Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimization of Conditions for the Production of Alginate-degrading Crude Enzyme from Vibrio crassostreae PKA 1002

Vibrio crassostreae PKA 1002의 알긴산 분해 조효소 생산 최적 조건과 조효소의 특성

  • SunWoo, Chan (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Kim, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Jung, Seul-A (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Kim, Hyun-Jee (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Jeong, Da-Hyun (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Jung, Hee-Ye (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Lim, Sung-Mee (Department of Food Science & Technology Tongmyong University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
  • 선우찬 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과/식품연구소) ;
  • 김동현 (부경대학교 식품공학과/식품연구소) ;
  • 정슬아 (부경대학교 식품공학과/식품연구소) ;
  • 김현지 (부경대학교 식품공학과/식품연구소) ;
  • 정다현 (부경대학교 식품공학과/식품연구소) ;
  • 정희예 (부경대학교 식품공학과/식품연구소) ;
  • 임성미 (동명대학교 식품공학과) ;
  • 홍용기 (부경대학교 생물공학과) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2012.07.02
  • Accepted : 2012.08.28
  • Published : 2012.09.28
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Abstract

This study was conducted to screen an alginate-degrading microorganism and to investigate the characteristics of the alginate-degrading activity of its crude enzyme. A marine bacterium which produces extracellular alginate-degrading enzymes was isolated from the brown alga Sargassum thunbergii. 16S rRNA sequence analysis and physiological profiling resulted in the bacterium's identification as a Vibrio crassostreae strain, named Vibrio crassostreae PKA 1002. Its optimal culture conditions for growth were pH 9, 2% NaCl, $30^{\circ}C$ and a 24 hr incubation time. The optimal conditions for the alginate degrading ability of the crude enzyme produced by V. crassostreae PKA 1002 were pH 9, $30^{\circ}C$, a 48 hr incubation time and 8% alginic acid. The alginate degrading crude enzyme produced 3.035 g of reducing sugar per liter in 4% (w/v) alginate over 1 hr.

부산 송정 연안에서 해조류 및 해수로부터 알긴산 분해 미생물을 분리동정하고 미생물의 생육 조건 및 조효소액의 알긴산 분해 특성을 확인하였다. Sargassum thunbergii로부터 분리한 알긴산 분해균을 동정한 결과, Vibrio crassostreae strain로 확인 되었으며, V. crassostreae PKA 1002로 명명 하였다. V. crassostreae PKA 1002 최적 생육 조건을 확인한 결과, pH 9, 2% NaCl, $30^{\circ}C$ 및 배양 24 hr인 것으로 나타났으며, 최적 생육 조건에서 7% 알긴산과 1:1 배양시 환원당이 가장 많이 생성되는 것으로 나타났다. V. crassostreae PKA 1002를 최적 생육 조건으로 대량배양 후, 원심분리하여 얻은 상층액을 조효소액으로 하였으며, V. crassostreae PKA 1002 유래 알긴산 분해 조효소액은 pH 9, $30^{\circ}C$에서 분해 활성이 최대이며, 4% 알긴산 용액에서 1 hr 반응시 3.035 g/L의 환원당을 생성하는 것으로 나타나 추후 효소를 정제하고 알긴산 분해 특성을 구명하여 알긴산 올리고당 제조에 이용할 수 있을 것으로 사료되어 진다.

Keywords

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