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Effects of Cucurbita moschata, Adlay Seed, and Cudrania tricuspidata Leaf Mixed-powder Diet Supplements on the Visceral Fat, Fecal Amount, and Serum Lipid Levels of the Rats on a High-Fat Diet

늙은 호박, 율무, 꾸지뽕잎 혼합조성물이 고지방식이를 급여한 흰쥐의 내장지방, 배변량 및 혈청지질농도에 미치는 영향

  • Received : 2012.11.07
  • Accepted : 2012.12.07
  • Published : 2012.12.31

Abstract

This study investigated the effects of the diet with Cucurbita moschata (CM), adlay seed (AS), and Cudrania tricuspidata leaf (CTL) mixed-powder on the visceral fat, fecal amount, and serum lipid profiles in rats fed a high-fat diet (HFD). The mixed ratio of CM, AS, and CTL as a functional food ingredient was 1:1:1 on a weight basis. Male Sprague-Dawley rats were fed a high-fat diet (D12492; 45 kcal fat) for 5 weeks during the first phase. In the second phase, which lasted 5 weeks, the rats were divided into six experimental groups. The groups were: a high-fat diet group as a control during the 10 weeks of experimental period; and a high-fat diet with 5, 10, 15, 20, and 30% mixed-powder supplement group. The adipose tissue (RFP, EFP) weights were significantly decreased in the 20% and 30% mixed-powder supplement groups than in the HFD group (p<0.05). The fecal weight produced by the 15%, 20%, and 30% mixed-powder supplement groups was significantly increased during the second phase compared to the first phase (p<0.05). In terms of serum lipids, TG (triglyceride) content was decreased in the 10% and 15% mixed-powder supplement groups. The blood glucose levels were significantly decreased in all experimental diet supplement groups than in the HFD group (p<0.05). The findings show that the high supplement groups could improve the visceral fat accumulation, fecal amount, and blood glucose content compared to the HFD group.

고지방식이(D12492)를 5주 동안 흰쥐에게 급여한 다음 고지방식이에 늙은 호박(Cucurbita moschata), 율무(adlay seed), 꾸지뽕(Cudrania tricuspidata) 분말조성물(1:1:1) 첨가식이를 5주 동안 급여한 결과는 다음과 같다. 실험기간동안 증체량은 혼합조성물 10% 첨가군(B)을 제외하고, 혼합조성물의 첨가 수준이 높을수록 감소 경향을 나타내었다. 식이섭취량은 차이가 없이 혼합조성물의 첨가 수준이 높을수록 다소 증가한 반면, 식이효율은 혼합조성물의 첨가 수준이 높을수록 다소 감소하였다. 내장지방 중 신장주변지방패드(RFP) 무게는 고지방식이대조군(F)보다 혼합조성물 30% 첨가군(E)이 통계적으로 낮았고, 정소상체지방패드(EPF) 무게는 F군보다 혼합조성물 20% 첨가군(D)이 유의하게 낮았다(p<0.05). 배변량의 경우, 실험 전기간(10주) 고지방식이대조군(F)은 실험1기와 실험2기의 배변량이 차이가 없었고, 혼합조성물의 경우 첨가 수준이 높을수록 F군보다 통계적으로 유의하게 증가하였다(p<0.05). 소장길이는 실험군 간에 유의한 차이가 없이 혼합조성물 10%, 15% 및 20% 첨가군(B, C, D)이 F군보다 다소 긴 것으로 나타났고, 대장길이는 F군과 20% 첨가군(D) 간에 통계적으로 유의한 차이가 있었다(p<0.05). 혈청지질중 TC, HDL- 및 LDL-콜레스테롤 농도는 유의적인 차이가 없었고, TG 농도는 혼합조성물 10%(B)와 15% 첨가군(C)이 낮았고, F군과 다른 첨가군은 높았다. 혈당농도는 혼합조성물첨가군이 F군보다 통계적으로 유의하게 낮았다(p<0.05). 혈구세포(CBC)중 RBC와 WBC는 혼합조성물 30% 첨가군(E)이 F군보다 통계적으로 높았고, Hct와 Hb 함량은 E군이 F군보다 높았으며, 혈소판(Platelet) 수는 혼합조성물첨가군(A~E)이 F군보다 통계적으로 유의하게 높았다(p<0.05). 이상에서 고지방식이를 급여한 흰쥐에게 늙은 호박, 율무, 꾸지뽕잎 분말조성물첨가식이를 급여했을 때 체지방량 저하, 배변량 증가, 혈청중 TG 감소 및 혈당치의 유의한 감소작용이 있는 것으로 나타나서, 앞으로 이들 혼합조성물의 적절한 처방으로 체지방 억제, 배변활성 및 지질개선을 위한 소재화 기술개발이 필요한 것으로 판단된다.

Keywords

References

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