Pediatric Infection and Vaccine

The Korean Society of Pediatric Infectious Diseases (대한소아감염학회)
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Phylogenetic Analysis of Human Bocavirus in Hospitalized Children with Acute Respiratory Tract Infection in Korea

급성 호흡기 감염으로 입원한 소아에서 분리된 보카바이러스의 계통분석

  • Ahn, Jong Gyun (Department of Pediatrics, School of Medicine, Ewha Womans University) ;
  • Choi, Seong Yeol (Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine) ;
  • Kim, Dong Soo (Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine) ;
  • Kim, Ki Hwan (Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine)
  • 안종균 (이화여자대학교 의과대학 소아과학교실) ;
  • 최성열 (연세대학교 의과대학 소아과학교실, 세브란스 어린이병원 소아청소년과) ;
  • 김동수 (연세대학교 의과대학 소아과학교실, 세브란스 어린이병원 소아청소년과) ;
  • 김기환 (연세대학교 의과대학 소아과학교실, 세브란스 어린이병원 소아청소년과)
  • Received : 2012.03.02
  • Accepted : 2012.04.27
  • Published : 2012.08.25
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Abstract

Purpose: Human bocavirus (hBoV), a recently discovered virus, has been detected in children with respiratory tract infections worldwide. The aim of this study was to analyze the frequency and molecular phylogeny of hBoV in the respiratory samples of children with acute respiratory tract infections in 2010. Methods: Nasopharyngeal samples were collected from 953 children with lower respiratory tract infections at Severance children's hospital in Korea from January 2010 to December 2010. We applied the multiplex PCR technique for the identification of 12 respiratory viruses from the samples. Among the total specimens, hBoV positive samples were subjected to phylogenetic analysis by sequencing a fragment of the VP1/VP2 gene junction. Results: hBoV was detected in 141 (14.8%) among 953 patients. The 61.7% of hBoV-positive samples were found to co-exist with other respiratory viruses. The results of phylogenetic analysis showed that all 141 hBoV-positive isolates were identified as hBoV 1, revealing a high similarity among the isolates (>98%). Conclusion: hBoV 1 with minimal sequence variations circulated in children with acute respiratory infections during 2010. More research is needed to determine the clinical severity and outcomes of the minimal sequence variations.

목적: 보카바이러스는 2005년 소아의 비인두 흡입물에서 처음으로 발견된 호흡기 바이러스로서 현재 전세계적으로 발견되고 있고 국내에서도 보카바이러스 호흡기 감염증이 보고되고 있다. 이번 연구는 중합효소 연쇄 반응(PCR)을 이용한 보카바이러스의 분리 및 유전자 분석을 통하여 2010년 국내에서 유행하였던 보카바이러스의 계통 및 유전적 변이를 알아보고자 하였다. 방법: 2010년 1월부터 2010년 12월까지 세브란스 어린이병원에 하기도 감염증으로 입원한 소아에서 비인두 흡입 검체를 채취하였다. 다중 중합효소 연쇄 반응을 이용하여 12종의 바이러스에 대한 분자진단이 이루어졌고 이 중 보카바이러스 양성인 검체에 대하여 보카바이러스 1-4형에 특이적인 VP1/2 유전자 염기서열을 기반으로 하는 시발체를 이용한 중합효소 연쇄 반응을 시행하여 얻은 증폭산물의 염기서열분석을 확인하여 계통 분석을 시행하였다. 결과: 전체 953명의 소아 중 141명(14.8%)에서 보카바이러스가 검출되었고, 61.7%에서 다른 바이러스와의 중복감염을 보였다. 염기서열분석 결과 모두 보카바이러스 1형으로 확인되었고 보카바이러스 2, 3, 4형은 검출되지 않았다. 검출된 모든 보카바이러스의 염기서열들은 98% 이상의 염기유사도를 보였다. 결론: 2010년 국내에서 유행한 보카바이러스 호흡기 감염은 유전학적으로 모두 보카바이러스 1형에 의한 것이었고, 동정된 균주들은 높은 염기유사도를 보였다. 아직까지 국내에서 보카바이러스의 변이는 미미한 상태이지만 향후 보카바이러스 호흡기 감염의 유행시 지속적인 유전자형의 추적관찰이 필요하다.

Keywords

References

  1. van den Hoogen BG, de Jong JC, Groen J, Kuiken T, de Groot R, Fouchier RA, et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 2001;7:719-24. https://doi.org/10.1038/89098
  2. Drosten C, Günther S, Preiser W, van der Werf S, Brodt HR, Becker S, et al. Identification of a novel coronavirus inpatients with severe acute respiratory syndrome. N Engl J Med 2003;348:1967-76. https://doi.org/10.1056/NEJMoa030747
  3. van der Hoek L, Pyrc K, Jebbink MF, Vermeulen-Oost W, Berkhout RJ, Wolthers KC, et al. Identification of a new human coronavirus. Nat Med 2004;10:368-73. https://doi.org/10.1038/nm1024
  4. Woo PC, Lau SK, Chu CM, Chan KH, Tsoi HW, Huang Y, et al. Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol 2005;79:884-95. https://doi.org/10.1128/JVI.79.2.884-895.2005
  5. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung- Lindell A, Andersson B. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A 2005;102:12891-6. https://doi.org/10.1073/pnas.0504666102
  6. Allander T, Jartti T, Gupta S, Niesters HG, Lehtinen P, Osterback R, et al. Human bocavirus and acute wheezing in children. Clin Infect Dis 2007;44:904-10. https://doi.org/10.1086/512196
  7. Fry AM, Lu X, Chittaganpitch M, Peret T, Fischer J, Dowell SF, et al. Humanbocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis 2007;195:1038-45. https://doi.org/10.1086/512163
  8. Bastien N, Brandt K, Dust K, Ward D, and Li Y. Human Bocavirus infection, Canada. Emerg Infect Dis 2006;12: 848-50. https://doi.org/10.3201/eid1205.051424
  9. Kapoor A, Slikas E, Simmonds P, Chieochansin T, Naeem A, Shaukat S, et al. A newly identified bocavirus species in human stool. J Infect Dis 2009;199:196-200. https://doi.org/10.1086/595831
  10. Arthur JL, Higgins GD, Davidson GP, Givney RC, Ratcliff RM. A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog 2009;5: e1000391. https://doi.org/10.1371/journal.ppat.1000391
  11. Kapoor A, Simmonds P, Slikas E, Li L, Bodhidatta L, Sethabutr O, et al. Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis 2010;201:1633-43. https://doi.org/10.1086/652416
  12. Chieochansin T, Kapoor A, Delwart E, Poovorawan Y, Simmonds P. Absence of detectable replication of human bocavirus species 2 in respiratory tract. Emerg Infect Dis 2009;15:1503-5. https://doi.org/10.3201/eid1509.090394
  13. Chow BD, Ou Z, Esper FP. Newly recognized bocaviruses (HBoV, HBoV2) in children and adults with gastrointestinal illness in the United States. J Clin Virol 2010; 47:143-7. https://doi.org/10.1016/j.jcv.2009.11.030
  14. Han TH, Chung JY, Hwang ES. Human bocavirus 2 in children, South Korea. Emerg Infect Dis 2009;15: 1698-700. https://doi.org/10.3201/eid1510.090337
  15. Santos N, Peret T, Humphrey C, Albuquerque M, Silva R, Benati F, et al. Human bocavirus species 2 and 3 in Brazil. J Clin Virol 2010;48:127-30. https://doi.org/10.1016/j.jcv.2010.03.014
  16. Chun JK, Lee JH, Kim HS, Cheong HM, Kim KS, Kang C, et al. Establishing a surveillance network for severe lower respiratory tract infections in Korean infants and young children. Eur J Clin Microbiol Infect Dis 2009;28: 841-4. https://doi.org/10.1007/s10096-009-0701-0
  17. Cheng W, Chen J, Xu Z, Yu J, Huang C, Jin M, et al. Phylogenetic and recombination analysis of human bocavirus 2. BMC Infect Dis 2011;11:50. https://doi.org/10.1186/1471-2334-11-50
  18. Choi EH, Lee HJ, Kim SJ, Eun BW, Kim NH, Lee JA, et al. The association of newly identified respiratory viruses with lower respiratory tract infections in Korean children, 2000-2005. Clin Infect Dis 2006;43:585-92. https://doi.org/10.1086/506350
  19. Chung JY, Han TH, Kim CK, Kim SW. Bocavirus infection in hospitalized children, South Korea. Emerg Infect Dis 2006;12:1254-6. https://doi.org/10.3201/eid1208.060261
  20. Choi CS, Park CH, Jung K, Lee G, Sun KK, Kim EY, et al. Clinical manifestation of human bocavirus infection in children. Korean J Pediatr Infect Dis 2007;14:136-44.
  21. Kim JS, Lim CS, Kim YK, Lee KN, Lee CK. Human Bocavirus in Patients with Respiratory Tract Infection. Korean J Lab Med 2011;31:179-84. https://doi.org/10.3343/kjlm.2011.31.3.179
  22. Choi JH, Paik JY, Choi EH, Lee HJ. Epidemiologic Characteristics of Human Bocavirus-Associated Respiratory Infection in Children. Korean J Pediatr Infect Dis 2011; 18:61-7.
  23. McIntosh K. Human bocavirus: developing evidence for pathogenicity. J Infect Dis 2006;194:1197-9. https://doi.org/10.1086/508228
  24. Song JR, Jin Y, Xie ZP, Gao HC, Xiao NG, Chen WX, et al. Novel human bocavirus in children with acute respiratory tract infection. Emerg Infect Dis 2010;16:324-7. https://doi.org/10.3201/eid1602.090553
  25. Foulongne V, Rodiere M, Segondy M. Human bocavirus in children. Emerg Infect Dis 2006;12:862-3. https://doi.org/10.3201/eid1205.051523
  26. Kesebir D, Vazquez M, Weibel C, Shapiro ED, Ferguson D, Landry ML, et al. Human bocavirus infection in young children in the United States: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus. J Infect Dis 2006;194:1276-82. https://doi.org/10.1086/508213
  27. Ma X, Endo R, Ishiguro N, Ebihara T, Ishiko H, Ariga T, et al. Detection of human bocavirus in Japanese children with lower respiratory tract infections. J Clin Microbiol 2006;44:1132-4. https://doi.org/10.1128/JCM.44.3.1132-1134.2006
  28. Chow BD, Esper FP. The human bocavirus: a review and discussion of their role in infection. Clin Lab Med 2009;29:695-713. https://doi.org/10.1016/j.cll.2009.07.010
  29. Naghipour M, Cuevas LE, Bakhshinejad T, Dove W, Hart CA. Human bocavirus in Iranian children with acute respiratory infections. J Med Virol 2007;79:539-43. https://doi.org/10.1002/jmv.20815