The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Development of replacement diets for improved growth and survival rate of scallop juvenile Patinopecten yessoensis

큰가리비 Patinopecten yessoensis 치패의 성장 및 생존율 향상을 위한 대체 먹이원 개발

  • 남명모 (국립수산과학원 동해수산연구소) ;
  • 박진철 (국립수산과학원 동해수산연구소) ;
  • 박미선 (국립수산과학원 동해수산연구소) ;
  • 이주 (국립수산과학원 동해수산연구소)
  • Received : 2012.06.10
  • Accepted : 2012.06.19
  • Published : 2012.06.30
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Abstract

This study was done to examine the effect of several diets (Phytoplankton = PHY, Shellfish Diet 1800 = INS, Oil type = OTE, Powder type = PTE) on growth, survival rate and biochemical composition of scallop juvenile Patinopecten yessoensis. The highest survival rate were observed in PTE + PHY (90%). The highest shell length and shell height was observed in PHY and PTE + PHY diet (P > 0.05). The growth with PTE and OTE diet was the lowest in shell length and shell height (P < 0.05). On the other hand, the shell width and meat weight were highest in PHY and PTE + PHY, while the lowest in PTE and OTE (P < 0.05). The content of fatty acids such as DHA and n-3 PUFA levels was significantly higher in the juvenile fed on PTE + PHY than in those fed on PHY and INS alone. Also, the total protein ranged 55.5 to 65.2% in PHY + INS, while 44.8%, 47.9% in PTE and OTE respectively. The RNA and DNA contents were the highest in PHY and PTE + PHY, while the lowest in PTE and OTE (P < 0.05). RNA/DNA ratio significantly higher in juvenile with PHY + INS than those with PTE and OTE alone (P < 0.05). The combination of PTE + PHY could improve the growth and survival of scallop juvenile. Our results suggested that PTE could partially replace live algae in bivalve laval rearing.

큰가리비 치패의 대체 먹이원을 개발하기 위해 각기 다른 먹이원을 공급하여 성장, 생존율 및 체내 조성을 조사하였다. 생존율은 PTE + PHY 실험구에서 90.0%로 가장 높게 나타났다. 각장과 각고는 PHY에서 가장 높게 나타났으나 PTE +PHY 실험구와의 유의적인 차이는 없었다. 반면, PTE와 OTE 단독구는 유의적으로 가장 낮게 나타났다. 한편, 각폭 및 전중량도 동일한 경향을 보여 PHY 단독 실험구와 PTE+PHY 혼합 실험구에서 유의적으로 가장 높게 나타난 반면 PTE와 OTE 단독구는 가장 낮은 값을 보였다. 한편, 체내 지방산 분석에서 DHA 함량은 기존 먹이인 미세조류 단독 실험구 (PHY) 와 경제적인 먹이원인 INS 실험구에 비해 지질강화원인 PTE와 OTE가 혼합된 실험구에서 더욱 높은 것으로 나타났으며, 이러한 높은 값에 기인하여 n-3 PUFA도 높았다. 또한, 총 단백질 함량은 주요 단백질원인 PHY와 INS가 들어간 실험구가 55.5-65.2%의 함량을 보인반면 지질영양강화원만 들어간 PTE, OTE 단독 실험구는 각각 44.8%, 47.0%로 가장 낮은 함량을 나타내었다. 필수아미노산 함량도 동일한 경향을 보여 단순 지질강화원 실험구에서는 낮았다. 한편, 체내 핵산 분석에서 RNA 값은 PHY 단독 실험구와 PTE+PHY 혼합구에서 0.76으로 가장 높게 나타났으나 PTE, OTE 단독구는 각각 0.35, 0.32로 가장 낮게 나타났다. DNA 값도 PHY 단독 실험구와 PTE + PHY 혼합구에서 3.95으로 가장 높게 나타난 반면 PTE, OTE 단독구는 유의적으로 가장 낮았다. 이러한 경향에 의해 RNA/DNA ratio 값도 PHY와 INS가 들어간 단독구와 몇몇 혼합구가 PTE와 OTE 단독구 보다 높았다. 이상의 결과를 통해서 미세조류 단독 공급구인 PHY에 PTE를 혼합해 준 PTE+PHY 혼합 공급구는 큰가리비 치패의 성장, 생존율 및 체내 조성을 향상시켜 주는 것으로 나타났다. 본 실험을 통해 새롭게 대체 개발된 PTE+PHY 혼합구는 이매패류 양식에서 차지하는 미세조류 생산비용의 절감 효과를 기대해 볼 수 있는 것으로 경제적인 측면에서 매우 유용한 결과라 할 수 있다.

Keywords

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