Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Functionalities of Squid Liver Hydrolysates

오징어 간 가수분해물의 기능성

  • Lee, Su-Seon (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyengsang National University) ;
  • Park, Si-Hyang (Sun Marine Biotechnology Co.) ;
  • Park, Joo-Dong (Food R&D, CJ Cheiljedang) ;
  • Konno, Kunihiko (Lab of Food Biochemistry, Faculty of Fisheries, Hokkaido University) ;
  • Choi, Yeung Joon (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyengsang National University)
  • 이수선 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 박시향 (선마린바이오테크) ;
  • 박주동 (CJ 제일제당 식품연구소) ;
  • ;
  • 최영준 (경상대학교 해양식품공학과/해양산업연구소)
  • Received : 2012.10.16
  • Accepted : 2012.11.19
  • Published : 2012.12.31
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Abstract

The autolysate and hydrolysate of a common squid liver, Todarodes pacificus, were prepared. Autolysis (liver ratio, pH, temperature) and Protamex-treated hydrolysis (pH, temperature, ratio of protease to liver) conditions were optimized by response surface methodology using central composite design for under 1 hr of hydrolysis time. The desirability profile indicated that maximum DH could be achieved at a squid liver of 93.5%, pH 6.4, and $47^{\circ}C$ in autolysis, while that of Protamex-treated hydrolysis did at a Protamex-to-squid liver level of 0.33%, pH 6.0, and $55^{\circ}C$. Three amino acids, proline, cysteine, and methionine, were not detected in the total amino acid composition of the Protamex-treated hydrolysate, while they were detected in the free amino acid composition. Cadmium was $8.32{\pm}0.03$ mg/100 g-powder for raw, $3.56{\pm}0.02$ mg/100 g-powder for the autolysate, and $13.26{\pm}0.04$ mg/100 g powder for the Protamex-treated hydrolysate. The major molecular weight ranged from 1.0 to 1.5 kDa for the autolysate and from 210 to 470 Da for the Protamex-treated hydrolysate. Food functionalities of the autolysate, such as surface hydrolphobicity, emulsion activity index, emulsion stability, water, and fat adsorption, were similar to the Protamex-treated hydrolysate. Both the autolysate and Protamex-hydrolysate showed high inhibitory activities on the angiotensin-I converting enzyme. Cell toxicity against the HepG2 cell line was not detected in the autolysate or the Protamex-treated hydrolysate by 200 ${\mu}g/mL$.

북해도산 오징어 간의 유효이용을 목적으로 자가소화물과 Protamex 가수분해물을 제조하고 이들의 식품학적 특성과 몇 가지 기능성을 조사하였다. 자가소화를 위한 최적 조건은 간의 함량 93.5%, pH 6.4, 항온온도 $47^{\circ}C$였으며, Protamex 가수분해의 최적 조건은 오징어 간에 대한 Protamex의 첨가비율 0.33%, pH 6.0, 가수분해온도 $55^{\circ}C$이었다. Promatex 가수분해물의 총 아미노산 조성에서 proline, cysteine 및 methionine이 검출되지 않았으나, 유리아미노산 조성에서는 검출되었다. 오징어 간, 자가소화물 및 Promatex 가수분해물의 카드늄 함량은 건조 중량으로 각각 $8.32{\pm}0.03$ mg/100 g, $3.56{\pm}0.02$ mg/100 g, $13.26{\pm}0.04$ mg/100 g으로서 식품안전 규정 이하의 농도로 감소시키기 위한 별도의 공정이 필요하다. 자가소화물의 주요 저분자 물질의 분자량은 1.0~1.5 kDa인 반면, Protamex 가수분해물은 210~470 Da이었다. 자가소화물과 Protamex 가수분해물 간에 표면 소수성, 유화활성 지표, 유화안정성, 지방 및 수분 흡착능은 큰 차이를 보이지 않았으며, 높은 ACE 효소 저해효과가 관측되었다. 한편 HepG2 세포주에 대한 독성은 200 ${\mu}g/mL$까지 관측되지 않았다.

Keywords

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