DOI QR코드

DOI QR Code

장수버섯 배양으로 제조한 발효옻 추출물이 장류 미생물의 증식 및 효소활성에 미치는 영향

Effect of the Extracts from Fermented-Rhus verniciflua Stem Bark with Fomitella fraxinea on the Growth and Enzyme Activity of Soybean Product-fermenting Microorganisms

  • 최한석 (농촌진흥청 국립농업과학원 발효식품과) ;
  • 여수환 (농촌진흥청 국립농업과학원 발효식품과) ;
  • 정석태 (농촌진흥청 국립농업과학원 발효식품과) ;
  • 최지호 (농촌진흥청 국립농업과학원 발효식품과) ;
  • 강지은 (농촌진흥청 국립농업과학원 발효식품과) ;
  • 김명곤 (전북대학교 식품공학과)
  • Choi, Han-Seok (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Yeo, Soo-Hwan (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Jeong, Seok-Tae (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Choi, Ji-Ho (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Ji-Eun (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Myung-Kon (Department of Food Science and Technology, Chonbuk National University)
  • 투고 : 2012.10.30
  • 심사 : 2012.12.19
  • 발행 : 2012.12.31

초록

옻의 장류이용 가능성을 검토하기 위하여 urushiol이 제거된 발효옻 추출물이 장류발효미생물의 증식 및 효소활성에 미치는 영향에 대하여 확인하였다. 발효옻 알코올 추출물은 Bacillus 속과 Z. rouxii에 대하여 항균활성을 가지고 있었으나 열수 추출물은 Bacillus 속과 Z. rouxii를 포함한 젖산균, 효모, 대장균에 항균활성이 없었다. 발효 옻 열수 추출물은 L. plantarum, L. mesenteroides, S. cerevisiae의 균체성장에는 영향을 미치지 않았으나 Bacillus속에 특이적으로 작용하여 B. licheniformis, B. sutilis의 균체를 1.3-4.5배 증가시켰다. 그러나 Z. rouxii는 균체량을 감소시켰으며 유해 미생물인 B. cereus와 E. coli에 대한 증식억제 효과도 나타내지 않았다. 발효옻 열수 추출물은 B. licheniformis와 B. subtilis가 생산하는 amylase와 protease의 활성을 큰 폭으로 증가시켰으며, amylase는 B. licheniformis 11052P 균주에서 protease는 B. subtilis 10854에서 그 증가 폭이 가장 컸다. 지방 및 cellulose 분해활성은 B. licheniformis와 B. subtilis의 모든 균주에서 나타나지 않았다.

We studied the effect of fermented Rhus verniciflua stem bark (FRVSB) extract (used in herbal med-icine by Koreans) on the microbial growth and enzyme activity of 12 soybean-fermenting microorganisms, including Bacillus spp., lactic acid bacteria, yeast, and other harmful bacteria. The ethanol and methanol extracts of FRVSB inhibited the growth of Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, and Zygosaccharomyces rouxii, and in the disk diffusion assay, their inhibition zone diameters were 11.06-12.23, 12.32-18.38, 11.47-11.84, and 13.59-14.21 mm, respectively. The water extract did not show any inhibitory effect. In fact, the water extract addition enhanced the growth of B. subtilis and B. licheniformis by 1.3-4.5 fold and that of B. cereus by 1.2-1.4 fold. However, the water extract did not affect the growth of Lactobacillus plantarum, Lactobacillus mesenteroides, Saccharomyces cer-evisiae, and Escherichia coli. The addition of water extract increased the amylase and protease activity of B. subtilis and B. licheniformis.

키워드

참고문헌

  1. Choi, C. S. and Han, D. U. 2005a. The observation of the skin contact allergic sensitization test of Rhus-II with Guinea pig maximization test. J. Fd. Hyg. Safety. 20:13-17. (in Korean).
  2. Choi, C. S. and Han, D. U. 2005b. Genotoxicicological safety estimate for the Rhus-II. J. Fd. Hyg. Safety. 20:18-21. (in Korean).
  3. Choi, C. S. and Han, D. U. 2005c. Four-week repeated dose safety test of Rhus-II orally administrated to rats. J. Fd. Hyg. Safety 20:7-12. (in Korean).
  4. Choi, H. S., Kim, B. H., Yeo, S. H., Jeong, S. T., Choi, J. H., Park, H. S. and Kim, M. K. 2010a. Physicochemical properties and physiological activities of Rhus verniciflua Stem Bark Cultured with Fomitella fraxinea. Korean J. Mycol. 38:172-178. (in Korean). https://doi.org/10.4489/KJM.2010.38.2.172
  5. Choi, H. S., Kim, M. K., Park, H. S., Yun, S. E., Mun, S. P., Kim, J. S., Sapkota, K., Kim, S., Kim, T. Y. and Kim, S. J. 2007a. Biological detoxification of lacquer tree (Rhus verniciflua Stokes) stem bark by mushroom species. Food Sci. Biotechnol. 16:935-942.
  6. Choi, H. S., Yeo, S. H., Jeong, S. T., Choi, J. H., Park, H. S. and Kim, M. K. 2012. Preparation and characterization of urushiol free fermented Rhus verniciflua stem bark (FRVSB) extracts. Korean J. Food Sci. Technol. 44:173-178. (in Korean). https://doi.org/10.9721/KJFST.2012.44.2.173
  7. Choi, M. J., Lee, S. J., Jang, S. H., Reza, M. A., Hong, J. H., Jung, H. K. and Park, S. C. 2010b. Biological activities and single oral dose toxicity in rat of fermented Rhus verniciflua extract. Korean J. Vet. Res. 50:187-195. (in Korean).
  8. Choi, W. C., Park, S. J. and Kwon, S. P. 2007b. Process for the preparation of Rhus verniciflua extract having excellent anticancer activity and anti-cancer pharmaceutical composition containing same. Int. Patent WO/2007/049846.
  9. Dercova, K., Augustin, J. and Krajcova, D. 1992. Cell growth and a-amylase production characteristics of Bacillus subtilis. Folia Microbiol. 37:17-23. https://doi.org/10.1007/BF02814574
  10. Draper, W. M., Wijekoon, D., Makinney, M., Behniwal, P., Perera, S. K. and Flessel, C. P. 2002. Atmospheric pressure ionization LC-MS-MS determination of urushiol congeners. J. Agr. Food Chem. 50:1852-1858. https://doi.org/10.1021/jf011364t
  11. Eom, S. K. and Kim, K. S. 2008. On estimation of indication, property and processing of Rhus verniciflua Stokes. J. Oriental Med. Classics. 21:29-37. (in Korean).
  12. Epstein, W. L. 1974. Poison oak and poison ivy dermatitis as an occupational problem. Cuits 13:544-548.
  13. Fernald, M. L. 1950. Gray's Manual of Botany 8th ed., American Book Company, New York, USA. pp. 976-979.
  14. Gupta, R., Gigras, P., Mohapatra, H., Goswami, V. K. and Chauhan, B. 2003. Microbial a-amylases: a biotechnological perspective. Process Biochem. 38:1599-1616. https://doi.org/10.1016/S0032-9592(03)00053-0
  15. KFDA. 2010. Analytical Methods Of Korean Food Code. Korea Food & Drug Administration, Cheongwon, Korea. (in Korean).
  16. KFDA. 2012a. Food ingredients search. Available from: http:// fse.foodnara.go.kr/origin/search_data_list.jsp?srch_name =&edible=&animal=&codex=&query=%C0%E5%BC%F6%B 9%F6%BC%B8. Accessed Nov. 12, 2012.
  17. KFDA. 2012b. Notice No. 2012-204. Administrative notice of partial revision of the proposed criteria and standards for foods.
  18. KFS. 2008. 2007 the investigation of forestry management of Korea. Korea Forest Service, Daejeon, Korea. (in Korean).
  19. Kim, B. H. 2010. The effect of irradiation and peroxyacetic acid treatment on the reduction of urushiol of Rhus verniciflua Stokes and the physiological activities. MS thesis, Chonbuk National University, Gwangju, Korea. (in Korean).
  20. Kim, J. B. 2003. Identification of antioxidative component from stem bark of Rhus verniciflua. Korean J. Food Nutr. 16:60-65.
  21. Kim, J. S., Kwon, Y. S., Chun, W. J., Kim, T. Y., Sun, J., Yu, C. Y. and Kim, M. J. 2010. Rhus verniciflua Stokes flavonoid extracts have anti-oxidant, anti-microbial and $\alpha$- glucosidase inhibitory effect. Food Chem. 120:539-543. https://doi.org/10.1016/j.foodchem.2009.10.051
  22. Kim, M. J., Kim, C. J. and Kwak, S. S. 1997. Antifungal activity of urushiol components in the sap of Korean lacquer tree (Rhus verniciflua Stokes). Korean J. Plant Res. 10:231- 234. (in Korean).
  23. Kim, T. H., Lee, K. M., Kwon, K. R. and Choi, S. M. 2002. A literature study on lacquer poison. J. Korean pharmacopuncture institute 5:159-169. (in Korean). https://doi.org/10.3831/KPI.2002.5.1.159
  24. Lee, D. S., Jeong, G. S., Li, B., Park, H. and Kim, Y. C. 2010. Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. Int. Immunopharmacol. 10:850-858. https://doi.org/10.1016/j.intimp.2010.04.019
  25. Lee, J. and Parulekar, S. J. 1993. Enhanced production of $\alpha$- amylase in fedbatch cultures of Bacillus subtilis TN106[pAT5]. Biotechnol. Bioeng. 42:1142-1150. https://doi.org/10.1002/bit.260421003
  26. Lee, J. C., Kim, J., Lim, K. T. and Jang, Y. S. 2002. Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim. Biophys. Acta. 1570:181-191. https://doi.org/10.1016/S0304-4165(02)00196-4
  27. Lee, J. D., Huh, J. E., Jeon, G. S., Yang, H. R., Woo, H. S., Choi, D. Y. and Park, D. S. 2009. Flavonol-rich RVHxR from Rhus verniciflua Stokes and its major compound fisetin inhibits inflammation-related cytokines and angiogenic factor in rheumatoid arthritic fibroblast-like synovial cells and in vivo models. Int. Immunopharmacol. 9:268-276. https://doi.org/10.1016/j.intimp.2008.11.005
  28. Mabrouk, S. S., Hashem, A. M., El-Shayeb, N. M. A., Ismail, A. M. S. and Abdel-Fattah, A. F. 1999. Optimization of alkaline protease productivity by Bacillus licheniformis ATCC 21415. Biores. Technol. 69:155-159. https://doi.org/10.1016/S0960-8524(98)00165-5
  29. Nam, Y. D., Lee, S. Y. and Lim, S. I. 2012. Microbial community analysis of Korean pastes by next-generation sequencing. Int. J. Food Microbiol. 155:36-42. https://doi.org/10.1016/j.ijfoodmicro.2012.01.013
  30. Namba, T. 1980. Coloured illustrations of Wakan-Yaku. Hoikusha Publishing Co., Ltd., Osaka, Japan. p. 215.
  31. Park, K. Y., Jung, G. O., Lee, K. T., Choi, J. W., Choi, M. Y., Kim, G. T., Jung, J. J. and Park, H. J. 2004. Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. J. Ethnopharmacol. 90:73-79. https://doi.org/10.1016/j.jep.2003.09.043
  32. Patterson, S. E., Williams, J. V. and Marks, J. G. 1999. Prevention of sodium lauryl sulfate irritant contactdermatitis by Pro-Q aerosol foam skin protectant. J. Am. Acad. Dermatol. 40:783-785. https://doi.org/10.1016/S0190-9622(99)70168-4
  33. RDA. 2010. Process for production of urushiol free lacquer tree bark extract and its utilization. Korean Patent 10-2010- 0113258. (in Korean).
  34. RDA. 2011. Screening of functional substance and study of utilization technology from lacquer tree. Rural Development Administration, Suwon, Korea. (in Korean).
  35. Saary, J., Qureshi, R., Palda, V., DeKoven, J., Pratt, M., Skotnicki-Grant, S. and Holness, L. 2005. A systematic review of contact dermatitis treatment and prevention. J. Am. Acad. Dermatol. 53:845-855.
  36. Son, Y. O., Lee, K. Y., Lee, J. C., Jang, H. S., Kim, J. G., Jeon, Y. M. and Jang, Y. S. 2005. Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. Toxicol. Lett. 135:115-125.
  37. Sunchang-gun. 2012. Non-toxic new microbes having inhibitory activity growing for Bacillus cereus and sauce including them. Korean Patent 10-1132371-0000. (in Korean).
  38. Sunchang-gun, Chonbuk National Univ. R&BD foundation. 2012. Method for producing Chungkookjang Kochujang with reduced unpleasant odor. Korean Patent 10-1163386-0000. (in Korean).
  39. Vidmar, D. A. and Iwane, M. K. 1999. Assessment of the ability of the topical skin protectant (TSP) to protect against contact dermatitis to urushiol (Rhus) antigen. Am. J. Contact Dermat. 10:190-197. https://doi.org/10.1016/S1046-199X(99)90067-2
  40. Yoon, K. H. and Shin, H. Y. 2010. Medium optimization for the protease production by Bacillus licheniformis isolated from Cheongkookjang, Korean J. Microbiol. Biotechnol. 38:385-390. (in Korean).

피인용 문헌

  1. Changes Observed in Doenjang (Soybean Paste) Containing Fermented-Rhus verniciflua Extract During Aging vol.47, pp.5, 2015, https://doi.org/10.9721/KJFST.2015.47.5.599
  2. Quality characteristics of fermented vinegar prepared with the detoxified Rhus verniciflua extract vol.22, pp.5, 2015, https://doi.org/10.11002/kjfp.2015.22.5.674
  3. Quality characteristics of detoxified Rhus verniciflua vinegar fermented using different acetic acid bacteria vol.23, pp.3, 2016, https://doi.org/10.11002/kjfp.2016.23.3.347