Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Microscopic Feature, Protein Marker Expression, and Osteoinductivity of Human Demineralized Dentin Matrix

  • Park, Sung-Min (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Hwang, Jung-Kook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Kim, Young-Kyun (Department of Oral and Maxillofacial Surgery, Section of Dentistry, Seoul National University Bundang Hospital) ;
  • Um, In-Woong (R&D Institute of Korea Tooth Bank) ;
  • Lee, Geun-Ho (Department of Neurology, College of Medicine, Dankook University) ;
  • Kim, Kyung-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
  • Received : 2012.11.07
  • Accepted : 2012.12.13
  • Published : 2012.12.30
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Abstract

Purpose: This study examined the scanning electron microscopic feature, protein marker expression and osteoinductive activity of demineralized dentin matrix (DDM) from human for nude mice. Materials and Methods: Twenty healthy nude mice, weighing about 20 g were used for study. DDM from Human was prepared and implanted into the dorsal portion of nude mouse. Before implantation, DDM was examined by scanning electron microscopy (SEM). Nude mice were sacrificed at 2 weeks, 4 weeks and 8 weeks after DDM grafting and evaluated histologically by H-E, MT staining. And also immunohistochemistry analysis (ostecalcin, osteopontin) was performed. Result: Dentinal tubules and collagen fibers were observed by SEM of dentin surface of DDM. The DDM induced bone and cartilage independently in soft tissues. And, the histological findings showed bone forming cells like osteoblasts, fibroblasts at 2, 4 and 8 weeks. On immunohistochemistry analysis, osteocalcin and osteopontin positive bone forming cells were observed. Conclusion: This results showed that the DDM from human has osteoinductive ability and is a good alternative to autogenous bone graft materials.

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References

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