The Journal of Advanced Prosthodontics

  • 제3권3호
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  • Pages.152-160
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  • 2011
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  • 2005-7806(pISSN)
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  • 2005-7814(eISSN)
대한치과보철학회 (The Korean Academy of Prosthodonitics)
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Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation

  • Huh, Jung-Bo (Department of Prosthodontics, School of Dentistry, Pusan National University) ;
  • Lee, Jeong-Yo (Department of Prosthodontics, Institute for Clinical Dental Research, Korea University Guro Hospital) ;
  • Lee, Kyung-Lae (Department of Prosthodontics, Institute for Clinical Dental Research, Korea University Guro Hospital) ;
  • Kim, Sung-Eun (Department of Orthopedic Surgery & Rare Diseases Institute, Korea University Medical Center, Guro Hospital) ;
  • Yun, Mi-Jung (Department of Prosthodontics, School of Dentistry, Pusan National University) ;
  • Shim, Ji-Suk (Department of Prosthodontics, Institute for Clinical Dental Research, Korea University Guro Hospital) ;
  • Shim, June-Sung (Department of Prosthodontics, School of Dentistry, Yonsei University) ;
  • Shin, Sang-Wan (Department of Prosthodontics, Institute for Clinical Dental Research, Korea University Guro Hospital)
  • 투고 : 2011.07.12
  • 심사 : 2011.08.09
  • 발행 : 2011.09.30
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초록

PURPOSE. This study was to investigate the effects of recombinant human platelet-derived growth factor (rhPDGF-BB) and heparin to titanium surfaces for enhancement of osteoblastic functions and inhibition of inflammation activity. MATERIALS AND METHODS. The anodized titanium discs, not coated with any material, were used as a control group. In heparinized-Ti group, dopamine was anchored to the surface of Ti substrates, and coated with heparin. In PDGF-Ti group, rhPDGF-BB was immobilized onto heparinized Ti surface. The surface morphologies were investigated by the scanning electron microscope in each group. The release kinetics of rhPDGF-BB were analyzed, and cytotoxicity tests for each group were conducted. The biocompatibilities were characterized by measuring cell proliferation, alkaline phosphatase activity, and calcium deposition using MG-63 cells. Statistical comparisons were carried out by one-way ANOVA tests. Differences were considered statistically significant at $^*$P<.05 and $^{**}$P<.001. RESULTS. The combination of rhPDGF-BB and heparin stimulated alkaline phosphatase activity and OCN mRNA expression in osteoblastic cells ($^*$P<.05 and $^{**}$P<.001). MG-63 cells grown on PDGF-Ti had significantly higher amounts of calcium deposition than those grown on anodized Ti ($^{**}$ P<.001). Heparinized Ti was more anti-inflammatory compared to anodized Ti, when exposed to lipopolysaccharide using the transcript levels of TNF-${\alpha}$ and IL-6 of proinflammatory cytokine ($^*$P<.05 and $^{**}$P<.001). CONCLUSION. The result of this study demonstrated that the incorporation of rhPDGF-BB and heparin onto Ti surface enhanced osteoblastic functions and inhibited inflammation.

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