Identification of Self-incompatibility Genotypes of Apricot (Prunus armeniaca L.) by PCR and Test Crosses

  • Jun, Ji Hae (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwon, Jung Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chung, Kyeong Ho (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Ik-Koo (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yun, Seok-Kyu (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Yong-Uk (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwon, Soon Il (Apple Research Station, National Institute of Horticultural & Herbal Science, RDA)
  • 투고 : 2011.10.12
  • 심사 : 2011.11.30
  • 발행 : 2011.12.30

초록

Apricot (Prunus armeniaca L.) cultivars show a gametophytic self-incompatibility (GSI) system, like other fruit species of Rosaceae family. Thus, it is necessary to determine their S-genotypes in order for stable fruit set in commercial cultivation. S-genotypes of apricots were determined by PCR and test crosses. Three sets of consensus primers designed from Prunus S-RNases were used to amplify fragments containing the first and second S-RNase intron, respectively. Through the results obtained from the 3 PCRs, we could identify SI genotypes of 33apricot cultivars. Several cultivars such as 'Heiwa', 'Yamagata No.3' and 'Shinsuoomi' had the self-compatible (Sc) allele. Self-pollination tests revealed that cultivars with Sc allele were self-compatible. Cross-pollination tests confirmed that there was cross-incompatibility between the cultivars with the same S-genotypes. These results might be very useful for growers for effective pollination and for breeders using these in cross breeding programs.

키워드

참고문헌

  1. Alburquerque, N., Egea, J. Perez-Tornero, O. and Burgos, L. 2002. Genotyping apricot cultivars for self-incompatibility by means of RNases associated with S alleles. Plant Breeding 121:343-347. https://doi.org/10.1046/j.1439-0523.2002.725292.x
  2. Bailey, C.H. and Hough, L.F. 1975. Apricots, p. 367-383. In: J. Janick and J.N. Moore (eds.). Advances in fruit breeding. Purdue Univ. Press, West Lafayette, Ind.
  3. Beppu, K., Yamane, H., Yaegaki, H., Yamaguchi, M., Kataoka, I. and Tao, R. 2002. Diversity of S-RNase genes and S haplotypes in Japanese plum (Prunus salicina Lindl.). J. Hort. Sci. Biotech. 77:658-664.
  4. Beppu, K., Takemoto, Y., Yamane, H., Yaegaki, H., Yamaguchi, M., Kataoka, I. and Tao, R. 2003. Determination of S-haplotypes of Japanese plum (Prunus salicina Lindl.) cultivars by PCR and cross-pollination tests. J. Amer. Soc. Hort. Sci. 78:315-318.
  5. Burgos, L., Ledbetter, C.A., Pe'rez-Tornero, O., Orti'n-Pa rraga, F. and Egea, J. 1997. Inheritance of sexual incompatibility in apricot. Plant Breeding 116:383-386. https://doi.org/10.1111/j.1439-0523.1997.tb01016.x
  6. Chung, KH., Jun, JH. and Jeong, SB. 2004. Self- and cross-compatibility of some apricot cultivars (Prunus armeniaca L.). Kor. J. Hort. Sci. & Tech. 22: 261-265.
  7. De Nettancourt, D. 1977. Incompatibility in angiosperms. Monographs on theoretical and applied genetics. 3. Springer-Verlag, New York.
  8. Janssens, GA., Goderis, IJ., Broekaert, WF. and Broothaerts, W. 1995. A molecular method for S-allele identification in apple based on allele-specific PCR. Theor. Appl. Genet. 91:691-698.
  9. Jun, JH., Kwon, JH. and Chung, KH. 2007. Identification of self-incompatibility genotypes in Japanese plum (Prunus salicina Lindl.) by polymerase chain reaction and crosspollination tests. Hort. Environ. Biotechnol. 48:228- 234.
  10. Kao, TH. And Tsukamoto, T. 2004. The molecular and genetic bases of S-RNase-based self-incompatibility. Plant Cell 16:S72-S83. https://doi.org/10.1105/tpc.016154
  11. Lamb, R.C. and Stiles W.C. 1983. Apricots for New York state. New York Food & Life Sci. Bul. 100:1-4.
  12. McClure BA. and Franklin-Tong V. 2006. Gametophytic selfincompatibility : understanding the cellular mechanisms involved in 'self' pollen tube inhibition. Planta 224:233- 245. https://doi.org/10.1007/s00425-006-0284-2
  13. Ryutaro, T. and Amy, F.I. 2010. The S-RNase-based gametophytic self-incompatibility system in Prunus exhibits distinct genetic and molecular features. Scientia Horticulturae 124:423-433. https://doi.org/10.1016/j.scienta.2010.01.025
  14. Romero, C., Vilanova, S., Burgos, L., Martinez-Calvo, J., Vicente, M., Llacer, G. and Badenes, M.L. 2004. Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype S-RNase and F-box genes. Plant Mol. Biol. 56:145-157. https://doi.org/10.1007/s11103-004-2651-3
  15. Sambrook, J. and Russell, D.W. 2001. Molecular cloning : a laboratory manual. 3rd ed. Cold spring Harbor Lab. Cold spring Harbor, New York.
  16. Schultz, J.H. 1948. Self-incompatibility in apricots. Proc. Amer. Soc. Hort. Sci. 51:171-174.
  17. Sonneveld, T., Robbins, T.P., Boskovic, R. and Tobutt, K.R. 2001. Cloning of six cherry self-incompatibility alleles S1 to S16 using consensus and allele-specific primers. Theor. Appl. Genet. 107(6):1059-1070.
  18. Tamura, M., Ushijima, K., Sassa, H., Hirano, H., Tao, R., Gradziel, T.M., and Dandekar, A. M. 2000. Identification of self-incompatibility genotypes of almond by allelespecific PCR analysis. Theor. Appl. Genet. 101:344-349. https://doi.org/10.1007/s001220051489
  19. Tao, R., Yamane, H., Sugiura, A., Murayama, H., Sassa, H. and Mori. H. 1999. Molecular typing of S-alleles through identification, characterization and cDNA cloning for S-RNases in sweet cherry. J. Amer. Soc. Hort. Sci. 124:224-233.
  20. Vilanova, S., Romero, C., Llácer, G., Badenes, ML. and Burgos, L. 2005. Identification of self-(in)compatibility alleles in apricot by PCR and sequence analysis. J. Amer. Soc. Hort. Sci. 130:893-898.
  21. Zhang, LJ., Chen, XS., Chen, XL., Zhang, CY., Liu, XL., Ci, ZJ., Zhang, H., Wu, CJ. And Liu, CQ. 2008. Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars. Euphytica 160:241-248. https://doi.org/10.1007/s10681-007-9544-x