Korean Journal of Environment and Ecology (한국환경생태학회지)

Korean Society of Environment and Ecology (한국환경생태학회)
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Assessment of the Minimum Population Size for ex situ Conservation of Genetic Diversity in Aster altaicus var. uchiyamae Populations Inferred from AFLP Markers

AFLP 마커를 이용한 단양쑥부쟁이 개체군의 유전다양성 보전을 위한 최소개체군의 크기산정

  • 김창균 (아주대학교 자연과학부 생명과학과) ;
  • 김호준 (한국수자원공사) ;
  • 최홍근 (아주대학교 자연과학부 생명과학과)
  • Received : 2011.04.14
  • Accepted : 2011.08.31
  • Published : 2011.08.31
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Abstract

Aster altaicus var. uchiyamae is on the list of endangered species in Korea. Using amplified fragment length polymorphism (AFLP) markers, we investigated the genetic diversity within and among four populations (Guram, Dori Island, Samhap, and Danyang) of A. altaicus var. uchiyamae. We also present the collecting strategies that most efficiently capture the genetic diversity of A. altaicus var. uchiyamae. Four AFLP primer combinations produced a total of 936 bands, of which 934 (99.8%) were polymorphic. A high level of genetic diversity (PPB = 45.3%, h = 0.104, I = 0.168, hs = 0.108) was recognized within the populations of A. altaicus var. uchiyamae. A low degree of genetic differentiation ($G_{ST}$ = 0.075, ${\theta}^B$ = 0.079) was detected among the populations. In addition, analysis of molecular variance (AMOVA) showed that genetic variation was greater within populations (91%) than among populations (9%). These results indicate that the high rate of gene flow has played an important role in forming the present populations of A. altaicus var. uchiyamae. According to maximization strategy, 17, 16, and 11 individuals captured all of the genetic variation in Dori Island, Samhap, and Guram population, respectively. The determination the minimum population size of A. altaicus var. uchiyamae in terms of the genetic information is critical and thereby gain reliable decision support for ex situ conservation of the endangered species, A. altaicus var. uchiyamae.

본 연구는 멸종위기식물인 단양쑥부쟁이(Aster altaicus var. uchiyamae)의 개체군을 대상으로 유전다양성을 유지하는데 필요한 최소개체수를 산정하기 위하여 수행되었다. 단양쑥부쟁이가 분포하고 있는 네 지역에서 각각 유전다양성 및 유전적 분화도를 분석하였다. AFLP(amplified fragment length polymorphism) 마커를 이용한 유전적 변이의 분석결과, 총 4개의 프라이머 조합에 대해서 936개의 밴드가 확인되었으며, 그 중 934개의 밴드(99.8%)가 다형성을 보여주었다. 단양쑥부쟁이 개체군 내에서 유전다양성(PPB = 45.3%, h = 0.104, I = 0.168, hs = 0.108)은 높은 수준으로 나타났으며, 개체군 간 유전적 분화도($G_{ST}$ = 0.075, ${\theta}^B$ = 0.079)는 낮은 수준이었다. AMOVA(Analysis of molecular variance)분석 결과에서도 전체 유전적 변이 중 91%가 개체군 내에서 보이는 반면, 9%는 개체군 간 변이에 기인한 것으로 나타났다. 단양쑥부쟁이 개체군에서 보이는 유전적 특성은 개체군 간의 빈번한 유전자 이동에 기인한 것으로 사료된다. 최대화 전략법에 의하여 경기도 여주일대의 3개 개체군을 대상으로(굴암, 도리섬, 삼합) 개체군 내 최소개체수를 산정한 결과 도리섬개체군에서는 17개체, 삼합개체군에서는 16개체, 굴암개체군에서는 11개체로 파악되었다. 단양쑥부쟁이 개체군의 최소개체수에 대한 정보는 효율적인 현지 외 보전을 위한 가이드라인을 제시해 줄 수 있다.

Keywords

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