Asian Pacific Journal of Cancer Prevention

  • 제13권7호
  • /
  • Pages.3423-3426
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  • 2012
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Rapid Detection of Ovarian Cancer from Immunized Serum Using a Quartz Crystal Microbalance Immunosensor

  • Chen, Yan (School of Automation & Engineering, University of Electronic Science and Technology of China) ;
  • Huang, Xian-He (School of Automation & Engineering, University of Electronic Science and Technology of China) ;
  • Shi, Hua-Shan (State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Clinical Medicine School, Sichuan University) ;
  • Mu, Bo (North Sichuan Medical College) ;
  • Lv, Qun (Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital)
  • 발행 : 2012.07.31
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초록

Background: The objective of this study was to measure the antibody content of NuTu-19 ovarian cancer cells in serum samples using a quartz crystal microbalance (QCM) immunosensor. Materials and Methods: NuTu-19 cells were first cultured onto the electrode surfaces of crystals in Dulbecco's modified Eagle medium, and then specified amounts of immunized serum samples of immunized rabbit were also added. The change in mass caused by specific adsorbtion of antibodies of NuTu-19 to the surfaces of the crystals was detected. Results: The change in resonance frequency of crystals caused by immobilization of NuTu-19 cells was from 83 to 429Hz. The antibody content of NuTu-19 detected was 341ng/ul. The frequency shifts were linearly dependent on the amount of antibody mass in the range of 69 to 340ng. The positive detection rate and the negative detection rate were 80% and 100%, respectively. Conclusion: This immunoassay provides a viable alternative to other early ovarian cancer detection methods and is particularly suited for health screening of the general population.

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참고문헌

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피인용 문헌

  1. Early Lung Cancer Diagnosis by Biosensors vol.14, pp.8, 2013, https://doi.org/10.3390/ijms140815479
  2. Analysis and Improvement of the Mass Sensitivity for Piezoelectric Immunosensor vol.378, pp.1662-7482, 2013, https://doi.org/10.4028/www.scientific.net/AMM.378.435
  3. The Research on Loading Effect of Energy Transfer Model-Based QCM Immunosensor vol.401-403, pp.1662-7482, 2013, https://doi.org/10.4028/www.scientific.net/AMM.401-403.1149