Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Plant Regeneration from Mature Seed-Derived Callus in Bermudagrass (Cynodon dactylon)

난지형 목초 버뮤다그라스의 종자유래 캘러스로부터 식물체 재분화

  • 이기원 (농촌진흥청 국립축산과학원) ;
  • 박형수 (농촌진흥청 국립축산과학원) ;
  • 최기준 (농촌진흥청 국립축산과학원) ;
  • 김기용 (농촌진흥청 국립축산과학원) ;
  • 지희정 (농촌진흥청 국립축산과학원) ;
  • 김경희 (경상대학교 응용생명과학부) ;
  • 이병현 (경상대학교 응용생명과학부) ;
  • 이상훈 (농촌진흥청 국립축산과학원)
  • Received : 2011.06.30
  • Accepted : 2011.08.08
  • Published : 2011.09.30
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Abstract

The present study was conducted to determine the optimum in vitro culture condition for callus induction and plant regeneration from mature seeds of bermudagrass (Cynodon dactylon cv. Common). It was revealed that mature seeds cultured on MS medium supplemented with 2 mg/L 2,4-D, 0.5 g/L proline, 0.5 g/L casamino acid and 3 g/L Gelrite under light condition produced the highest percentage of callus formation (39.2%). The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 0.5 mg/L 2,4-D, 2 mg/L BA, 0.5 g/L proline, 0.5 g/L casamino acid 3 g/L Gelrite which induced the highest percentage of calli forming shoots (57.7%). The frequency of callus induction and plant regeneration were the highest on sucrose, followed by maltose. The shoots were rooted at the highest rate (100%) when transferred onto 1/2 MS medium. Regenerated plants were morphologically uniform with normal growth pattern.

버뮤다그라스는 현재 난지형 목초자원으로 많이 이용되고 있을 뿐만 아니라, 잔디용으로도 많이 이용되고 있는 품종 중의 하나이다. 그러나 난지형 목초인 버뮤다그라스는 동절기의 동해를 받아 일부 고사하거나 생육이 불량하며, 여름철 병해충 등의 발생으로 유지 관리가 어려운 단점이 있다. 따라서 버뮤다그라스의 이러한 문제점을 개선하기 위해서는 동해와 같은 각종 환경 스트레스에 잘 견디는 신품종개발이 시급한 실정이다. 본 연구에서는 버뮤다그라스의 성숙종자로부터 생장조절물질의 종류와 농도에 따른 캘러스 유도 및 식물체 재분화 효율을 조사하였다. 버뮤다그라스의 성숙종자배양에 있어서 캘러스 유도배지에 2 mg/L 2,4-D가 첨가된 처리구에서 39.2%로 가장 높게 나타났으며 식물체 재분화에는 0.5 mg/L 2,4-D와 2 mg/L BA가 첨가된 배지에서 배양함으로써 57.7% 이상의 높은 재분화율을 얻을 수 있었다. 캘러스유도와 식물체 재분화 배지에 필요한 배양 탄소원은 sucrose 첨가가 가장 효과적인 것으로 판명되었다. 본 연구를 통하여 확립된 성숙종자로부터 캘러스 유도 및 식물체재분화 시스템은 향후 각종 환경 스트레스에 대해 내성을 부여하는 유용 유전자의 도입을 통한 신품종 개발에 유용하게 이용될 것으로 판단된다.

Keywords

References

  1. Chaudhury, A. and Q. Rongda. 2000. Somatic embryogenesis and plant regeneration of turf type bermudagrass: effect of 6-benzyladenin in callus induction medium. Plant Cell Tiss. Org. Cult. 60:113-120. https://doi.org/10.1023/A:1006456005961
  2. Goldman, J.J., W.W. Hanna, G.H. Fleming and P. Ozias-Akins. 2004. Ploidy variation among herbicide-resistant bermudagrass plants of cv. TifEagle transformed with the bar gene. Plant Cell Rep. 22:553-560. https://doi.org/10.1007/s00299-003-0736-0
  3. Lee, K.-W., K.-Y. Kim, G.J. Choi, Y.C. Lim, W.H. Kim, M.W. Jung, S. Seo, B.-H. Lee and S.-H. Lee. 2008. Callus Induction and plant regeneration from mature seeds of timothy. J. Kor. Grassl. Forage Sci. 28:165-170. https://doi.org/10.5333/KGFS.2008.28.3.165
  4. Lee, K.-W., J.K. Lee, K.-Y. Kim, H.C. Ji, H.S. Park, K.-H. Kim, B.-H. Lee and S.-H. Lee. 2009a. Plant regeneration capacity of calluses derived from mature seed of perennial ryegrass cultivars. J. Kor. Grassl. Forage Sci. 29:285-290. https://doi.org/10.5333/KGFS.2009.29.4.285
  5. Lee, K.-W., G.J. Choi, K.-Y. Kim, H.C. Ji, H.S. Park, S.H. Yoon. and S.-H. Lee. 2009b. High frequency plant regeneration from mature seed derived callus of Italian ryegrass (Lolium multiflorum) cultivars. African Journal of Biotechnology. 8(24):6828-6833.
  6. Lee, S.-H., D.-G. Lee, J.-S. Kim and B.-H. Lee. 2003. High-frequency plant regeneration from mature seed-derived callus culture of orchardgrass. Kor J Plant Biotechnology. 30:341-346. https://doi.org/10.5010/JPB.2003.30.4.341
  7. Li, L. and R. Qu. 2002. In vitro somatic embyogenesis in turf-type bermudagrass roles of ABA and GA, and occurrence of repetitive somatic embryogenesis. Plant Breed. 121:155-158. https://doi.org/10.1046/j.1439-0523.2002.00684.x
  8. Li, L. and R. Qu. 2004. Development of highly regenerable callus lines and biolistic transformation of turf-type common bermudagrass [Cynodon dactylon (L.). Pers. ]. Plant Cell Rep. 22:403-407. https://doi.org/10.1007/s00299-003-0706-6
  9. Lu, S., Z. Guo and Y. Chen. 2003. Preliminary study on tissue culture of bermudagrass and its dwarf somaclonal variant. Acta Hort Sin. 30:482-484.
  10. Lu, S., Z. Wang, X. Peng, Z. Guo, G. Zhang and L. Han. 2006. An efficient callus suspension culture system for triploid bermudagrass (Cynodon transvaalensis . C. dactylon) and somaclonal variations. Plant Cell Tiss. Org. Cult. 87:77-84. https://doi.org/10.1007/s11240-006-9138-7
  11. Lu, S., X. Peng, Z. Guo, G. Zhang, Z. Wang, C. Wang, C. Pang, Z. Fan and J. Wang. 2007. In vitro selection of salinity tolerant variants from triploid bermudagrass (Cynodon transvaalensis . C. dactylon) and their physiological responses to salt and drought stress. Plant Cell Rep. 26:1413-1420. https://doi.org/10.1007/s00299-007-0339-2
  12. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Taliaferro, C.M. 1995. Diversity and vulnerability of bermuda turfgrass species. Crop Sci. 35:327-332. https://doi.org/10.2135/cropsci1995.0011183X003500020006x
  14. Zhang, S., W. Hanna and P. Ozias-Akins. 2007. Comparison of callus induction and plant regeneration from different explants in triploid and tetraploid turf-type bermudagrasses. Plant Cell Tiss. Org. Cult. 90:71-78. https://doi.org/10.1007/s11240-007-9255-y