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Distribution of MIC value of antibiotics against Edwardsiella tarda isolated from olive flounder (Paralichthys olivaceus)

양식 넙치 (Paralichthys olivaceus) 에서 분리된 Edwardsiella tarda에 대한 항균제 MIC 값 분포

  • Kim, Myoung Sug (Pathology Division, National Fisheries Research and Development Institute) ;
  • Cho, Ji Young (Deptartment of Marine biotechnology, Soonchunhyang University) ;
  • Seo, Jung Soo (Pathology Division, National Fisheries Research and Development Institute) ;
  • Jung, Sung Hee (Pathology Division, National Fisheries Research and Development Institute) ;
  • Choi, Hye Sung (Pathology Division, National Fisheries Research and Development Institute) ;
  • Park, Myoung Ae (Pathology Division, National Fisheries Research and Development Institute)
  • 김명석 (국립수산과학원 병리연구과) ;
  • 조지영 (순천향대학교 해양생명공학과) ;
  • 서정수 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 최혜승 (국립수산과학원 병리연구과) ;
  • 박명애 (국립수산과학원 병리연구과)
  • Received : 2012.11.05
  • Accepted : 2012.11.27
  • Published : 2012.12.31

Abstract

The minimum inhibitory concentration (MIC) of eight antibiotics against Edwardsiella tarda 49 strains isolated from olive flounder (Paralichthys olivaceus) was determined by the broth microdilution method. E. tarda showed 38.8% and 61.2% resistance to ampicillin and amoxicillin. Both resistance rates of E. tarda were 4.1% against ciprofloxacin and norfloxacin. The $MIC_{50}$ values of oxytetracycline, amoxicillin and oxolinic acid were $64{\mu}g/ml$, $32{\mu}g/ml$ and $4{\mu}g/ml$. The $MIC_{50}$ value to ciprofloxacin was $0.25{\mu}g/ml$ which was lowest among eight antibiotics tested.

넙치에서 분리된 Edwardsiella tarda 49균주에 대한 8종류 항균제의 최소 억제 농도를 broth dilution 법으로 조사하였다. E. tarda는 ampicillin과 amoxicillin에 대해 각각 38.8%와 61.2%의 내성률을 가지고 있었고 ciprofloxacin과 norfloxacin에 대해 4.1%의 내성률을 나타내었다. E. tarda에 대한 oxytetracycline의 $MIC_{50}$ 값은 $64{\mu}g/ml$ 이었고 amoxicillin의 $MIC_{50}$$32{\mu}g/ml$, oxolinic acid의 $MIC_{50}$ 값은 $8{\mu}g/ml$이었다. Ciprofloxacin의 $MIC_{50}$ 값은 $0.25{\mu}g/ml$로 조사한 8종류 항균제 중에서 가장 낮았다.

Keywords

References

  1. Aoki, T. and Kitao, T.: Drug resistant and transferble R plamid in Edwardsiella tarda in fish culture ponds. Fish. Pathol., 15:277-281, 1981. https://doi.org/10.3147/jsfp.15.277
  2. Blaser, J., Stone, B.B., Groner, M. and Zinner, S.H.: Comparative study with enoxacin and netilmicin in a pharmacodynamic model to determine importance of ratio of antibiotic peak concentration to MIC for bactericidal activity and emergence of resistance. Antimicrobial agent and chemotherapy, 31(7):1054-1060, 1987. https://doi.org/10.1128/AAC.31.7.1054
  3. CLSI: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved standard-Seventh edition, Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, USA. 2006a.
  4. CLSI: Performance standards for antimicrobial susceptibility testing; Sixteenth informational supplement M100- S16. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, USA. 2006b.
  5. Hamilton-Miller, J.M.T.: Calculating $MIC_{50}$. Journal of Antimicrobial Chemotherapy, 27:863-875, 1991. https://doi.org/10.1093/jac/27.6.863
  6. Kashuba, A.D.M., Nafziger A.N., Drusano G.L. and Bertino J.S.: Optimising aminoglycoside therapy for nosocomial pneumonia caused by gram-negative bacteria. Antimicrob. Agents Chemother. 43: 623-629, 1999.
  7. Kerr, J.R.: Antibiotic treatment and susceptibility testing. Microbiology. 58: 786-787, 2005.
  8. Kim, E-h. and Aoki, T.: Drug resistance and broad geographical distribution of identical R plasmid of Pasteurella piscida isolated from cultured yellowtail in Japan. Microbiol. Immunol., 37: 103-109, 1993. https://doi.org/10.1111/j.1348-0421.1993.tb03186.x
  9. Kubota, S.S., Kaige, N., Miyazaki, T. and Miyashita, T.: Histopathological studies on edwardsiellosis of tilapia-1. Natural infection. Bulletin of the Faculty of Fisheries, Mie University 9:155-165, 1981.
  10. MacGowan, A.P.: Role of pharmacokinetics and pharmacodynamics: Does the dose matter? Clinical Infectious Diseases 33:238-239, 2001. https://doi.org/10.1086/321855
  11. McKinnon, P.S. and Davis, S.L.: Pharmacokinetic and pharmacodynamic issues in the treatment of bacterial infection diseases. Eur. J. Cln. Microbiol. Infect. Dis. 23:271-288, 2004. https://doi.org/10.1007/s10096-004-1107-7
  12. Nakatsugawa, T.: Edwardsiella tarda isolated from cultured young flounder. Fish Pathology, 18:99-101, 1983. https://doi.org/10.3147/jsfp.18.99
  13. Sae-Oui, D., Muroga, K. and Nakai, T.: A case of Edwardsiella tarda infection in cultured colored carp Cyprinus carpio. Fish Pathology, 19:197-199, 1984. https://doi.org/10.3147/jsfp.19.197
  14. Stock, I. and Wiedemann, B.: Natural antibiotic susceptibilities of Edwardsiella tarda, E. ictaluri, and E. hoshinae. Antimicrobial agents and chemisterapy, 45(8):2245-2255. 2001. https://doi.org/10.1128/AAC.45.8.2245-2255.2001
  15. Thomas, J.K., Forrest, A., Bharnai, S.M., Hyatt, J.M., Cheng, A., Ballow, C. H. and Schentag, J.J.: Pharmacodynamic evaluation of factors associated with the development of bacterial resistance in acutely ill patients during therapy. Antimicrob. agents chemother. 42:521-527, 1998.
  16. Turnidge, J. and Paterson, D.I.: Setting and revising antibacterial susceptibility breakpoints. Clin. Microbiol. Rev., 20:391-408, 2007. https://doi.org/10.1128/CMR.00047-06
  17. Wakabayashi, H. and Egusa, S.: Edwardsiella tarda (Paracolobacterum anguillimoriferum) associated with pond-cultured eel diseases. Bulletin of the Japanese Society of Scientific Fisheries, 39: 931-936, 1973. https://doi.org/10.2331/suisan.39.931
  18. 국립수산과학원: 2011 수산용 의약품 제품요약 해설집. p151. 한국, 보성인쇄. 2011.
  19. 김명석, 김경덕, 김강웅, 박명애, 김진우: 넙치 (Paralichthys olivaceus) 에 투여된 Edwardsiella tarda와 Streptococcus iniae에 대한 혼합백신의 예방효과와 성장에 미치는 영향. 한국어병학회지, 22(3):327-334, 2009.
  20. 김명석, 박성우, 허민도, 정현도: 수온과 세균 감염이 나일틸라피아 (Oreochromis niloticus) 의 oxolinic acid 흡수와 배설에 미치는 영향. 한국어병학회지, 31:677-684, 1998.
  21. 김은희: 넙치에서 분리된 Edwardsiella tarda의 약제내성 전달성 R plasmid. 한국어병학회지, 12(2): 115-121, 1999.
  22. 정승희, 최동림, 김진우, 조미라, 서정수, 지보영: Oxytetracycline을 근육 주사한 넙치 (Paralichthys olivaceus) 의 약물동태학적 특성. 한국어병학회지, 22(1):91-95, 2009a.
  23. 정승희, 최동림, 김진우, 조미라, 서정수, 지보영: Oxytetracycline의 약욕 및 경구투여에 따른 넙치 (Paralichthys olivaceus) 체내 약물동태학적 특 성. 한국어병학회지, 21(2):107-117, 2008.
  24. 정승희, 최동림, 김진우, 조미라, 지보영, 서정수: Oxolinic acid의 경구투여, 주사 및 약욕에 따른 넙치, Paralichthys olivaceus 체내 약물동태학적 특성. 한국어병학회지, 22(2):125-135, 2009b.
  25. 조미영, 김명석, 권문경, 지보영, 최혜승, 최동림, 박경현, 이창훈, 김진도, 이주석, 오윤경, 이덕찬, 박신후, 박명애: 2005년부터 2006년 사이 우리나라 양식 넙치, Paralichthys olivaceus의 세균성 질병에 대한 역학조사. 한국어병학회지, 20(1):61-70, 2007.
  26. 한국동물용 의약품판매협회: 동물용의약품등 편람. 2001.

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