Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Screening for Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 Using Molecular Marker in Tomato Germplasm

분자마커를 이용한 토마토 시들음병 race 3 저항성 토마토 유전자원 탐색

  • Hur, On Sook (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ro, Na Young (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ko, Ho Cheol (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Sang Gyu (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Rhee, Ju Hee (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Gwag, Jae Gyun (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Oh, Se Jong (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration)
  • 허온숙 (국립농업과학원 농업유전자원센터) ;
  • 노나영 (국립농업과학원 농업유전자원센터) ;
  • 고호철 (국립농업과학원 농업유전자원센터) ;
  • 김상규 (국립농업과학원 농업유전자원센터) ;
  • 이주희 (국립농업과학원 농업유전자원센터) ;
  • 곽재균 (국립농업과학원 농업유전자원센터) ;
  • 오세종 (국립농업과학원 농업유전자원센터)
  • Received : 2012.10.05
  • Accepted : 2012.12.14
  • Published : 2012.12.31
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Abstract

Fusarium wilt, caused by three races of Fusarium oxysporum f. sp. lycopersici, is one of the most important disease of tomato (Solanum lycopersicum) worldwide. A total of 1,906 tomato accessions were screened for the resistance to Fusarium wilt using I-3 SNP marker and high resolution melting analysis. Results showed that 97 accessions were homozygous resistant, 8 accessions were heterozygous resistant and 1,801 were homozygous susceptible. Accessions containing resistance were identified in 65 accessions of S. lycopersicum var. lycopersicum, 13 accessions of S. lycopericum var. cerasiform, 8 accessions of S. pimpinellifolium, 3 accessions of S. habrochaites, 3 accessions of S. corneliomulleri, 1 accession of S. galapagense, 3 accessions of S. peruvianum, 1 accession of S. chilense. For accurate evaluation of the Fusarium wilt resistance, however, screening to race 1 and race 2 and bio-assay still remain to be evaluated.

세 개의 race가 보고되어 있는 Fusarium oxysporum f. sp. lycopersici는 전 세계적으로 그 피해가 막대한 토마토 시들음병의 원인균으로 알려져 있다. 토마토 유전자원 1,906 자원을 대상으로 토마토 시들음병 race 3 저항성 유전자 I-3의 유전형을 high resolution melting 법을 사용하여 분석하였다. 총 97점의 자원에서 homozygous resistant 피크가 확인되었으며, 8점은 이형접합성, 1,801점은 감수성으로 나타났다. 저항성 자원 97점의 종 분포는 S. lycopersicum var. lycopersicum가 65점, S. lycopersicum var. cerasiforme가 13점, 12종의 야생근연종 중에서 저항성 자원은 S. pimpinellifolium 8점, S. habrochaites 3점, S. corneliomulleri 3점, S. galapagense 1점, S. peruvianum 3점, S. chilense 1점으로 총 19점이었다. 하지만 좀 더 정확한 시들음병 저항성 평가를 위하여 추후에 race 1과 2에 대한 유전분석과 생물검정이 필요하다.

Keywords

References

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