Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Evaluation of Resistance in Pepper Germplasm to Cucumber mosaic virus by High Resolution Melting Analysis

High Resolution Melting Analysis을 이용한 고추 유전자원의 Cucumber mosaic virus 저항성 평가

  • Ro, Na Young (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hur, On Sook (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ko, Ho Cheol (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Sang Gyu (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Rhee, Ju Hee (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Gwag, Jae-Gyun (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, Jin-Kyung (Department of Plant Science, and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kang, Byoung-Cheorl (Department of Plant Science, and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University)
  • 노나영 (국립농업과학원 농업유전자원센터) ;
  • 허온숙 (국립농업과학원 농업유전자원센터) ;
  • 고호철 (국립농업과학원 농업유전자원센터) ;
  • 김상규 (국립농업과학원 농업유전자원센터) ;
  • 이주희 (국립농업과학원 농업유전자원센터) ;
  • 곽재균 (국립농업과학원 농업유전자원센터) ;
  • 권진경 (서울대학교 식물생산과학부) ;
  • 강병철 (서울대학교 식물생산과학부)
  • Received : 2012.09.29
  • Accepted : 2012.12.05
  • Published : 2012.12.31
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Abstract

In this study, total number of 1941 Capsicum accessions conserved at RDA Genebank was evaluated for their response to Cucumber mosaic virus (CMV). These accessions were composed with 9 species originated from 89 countries, included 839 Capsicum annuum, 277 C. baccatum, 395 C. chinense, 343 C. frutescens, 49 C. pubescens, and other 38 wild pepper species (C. chacoense, C. galapagoense, etc.). Resistant to CMV was screened with the 240H02SP6 SNP marker related to the Cmr1 (Cucumber mosaic resistance 1). Eighty nine accessions of pepper germplasm were resistant to CMV based on the marker. One hundred sixty two accessions showed heterozygosity. One thousand two hundred seventy accessions were susceptible to CMV. Four hundred twenty accessions did not show distinction by 240H02SP6 marker. These 89 resistant pepper germplasm can be used in a pepper breeding program against CMV.

농업유전자원센터에 보존하고 있는 고추 유전자원 1,941점을 240H02sp6 SNP 마커를 이용하여 Cucumber mosaic virus 저항성 정도를 평가하였다. 1941점의 고추 유전자원에서 89점이 저항성, 162점이 이형접합성, 1270점이 이병성으로 분석되었다. 고추 종별로는 C. annuum은 총 839자원 중 38점이 저항성으로 나타났으며, C. baccatum은 277점 중 23%에 해당하는 자원만 분석이 되었고 분석된 자원 중 20점이 저항성을 나타났다. C. chinense는 21점이 저항성, C. frutescens는 8점이 저항성을 보였다. C. pubescens는 K157779 자원만 이병성을 나타내었으며, Capsicum sp. 자원들 중에는 저항성 자원이 없었다. 야생근연종인 C. chacoense는 15점이 분석되어 1점이 저항성, 4점이 이형접합성, 6점이 이병성을 보였고, C. galapagoense, C. tovarii는 각 한 점씩 분석되었고 모두 이병성으로 나타났다.

Keywords

References

  1. Abdalla, O. A., Desjardine, P. R. and Dodds, J. A. 1991. Identification, disease incidence, and distribution of viruses infecting peppers in California. Plant Dis. 75: 1019-1023. https://doi.org/10.1094/PD-75-1019
  2. Benner, C. P., Kuhn, C. W., Demski, J. W., Dobson, J. W., Colditz, P. and Nutter, F. W. Jr. 1985. Identification and incidence of pepper viruses in northeastern Georgia. Plant Dis. 69: 999-1001.
  3. Boccardo, G. and Conti, M. 1973. Purification and properties of nasturtium ringspot virus. J. Phytopathol. 78: 14-24. https://doi.org/10.1111/j.1439-0434.1973.tb04146.x
  4. Cai, W. Q., Fang, R. X., Shang, H. S., Wang, X., Zhang, F. L., Li, Y. R., Zhang, J. C., Cheng, X. Y., Wang, G. L. and Mang, K. Q. 2003. Development of CMV- and TMV-resistant transgenic chili pepper: Field performance and biosafety assessment. Mol. Breeding 11: 25-35. https://doi.org/10.1023/A:1022655204552
  5. Chagne, D., Gasic, K., Crowhurst, R. N., Han, Y., Bassett, H. C., Bowatte, D. R., Lawrence, T. J., Rikkerink, E. H., Gardiner, S. E. and Korban, S. S. 2008. Development of a set of SNP markers present in expressed genes of the apple. Genomics 92: 353-358. https://doi.org/10.1016/j.ygeno.2008.07.008
  6. Cho, D. W., Oh, J. P., Park, K. W. and Chung, K. H. 2009. Analysis of agricultural characteristics for the virus resistant GM pepper plants grown in three different regions in Korea. Korean J. Hort. Sci. Technol. 27: 521-529.
  7. Cho, J. D., Lee, S. H., Kim, J. S., Choi, K. S. and Chung, B. N. 2007. Study of occurrence of virus disease on the pepper in Korea. BioWave 9: 1-8. (In Korean)
  8. Croxford, A. E., Rogers, T., Caligari, P. D. S. and Wilkinson, M. J. 2008. High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar. New Phytol. 180: 594-607. https://doi.org/10.1111/j.1469-8137.2008.02588.x
  9. Florini, D. A. and Zitter, T. A. 1987. Cucumber mosaic virus (CMV) in pepper (Capsicum annuum L.) in New York and associated yield losses. Phytopathology 77: 652. (Abstract)
  10. Gracia, O. and Gutierrez, L. S. 1982. Broad bean wilt virus in pepper crops in Argentina. Phytopath. Medit. 21: 107-109.
  11. Hofinger, B. J., Jing, H. C., Hammond-Kosack, K. E. and Kanyuka, K. 2009. High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley. Theor. Appl. Genet. 119: 851-865. https://doi.org/10.1007/s00122-009-1094-2
  12. Imoto, M. 1975. Studies on mosaic disease of sweet pepper (Capsicum frutescens L.) 2. Occurrence of mosaic disease of sweet pepper and kinds of its causal viruses in Hiroshima prefecture. Bull. Hiroshima Agr. Expt. Station, Japan 36: 56-66.
  13. Jeong, H. J., Kwon, J. K., Pandeya, D., Hwang, J. N., Hoang, N. H., Bae, J. H. and Kang, B. C. 2012. A survey of natural and ethyl methane sulfonate-induced variations of eIF4E using high-resolution melting analysis in Capsicum. Mol. Breeding 29: 349-360. https://doi.org/10.1007/s11032-011-9550-5
  14. Joshi, R. D. and Dubey, L. N. 1973. Assessment of losses due to CMV on chili. Science and Culture 39: 521-522.
  15. Kang, W. H., Hoang, N. H., Yang, H. B., Kwon, J. K., Jo, S. H., Seo, J. K., Kim, K. H., Choi, D. and Kang, B. C. 2010. Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.). Theor. Appl. Genet. 120: 1587-1596. https://doi.org/10.1007/s00122-010-1278-9
  16. Kim, D. H., Cho, J. D., Kim, J. H., Kim, J. S. and Cho, E. K. 2005. Ultrastructural characteristics of necrosis and stunt disease in red pepper by the mixed infections of Tobacco mosaic virus-U1 and Pepper mild mottle virus and Pepper mottle virus. Plant Pathology J. 21: 252-257. https://doi.org/10.5423/PPJ.2005.21.3.252
  17. Kim, S. H., Yoon, J. B., Do, J. W. and Park, H. G. 2008. A major recessive gene associated with anthracnose resistance to Colletotrichum capsici in chili pepper (Capsicum annuum L.). Breed. Sci. 58: 137-141. https://doi.org/10.1270/jsbbs.58.137
  18. Laird, E. F., Desjardins, P. R. and Dickson, R. C. 1964. Tobacco etch virus and potato virus Y from pepper in southern California. Plant Dis. Rep. 48: 772-776.
  19. Lee, S. H., Lee, J. B., Kim, S. M., Choi, H. S., Park, J. W., Lee, J. S., Lee, K. W. and Moon, J. S. 2004. The incidence and distribution of viral diseases in pepper by cultivation types. Res. Plant Dis. 10: 231-240. (In Korean) https://doi.org/10.5423/RPD.2004.10.4.231
  20. Lee, Y. H., Jung, M., Shin, S. H., Lee, J. H., Choi, S. H., Her, N. H., Lee, J. H., Ryu, K. H., Paek, K. Y. and Harn, C. H. 2009. Transgenic peppers that are highly tolerant to a new CMV pathotype. Plant Cell Rep. 28: 223-232. https://doi.org/10.1007/s00299-008-0637-3
  21. Lehmensiek, A., Sutherland, M. W. and McNamara, R. B. 2008. The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley. Theor. Appl. Genet. 117: 721-728. https://doi.org/10.1007/s00122-008-0813-4
  22. Makkouk, K. M. and Gumpf, D. J. 1976. Characterization of potato virus Y strains isolated from pepper. Phytopathology 66: 576-581. https://doi.org/10.1094/Phyto-66-576
  23. Pae, D. H., Chae, Y., Wang, T. C., Engle, L. M. and Shanmugasundaram, S. 2005. Selection of new breeding materials with resistance to anthracnose in Capsicum annuum. J. Korean Soc. Hort. Sci. 46: 10-12.
  24. Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41: 281-341. https://doi.org/10.1016/S0065-3527(08)60039-1
  25. Park, S. W., An, S. J., Yang, H. B., Kwon, J. K. and Kang, B. C. 2009. Optimization of high resolution melting analysis and discovery of single nucleotide polymorphism in Capsicum. Hort. Environ. Biotechnol. 50: 31-39.
  26. Purcifull, D. E., Zitter, T. A. and Hiebert, E. 1975. Morphology, host range and serological relationships of pepper mottle virus. Phytopathology 65: 559-562. https://doi.org/10.1094/Phyto-65-559
  27. Wittwer, C. T., Reed, G. H., Gundry, C. N., Vandersteen, J. G. and Pryor, R. J. 2003. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin. Chem. 49: 853-860. https://doi.org/10.1373/49.6.853
  28. Yankulova, M. and Kaitazova, P. 1979. Broad bean wilt virus-a new virus on pepper in Bulgaria. Grradinarska I Lozarska Nanka. 16: 48-57.
  29. Yoon, J. B., Do, J. W., Yang, D. C. and Park, H. G. 2004. Interspecific cross compatibility among five domesticated species of Capsicum genus. J. Korean Soc. Hort. Sci. 45: 324- 329.

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