Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Syringe Infusion-based Contactless Atmospheric Pressure Ionization Mass Spectrometry for Small and Large Biomolecules

  • Lo, Ta-Ju (Department of Applied Chemistry, National Chiao Tung University) ;
  • Chang, Chia-Hsien (Department of Applied Chemistry, National Chiao Tung University) ;
  • Chen, Yu-Chie (Department of Applied Chemistry, National Chiao Tung University)
  • Received : 2012.12.05
  • Accepted : 2012.12.18
  • Published : 2012.12.31
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Abstract

In this study, we explored a new approach for generating ions of organics and biomolecules using contactless atmospheric pressure ionization (C-API). That is, a tapered capillary (~20 cm) was connected to a syringe, which was coupled to a syringe pump for providing a given flow rate to introduce sample solution to the proximity of a mass spectrometer. The gas phase ions derived from analytes were readily formed in the capillary outlet, which was very close to the mass spectrometer (~1 mm). No external electric connection was applied on the capillary emitter. This setup is very simple, but it can function as an ion source. This approach can be readily used for the analysis of small molecules such as amino acids and large molecules such as peptides and proteins. The limit of the detection of this approach was estimated to be ~10 pM when using bradykinin as the sample. Thus, we believe that this approach should be very useful for being used as an alternative ion source because of its low cost, high sensitivity, simplicity, and ease of operation.

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References

  1. Fenn, J. B.; Mann, M.; Meng, C.; Wang, S. F.; Whitehouse, C. M. Science 1989, 246, 61.
  2. Emmett, M. R.; Andren, P. E.; Caprioli, R. M. J. Neurosci. Methods 1995, 62, 141. https://doi.org/10.1016/0165-0270(95)00070-4
  3. Wilm, M.; Mann, M. Anal. Chem. 1996, 68, 1.
  4. Takats, Z.; Wiseman, J. M.; Gologan, B.; Cooks, R. G. Science 2004, 306, 471. https://doi.org/10.1126/science.1104404
  5. Takats, Z.; Wiseman, J. M.; Cooks, R. G. J. Mass Spectrom. 2005, 40, 1261. https://doi.org/10.1002/jms.922
  6. Miao, Z.; Chen, H.; Liu, P.; Liu, Y. Anal. Chem. 2011, 83, 3994. https://doi.org/10.1021/ac200842e
  7. Laskin, J.; Heath, B. S.; Roach, P. J.; Cazares, L. Anal. Chem. 2012, 84, 141. https://doi.org/10.1021/ac2021322
  8. Zhu, L.; Gamez, C.; Chen, H. W.; Huang, H. X.; Chingin, K.; Zenobi, R. Rapid Commun. Mass Spectrom. 2008, 22, 2993. https://doi.org/10.1002/rcm.3700
  9. McCullough, B. J.; Bristow, T.; O'Connor, G.; Hopley, C. Rapid Commun. Mass Spectrom. 2011, 25, 1445. https://doi.org/10.1002/rcm.5016
  10. Cheng, C-.Y.; Yuan, C.-H.; Cheng, S.-C.; Huang, M.-Z.; Chang, H.-C.; Cheng, T.-L.; Yeh, C.-S.; Shiea, J. Anal. Chem. 2008, 80, 7699. https://doi.org/10.1021/ac800952e
  11. Harper, J. D.; Charipar, N. A.; Mulligan, C. C.; Zhang, X.; Cooks, R.G.; Ouyang, Z. Anal. Chem. 2008, 80, 9097. https://doi.org/10.1021/ac801641a
  12. Santos, V. G.; Regiani, T.; Dias, F. F. G.; Romao, W.; Jara, J. L. P.; Klitzke, C. F.; Coelho, F.; Eberlin, M. N. Anal. Chem. 2011, 83, 1375. https://doi.org/10.1021/ac102765z
  13. Chen, T.-Y; Chao, C.-S; Mong, K.-K. T.; Chen, Y.-C. Chem. Commun. 2010, 46, 8347. https://doi.org/10.1039/c0cc02629h
  14. Chen, T.-Y.; Lin, J.-Y.; Chen, Y.-C. J. Am. Soc. Mass Spectrom. 2010, 21, 1547. https://doi.org/10.1016/j.jasms.2010.04.021
  15. Lo, T.-J.; Chen, T.-Y.; Chen, Y.-C. J. Mass Spectrom. 2012, 47, 480. https://doi.org/10.1002/jms.2043
  16. Hirabayashi, A.; Sakairi, M.; Koizumi, H. Anal. Chem. 1995, 67, 2878. https://doi.org/10.1021/ac00113a023
  17. Dams, R.; Benijts, T.; Gunther, W.; Lambert, W.; De Leenheer, A. Anal. Chem. 2002, 74, 3206. https://doi.org/10.1021/ac0112824
  18. Haddad, R.; Milagre, H. M.; Catharino, R. R.; Eberlin, M. N. Anal. Chem. 2008, 80, 2744. https://doi.org/10.1021/ac702216q
  19. Cody, R. B.; Laramee, J. A.; Durst, H. D. Anal. Chem. 2005, 77, 2297. https://doi.org/10.1021/ac050162j
  20. Sampson, J. S.; Hawkridge, A. M.; Muddiman, D. C. J. Am. Soc. Mass Spectrom. 2006, 17, 1712. https://doi.org/10.1016/j.jasms.2006.08.003
  21. Nemes, P.; Vertes, A. Anal. Chem. 2007, 79, 8098. https://doi.org/10.1021/ac071181r
  22. Haapala, M.; Pól, J.; Saarela, V.; Arvola, V.; Kotiaho, T.; Ketola, R. A.; Franssila, S.; Kauppila, T. J.; Kostiainen, R. Anal. Chem. 2007, 79, 7867. https://doi.org/10.1021/ac071152g
  23. Trimpin, S.; Inutan, E. D.; Herath, T. N.; McEwen, C. N. Mol. Cell Proteom. 2009, 9, 362.
  24. Weston, D. J. Analyst 2010, 135, 661. https://doi.org/10.1039/b925579f
  25. Huang, M.-Z.; Yuan, C.-H.; Cheng, S.-C.; Cho, Y.-T.; Shiea, J. Annu. Rev. Anal. Chem. 2010, 3, 43. https://doi.org/10.1146/annurev.anchem.111808.073702
  26. Alberici, R. M.; Simas, R. C.; Sanvido, G. B.; Romao, W.; Lalli, P. M.; Benassi, M.; Cunha, I. B.; Eberlin, M. N. Anal. Bioanal. Chem. 2010, 398, 265. https://doi.org/10.1007/s00216-010-3808-3
  27. Xiaoxiao, M.; Sichun, Z.; Xinrong, Z. TRAC-Trend Anal. Chem. 2012, 35, 50. https://doi.org/10.1016/j.trac.2011.12.004
  28. McEwen, C. N.; McKay, R. G.; Larsen, B. S. Anal. Chem. 2005, 77, 7826. https://doi.org/10.1021/ac051470k
  29. McEwen, C. N.; Gutteridge, S. J. Am. Soc. Mass Spectrom. 2007, 18, 1274. https://doi.org/10.1016/j.jasms.2007.03.032
  30. Hsieh, C.-H.; Chang, C.-H.; Urban, P. L.; Chen, Y.-C. Anal. Chem. 2011, 83, 2866. https://doi.org/10.1021/ac200479s
  31. Hsieh, C.-H.; Chao, C.-S.; Mong, K.-K. T.; Chen, Y.-C. J. Mass Spectrom. 2012, 47, 586. https://doi.org/10.1002/jms.2983

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