Korean Society of Sericultural Science)
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호박벌의 휴면 제어 관련 유전자 탐색 및 특성 분석

Molecular characterization of diapause-related genes in the bumblebee, Bombus ignitus

  • 황재삼 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 박인균 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤은영 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 정덕웅 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤형주 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이상범 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김성렬 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이준하 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김인우 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 권용남 (농촌진흥청 국립농업과학원 농업생물부)
  • Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Park, In-Gyun (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Jung, Duck-Oung (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yoon, Hyung Joo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, Sang Beom (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, Joon-Ha (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, In-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kwon, Young Nam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
  • 투고 : 2012.10.07
  • 심사 : 20120000
  • 발행 : 2012.12.30
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초록

To find diapause-related genes, we were performed by differential hybridization with three types of [${\alpha}-^{32}P$]dCTP-labeled total cDNA probes synthesized from diapause-prepared, diapause-maintained and diapause-activated stage of Bombus ignitus queen. Nine individual cDNA clones were found to be differentially expressed in diapause-maintained and diapause-activated stage. Among these clones, BIDC9(BIDC ; Bombus ignitus differentially expressed clone) was analyzed through full-length sequencing and expression pattern analysis. This clone was specifically expressed in the thorax organ. The effect of Juvenile hormone analog(JHA) and $CO_2$ treatment was examined. JHA treatment induced the expression of BIDC9 cDNA clone abruptly after 4 day of treatment. $CO_2$ treatment induced also the clone after 2 day of treatment. BIDC9 cDNA was identified as Bombus ignitus diapause gene contained an open reading frame of 1376 bp encoding 255 amino acids.

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참고문헌

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