Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Establishment of Suspension Culture System to Induce Somatic Embryo in Oplopanax elatus Nakai

현탁배양을 통한 땃두릅나무의 체세포배 유도

  • Kim, Hee Young (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Seong, Eun Soo (Bioherb Research Institute, Kangwon National University) ;
  • Lee, Jae Geun (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Yoo, Ji Hye (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Hwang, In Seong (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Kim, Myong Jo (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Na Young (Food Service Cuisine Songho College) ;
  • Yu, Chang Yeon (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
  • 김희영 (강원대학교 식물자원응용공학과) ;
  • 성은수 (강원대학교 한방바이오연구소) ;
  • 이재근 (강원대학교 식물자원응용공학과) ;
  • 유지혜 (강원대학교 식물자원응용공학과) ;
  • 황인성 (강원대학교 식물자원응용공학과) ;
  • 김명조 (강원대학교 식물자원응용공학과) ;
  • 임정대 (강원대학교 생약자원개발학과) ;
  • 김나영 (송호대학교 호텔외식조리과) ;
  • 유창연 (강원대학교 식물자원응용공학과)
  • Received : 2012.10.02
  • Accepted : 2012.11.09
  • Published : 2012.12.30
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Abstract

This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of $NH_4NO_3$ and $KNO_3$ at the ratio of 1650 (mg/${\ell}$) : 1900 (mg/${\ell}$) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with $170mg/{\ell}$ $KH_2PO_4$. The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.

Keywords

References

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