Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Paeonia lactiflora Pall Pprevents $H_2O_2$-induced Hepatotoxicity by Increasing HSP72 and HO-1

작약에 의해 유도되는 HSP72 및 HO-1 유전자의 간독성 보호 효능

  • Oh, Su-Young (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Lee, Ji-Seon (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Seo, Sang-Hee (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Tae-Soo (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine (KIOM))
  • Received : 2011.09.09
  • Accepted : 2011.10.04
  • Published : 2011.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

In Korea, China, and Japan, Paeonia lactiflora Pall (PL) has been used in the treatment of rheumatoid arthritis, hepatitis, and fever for more than 1200 years. It has been reported that PL has protective effects against $H_2O_2$-induced oxidative stress and LPS-induced liver inflammation. However cellular and molecular mechanism of PL protection against oxidative stress has not fully been elucidated. Here, we describe that the water-soluble extract of PL decreased $H_2O_2$-induced hepatotoxicity. This hepatoprotective effect of PL is reason to decrease the level of intracellular reactive oxygen species (ROS) and increase expression of heme oxygenase 1 (HO-1) and heat shock protein 72 (HSP72) which proteins are involved in protecting the cells from stress like as oxidative stress. We also elucidated that hepatoprotective effect of PL was abolished by knock down of HO-1 and HSP72 by siRNA. These results suggest that the increasing of HO-1 and HSP72 protein by PL treatment might be participated in hepatoprotective effect against oxidative stress such as $H_2O_2$.

Keywords

References

  1. 서상희, 이향림, 이순재, 최상원, 조성희. 작약 (Paeonia lactiflora Pall.) 씨 추출물과 Resveratrol이 흰쥐 체내 지질 상태에 미치는 영향. J. Korean Soc. Food Sci. Nutr. 32(7):1102-1107, 2003. https://doi.org/10.3746/jkfn.2003.32.7.1102
  2. Kim, I.D., Ha, B.J. The effects of paeoniflorin on LPS-induced liver inflammatory reactions. Arch. Pharm. Res. 33(6):959-966, 2010. https://doi.org/10.1007/s12272-010-0620-8
  3. Asai, M., Kawashima, D., Katagiri, K., Takeuchi, R., Tohnai, G., Ohtsuka, K. Protective effect of a molecular chaperone inducer, paeoniflorin, on the HCl- and ethanol-triggered gastric mucosal injury. Life Science. 88: 350-357, 2011. https://doi.org/10.1016/j.lfs.2010.12.014
  4. Zhong, S.Z., Ge, Q.H., Qu, R., Ma, S.P. Peoniflorin attentuates Abeta((1-42))-mediated neurotoxicity by regulating calcium homeostasis and ameliorating oxidative stress in hippocampus of rats. J. Neurol. Sci. 280(1-2):71-78, 2009. https://doi.org/10.1016/j.jns.2009.01.027
  5. Zhan, L.Y., Xia, Z.Y., Chen, C., Wang, X.Y. Effect of Radix Paeoniae Rubra on the expression of HO-1 and iNOS in rats with endotoxin-induced acute lung injury. Chin. J. Traumatol. 9(3):181-186, 2006.
  6. 이정민, 최상원, 조성희, 이순재. 작약 (Paeonia Lactiflora Pall)씨 추출물이 고콜레스테롤 식이 흰쥐 간조직의 항산화계와 지질과산화에 미치는 영향. Korean J. Nutr. 36(8):793-800, 2003.
  7. Lee, S.M., Yoon, M.Y., Park, H.R. Protective effects of Paeonia lactiflora Pall on hydrogen peroxide-induced apoptosis in PC12 cells. Biosci. Biotechnol. Biochem. 72(5):1272-1277, 2008. https://doi.org/10.1271/bbb.70756
  8. 문진영. 작약 약침액이 tert-butyl hydroperoxide로 유도된 흰 쥐 배양 간세포의 지질과산화반응 및 항산화효소 활성에 미 치는 영향. The Journal of Korean Acupuncture & Moxibustion Society. 17(3):176-187, 2009.
  9. Ndisang, J.F. Role of Heme Oxygenase in Inflammation, Insulin-Signalling, Diabetes and Obesity. Mediators of Inflammation. 2010: 1-18, 2010.
  10. Immenschuh, S., Baumgart-Vogt, E., Mueller, S. Heme oxygenase-1 and iron in liver inflammation: a complex alliance. Curr. Drug Targets. 11(12):1541-1550, 2010. https://doi.org/10.2174/1389450111009011541
  11. Moseley, P.L. Heat shock proteins and the inflammatory response. Annals of the New York Academy of Sciences. 856: 206-213, 1998. https://doi.org/10.1111/j.1749-6632.1998.tb08327.x
  12. Febbraio, M.A., Koukoulas, I. HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise. J. Appl. Physiol. 89(3):1055-1060, 2000. https://doi.org/10.1152/jappl.2000.89.3.1055
  13. 박영순. 한방의 약리해설. 서울, 아카데미서적, pp 155-156, 2002.
  14. 안덕균. 한국본초도감. 서울, 교학사, p 713, 2003.
  15. Chen, T., Guo, Z., Jiao, X., Zhang, Y., Li, J., Liu, H. Protective effects of peoniflorin against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells. Can. J. Physiol. Pharmacol. 89: 445-453, 2011. https://doi.org/10.1139/y11-034