The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-diabetic and Anti-Inflammatory Effects of Water Extract of Ligustrum japonicum Leaves in db/db Mouse

당뇨병 동물모델에서 여정엽(女貞葉) 추출물의 항당뇨 및 항염증 효과

  • Lee, Yun Jung (College of Oriental Medicine, Wonkwang University) ;
  • Lee, Yun Jae (Wonkwang University Oriental Jeonju Medicine Hospital) ;
  • Yoon, Jung Joo (College of Oriental Medicine, Wonkwang University) ;
  • Lee, So Min (College of Oriental Medicine, Wonkwang University) ;
  • Kim, Hye Yoom (College of Oriental Medicine, Wonkwang University) ;
  • Shin, Sun Ho (Wonkwang University Oriental Jeonju Medicine Hospital) ;
  • Kang, Dae Gill (College of Oriental Medicine, Wonkwang University) ;
  • Lee, Ho Sub (College of Oriental Medicine, Wonkwang University)
  • 이윤정 (원광대학교 한의과대학 생리학교실) ;
  • 이윤재 (원광대학교 한의과대학 전주한방병원) ;
  • 윤정주 (원광대학교 한의과대학 생리학교실) ;
  • 이소민 (원광대학교 한의과대학 생리학교실) ;
  • 김혜윰 (원광대학교 한의과대학 생리학교실) ;
  • 신선호 (원광대학교 한의과대학 전주한방병원) ;
  • 강대길 (원광대학교 한의과대학 생리학교실) ;
  • 이호섭 (원광대학교 한의과대학 생리학교실)
  • Received : 2012.09.03
  • Accepted : 2012.11.07
  • Published : 2012.11.30
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Abstract

Objectives : In this study, we investigated the anti-diabetic and anti-inflammatory effects of water extract from leaves of Ligustrum japonicum (WLJ) in db/db mouse. Methods : The db/db mice were treated orally with WLJ (300 mg/kg/day) for 10 weeks to examine the long-term effects on hyperglycemia and glomerular tissue as well as biochemical and functional abnormalities in the kidney. Results : WLJ treatment markedly reduced plasma levels of glucose, triglyceride, creatinine, and systolic blood pressure in diabetic db/db mouse. Treatment of WLJ significantly increased plasma level of high density lipoprotein (HDL)-cholesterol. We also found that overexpressions of vascular cellular adhesion molecule (VCAM)-1 and endothelin (ET)-1 were observed in aortic tissue of db/db mouse, whereas, WLJ suppressed both expression of VCAM-1 and ET-1 in aorta. In renal tissue, overexpressions of ICAM-1 and TGF-${\beta}1$ were found in untreated db/db mouse, however, significantly decreased those levels by WLJ treatment. The insulin immunoreactivity of the pancreatic islets remarkably increased in WLJ treated db/db mouse compared with untreated db/db mouse. Taken together, WLJ treatment ameliorated hyperglycemia and hyperlipidemia via improvement of insulin secretion and lipid metabolism, respectively. Furthermore, WLJ treatment also ameliorated hypertension via inhibition of inflammatory process in vascular and renal tissues. Conclusions : Ligustrum japonicum has an anti-diabetic and anti-inflammatory effects in db/db mouse. Thus, these results suggested a beneficial effect of Ligustrum japonicum in treatment with diabetes and diabetic vasculopathy.

Keywords

References

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